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CACYBP Antibody

  • 货号：
    CSB-PA872536LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate
      All lanes: CACYBP antibody at 2.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 27, 9, 22 kDa
      Observed band size: 27 kDa
    • Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA872536LA01HU at dilution of 1:100
    • Immunofluorescence staining of U251 cells with CSB-PA872536LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating CACYBP in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA872536LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA872536LA01HU (8μg) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (10μg)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) CACYBP Polyclonal antibody
  • Uniprot No.：
    Q9HB71
  • 基因名：
  • 别名：
    CacyBP antibody; Calcyclin binding protein antibody; Calcyclin-binding protein antibody; CYBP_HUMAN antibody; GIG 5 antibody; GIG5 antibody; Growth inhibiting gene 5 antibody; Growth inhibiting gene 5 protein antibody; hCacyBP antibody; MGC87971 antibody; PNAS 107 antibody; PNAS107 antibody; RP1 102G20.6 antibody; S100A6 binding protein antibody; S100A6-binding protein antibody; S100A6BP antibody; Siah-interacting protein antibody; SIP antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Calcyclin-binding protein (2-228AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，CACYBP Antibody (CSB-PA872536LA01HU)，的标记方式是Non-conjugated。对于CACYBP Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA872536LB01HU CACYBP Antibody, HRP conjugated ELISA
    FITC CSB-PA872536LC01HU CACYBP Antibody, FITC conjugated
    Biotin CSB-PA872536LD01HU CACYBP Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • 基因功能参考文献：
    1. CacyBP expression is regulated by E2F1, EGR1, and CREB transcription factors in colorectal cancer HCT116 cells. PMID: 29197151
    2. Results suggest that CacyBP/SIP plays an important role in inhibiting glioma cell migration and invasion through promoting the degradation of cytoplasmic p27. PMID: 29024247
    3. Data show that S100 calcium binding protein A6 (S100A6) is required for the Ca2+-dependent nuclear translocation of calcyclin binding protein (CacyBP/SIP) in colon cancer SW480 cells. PMID: 29534068
    4. CacyBP/SIP nuclear localization, dependent on S100 protein, suppresses gastric cancer tumorigenesis through beta-catenin degradation and the dephosphorylation of ERK1/2 during the G2 phase. PMID: 29099417
    5. Our data have shown for the first time the regulation of CacyBP/SIP gene expression by NFAT1. Since NFAT transcription factors are involved in processes related to immune response, these results indicate potential involvement of CacyBP/SIP in the immune system. PMID: 28526484
    6. These results suggest that CacyBP/SIP may be promoting growth of colon cancer cells by enhancing ubiquitin-mediated degradation of p27kip1. PMID: 28196083
    7. The biological characteristics and target proteins of CacyBP/SIP and its exact role in various cancers are discussed. Review. PMID: 26873490
    8. CacyBP/SIP nuclear translocation contributes to the proliferation of gastric cancer cells, and CacyBP/SIP exerts this effect, at least in part, by stimulating ubiquitin-mediated degradation of p27Kip1. PMID: 27099442
    9. CacyBP/SIP plays an important role in inhibiting apoptosis of glioma cells which might be mediated by ERK1/2 signaling pathway. PMID: 26825673
    10. CacyBP/SIP is a useful indicator of dis processes in Chronic Lymphocytic Leukemia (CLL) and plays an important role in sustaining the balance of cell proliferation and apoptosis. PMID: 26603518
    11. CacyBP/SIP nuclear translocation promotes the proliferation and cell cycle progression of gastric cancer cells. PMID: 25110433
    12. Overexpression of CacyBP is associated with glioma. PMID: 24740456
    13. This study presents CacyBP as a promising candidate biomarker for colorectal cancer (CRC) metastasis and also sheds light on the underlying molecular mechanism by which CacyBP promotes CRC metastasis. PMID: 23543800
    14. CacyBP enhances multidrug resistance of pancreatic cancer cells by regulation of P-gp and Bcl-2. PMID: 23463283
    15. These findings reveal a novel function for SNRK in the regulation of colon cancer cell proliferation and beta-catenin signaling. PMID: 22874833
    16. different activity of CacyBP/SIP in neuroblastoma NB2a and colon cancer HCT116 cells might affect the ERK1/2 pathway in the differentiation or proliferation processes PMID: 22480271
    17. new insight into the interaction between S100 proteins and CacyBP/SIP PMID: 22295074
    18. Data show that CacyBP/SIP might develop into another possible therapeutic target. PMID: 21268134
    19. Poor cellular differentiation, lymph node invasion, and clinicopathological staging in breast cancer were associated with CacyBP/SIP expression. PMID: 20585948
    20. CacyBP/SIP exhibits a phosphatase activity toward ERK1/2 kinases while its E217K mutant does not. PMID: 21110948
    21. CACYBP is associated with acute lung injury in mice PMID: 21297076
    22. CacyBP regulation of COX-2 expression may play an important role in human breast carcinogenesis PMID: 20878073
    23. investigation of interaction with Siah1 PMID: 12421809
    24. the translocation of CacyBP during the retinoic acid-induced differentiation of neuroblastoma SH-SY5Y cells suggested that this protein might play a role in neuronal differentiation PMID: 12895292
    25. SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in cells PMID: 16085652
    26. Taken together, our work showed that CacyBP/SIP, as a novel down-regulated gene in renal cell carcinoma, suppressed proliferation and tumorigenesis of renal cancer cells. PMID: 17400182
    27. CacyBP/SIP may be a potential inhibitor of cell growth and invasion in the gastric cancer cell, at least in part through the effect on beta-catenin protein expression and transcriptional activation of Tcf/LEF PMID: 18171983
    28. The first study on the CacyBP/SIP expression pattern in a broad range of human normal and tumor tissues. PMID: 18443365
    29. CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer. PMID: 18765951

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
  • 数据库链接：

    HGNC: 30423

    OMIM: 606186

    KEGG: hsa:27101

    STRING: 9606.ENSP00000356652

    UniGene: Hs.508524