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  4. KRT7 Monoclonal Antibody

KRT7 Monoclonal Antibody

  • 货号：
    CSB-MA634481
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-MA634481 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The KRT7 monoclonal antibody was produced using hybridoma technology, which involves fusing B cells from the spleen of a mouse immunized with a synthesized peptide derived from human KRT7 with myeloma cells. The resulting hybridoma cells were screened for the clones that produce KRT7 antibodies. These selected hybridoma cells were then cultured in the mouse abdominal cavity to produce KRT7 monoclonal antibodies. The KRT7 monoclonal antibody was purified from mouse ascites using affinity chromatography with a specific immunogen to ensure its purity. The purified antibody has been validated for use in ELISA and IHC applications.

    KRT7 is mainly expressed in epithelial cells, such as the cells lining the bladder, gastrointestinal tract, and bile ducts. Its main function is to provide structural support to these cells and to help maintain their mechanical integrity. KRT7 has been shown to play a role in cell signaling, cell differentiation, and cell migration, and it has also been implicated in the development of certain types of cancer.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) KRT7 Monoclonal antibody
  • Uniprot No.：
    P08729
  • 基因名：
    KRT7
  • 别名：
    CK 7 antibody; CK-7 antibody; CK7 antibody; Cytokeratin 7 antibody; Cytokeratin-7 antibody; D15Wsu77e antibody; K2C7 antibody; K2C7_HUMAN antibody; K7 antibody; Keratin 7 antibody; Keratin 7; type II antibody; Keratin type II cytoskeletal 7 antibody; Keratin; 55K type II cytoskeletal antibody; Keratin; simple epithelial antibody; Keratin; simple epithelial type I; K7 antibody; Keratin; type II cytoskeletal 7 antibody; Keratin-7 antibody; Krt2-7 antibody; KRT7 antibody; MGC11625 antibody; MGC129731 antibody; MGC3625 antibody; Sarcolectin antibody; SCL antibody; Type II mesothelial keratin K7 antibody; Type-II keratin Kb7 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Synthesized peptide derived from human Cytokeratin 7
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG1, Kappa
  • 纯化方式：
    The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
  • 基因功能参考文献：
    1. Data indicate that de novo expression of KRT7 and KRT19 resulting in altered plasticity and stem cell characteristics of epithelial cells might be a crucial factor in increasing the risk of tumor development in end-stage kidneys. PMID: 30194170
    2. High expression of CK-7 is associated with intrahepatic cholangiocarcinoma. PMID: 29513894
    3. Positive expression of CK7 associated with pathological features of lymph node metastasis and T stage may be independent clinical parameters for poor prognosis of patients with lung cancer. PMID: 28827446
    4. Research show that circulating CK7 mRNA positive cells were found in patients with bladder urothelial cancer of varying stages, and the detection rates increased with stage predicting worse clinical scenario. PMID: 28024950
    5. CK7/ HPV-L1 expression, and the presence of koilocytosis can be used for prognostication in patients with cervical low-grade squamous intraepithelial lesions. PMID: 28554573
    6. KRT7-AS formed an RNA-RNA hybrid with KRT7 and controlled KRT7 expression at both the mRNA and the post-transcriptional levels. forced overexpression of the KRT7-overlapping region (OL) of KRT7-AS (but not its non-KRT7-OL portions) increased keratin 7 protein levels in cells. Finally, forced overexpression of full-length KRT7-AS or OL KRT7-AS (but not its non-KRT7-OL regions) promoted GC cell proliferation PMID: 26876208
    7. These data support the theory that CK7 staining may inform risk stratification for low-grade squamous intraepithelial cervical lesions (CIN1). PMID: 27680604
    8. the status of K7 expression in metastatic lymph nodes from colorectal carcinoma is a poor prognostic factor PMID: 28155971
    9. Mismatch repair (MMR) defects influence the expression of clinically important biomarkers for endometrioid-type endometrial carcinoma as decreased cytokeratin 7 expression is more commonly associated with MMR deficiency. PMID: 25851713
    10. Ischemic parenchymal changes are characterized by hepatocyte K7 immunoexpression PMID: 26887669
    11. Survival analysis showed that the non-small cell lung carcinoma patients with enhanced expression of CK7, ELF3, EGFR, and EphB4 mRNA in peripheral blood leukocytes had poorer disease-free survival and overall survival than those without. PMID: 27827952
    12. Keratin 34betaE12/K7 expression is a prognostic parameter in resected early stage NSCLC that allows identification of high-risk NSCLC patients with poor cancer-specific and overall survival. PMID: 26057535
    13. K7 expression was also detected in 72 of 75 triple-negative carcinoma cases PMID: 26670478
    14. CK7, TTF-1 and napsin A are predominantly expressed in primary lung adenocarcinoma patients, with CDX-2 being inconsistently expressed. PMID: 26469326
    15. CK7 staining was notably heterogeneous, with 14.5% of all cases demonstrating
    16. Data show that cytokeratin-7 (KRT7) mRNA expression serves as a sensitive approach for the molecular detection of KRT7-positive circulating tumour cells (CTCs)-resembling A549 cells in peripheral whole blood. PMID: 26306784
    17. Cytokeratin 7 positivity in cervical low-grade squamous intraepithelial lesion is a marker for risk of progression to a high-grade lesion. PMID: 26551618
    18. Immunostaining for CK7 and epithelial membrane antigen can be used to differentiate interlobular bile ducts from ductular proliferation in patients with cholestasis. PMID: 26366614
    19. Epithelial-mesenchymal transition-related proteins CK-7 and alpha-SMA colocalized to the intrahepatic biliary epithelial cells in patients with biliary atresia. PMID: 25406900
    20. The immunofluorescent staining pattern of Wnt1 and CK7 as well as Wnt1 and CK13 was consistent with IHC results. Thus, in pleomorphic adenoma, Wnt is involved in tumor cell differentiation of peripheral columnar cells forming solid nests PMID: 25076852
    21. The present study confirmed that CK14, but not CK20 or CK7, is expressed in urothelial carcinoma with squamous differentiation and squamous cell carcinoma of the urinary bladder. PMID: 25643514
    22. Our results suggested that a combination of CK7 and TP53 immunohistochemistry may be helpful in diagnosing inflammatory bowel disease-associated dysplasia in difficult cases. PMID: 23887291
    23. BRAF-mutated microsatellite stable colorectal carcinoma often displays reduced CDX2 and increased CK7 expression PMID: 24908142
    24. Loss of cytokeratin 7 is associated with reduced response to concommitant radiochemotherapy for locally advanced cervical cancer. PMID: 24403459
    25. CK7 and Cam 5.2 expression may occur in SCC. A panel including Ber-Ep4 is advisable for immunohistochemical differentiation of EPD from SCC. PMID: 23590728
    26. Of the cases of clear cell renal carcinoma there was immunoreactivity for alpha-methylacyl-CoA racemase and strong diffuse immuno-positivity for cytokeratin. PMID: 23434146
    27. CK7 + centrilobular hepatocytes occur relatively frequently in non-neoplastic liver disease, associated with centrilobular scarring and CK7-positive periportal hepatocytes, and appear to be a non-specific phenomenon of underlying disease. PMID: 22716237
    28. Heterogeneity of cytokeratin 7 expression in pagetoid Bowen's disease. PMID: 22390404
    29. Pouch/peripouch and UC-associated adenocarcinoma had a comparable positive rate for CK7, CK20, and CDX2 by immunohistochemistry. PMID: 22895272
    30. High Cytokeratin-7 is associated with esophageal squamous cell carcinoma. PMID: 22203179
    31. Both the CK7-/CK20+ phenotype and expression of the antibody CDX2 are highly specific and sensitive markers of colorectal origin. PMID: 22268990
    32. This is the first reported study of the relationship between CK20/CK7 immunophenotype, BRAF mutations and microsatellite status in colorectal carcinomas PMID: 22361037
    33. None of the Wilms' tumors-associated lesions were positive for KRT7, but 69-80% of lesions associated with pRCpapillary renal cell tumors and mucinous tubular and spindle cell carcinomas were positive for KRT7. PMID: 22382985
    34. The expressions of CK7 and CK20 in nasal polyps were analyzed. PMID: 22119824
    35. Aberrant expression of K7 in budding cancer cells represents a modification of the epithelial phenotype ('epithelial-epithelial transition': EET) which may be linked to gains in motility and invasive potential. PMID: 21884201
    36. CK-7 expression grades correlated positively with histological stages of primary biliary cirrhosis (r=0.639, P<0.000) and negatively with granulomas (r=-0.432, P<0.0001; OR=0.173, P=0.0011). PMID: 21681009
    37. Our results reveal that menopause influences the adipose tissue expression of many genes, especially of neurexin 3, metallothionein 1E, and keratyn 7, which are associated with the alteration of several key biological processes. PMID: 21358552
    38. Our results along with the data from the literature indicate that CK7/CK20 expression may be of clinical significance. PMID: 21574103
    39. Case Report: Primary pulmonary adenocarcinoma with enteric differentiation resembling metastatic colorectal carcinoma, negative for cytokeratin 7. PMID: 20727680
    40. A considerable number of colorectal carcinomas showed immunoreactivity to CK7. PMID: 21282015
    41. Hepatocyte CK7 expression is frequently noted in chronic allograft rejection, and it would appear to reflect ductopenia. PMID: 21228364
    42. Immunohistochemistry for cytokeratins 7 and 19, which mark biliary epithelium, is helpful in the diagnosis of biliary diseases. PMID: 20538416
    43. Endometrial adenocarcinomas show micro-anatomical variations in Ki67 expression and this is often inversely correlated with CK7 immunoreactivity. PMID: 20557372
    44. Case Report: CK7+/CK20- Merkel cell carcinoma presenting as inguinal subcutaneous nodules with subsequent epidermotropic metastasis. PMID: 20574624
    45. the expression of Cytokeratins 7, 8, 18, and 19 may serve as differential diagnostic markers for pulmonary large cell neuroendocrine carcinoma and small cell lung carcinoma PMID: 20398190
    46. CK7 is a possible marker for colorectal carcinogenesis. PMID: 17715023
    47. FOXA1 induces not only KRT7 but also LOXL2 in a subset of poor prognostic esophageal squamous cell carcinomas with metastatic lymph nodes PMID: 20043065
    48. Toker cells and mammmary Paget cells share immunoreactivity to CK7. PMID: 20001343
    49. Changing pattern of cytokeratin 7 and 20 expression from normal epithelium to intestinal metaplasia of the gastric mucosa and gastroesophageal junction PMID: 11962749
    50. HPV16 E7 mRNA-cytokeratin 7 binding in squamous cervical cancer SiHa cells occurs through the 6-mer peptide SEQIKA present in human cytokeratin 7 protein PMID: 12072504

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
  • 数据库链接：

    HGNC: 6445

    OMIM: 148059

    KEGG: hsa:3855

    STRING: 9606.ENSP00000329243

    UniGene: Hs.411501