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HRH4 Antibody

  • 货号：
    CSB-PA887972LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse liver tissue
      All lanes: HRH4 antibody at 4μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 45, 35 kDa
      Observed band size: 45 kDa
    • IHC image of CSB-PA887972LA01HU diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA887972LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) HRH4 Polyclonal antibody
  • Uniprot No.：
    Q9H3N8
  • 基因名：
  • 别名：
    HRH4; GPCR105; Histamine H4 receptor; H4R; HH4R; AXOR35; G-protein coupled receptor 105; GPRv53; Pfi-013; SP9144
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Histamine H4 receptor protein (204-292AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，HRH4 Antibody (CSB-PA887972LA01HU)，的标记方式是Non-conjugated。对于HRH4 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA887972LB01HU HRH4 Antibody, HRP conjugated ELISA
    FITC CSB-PA887972LC01HU HRH4 Antibody, FITC conjugated
    Biotin CSB-PA887972LD01HU HRH4 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
  • 基因功能参考文献：
    1. these findings reveal that rs11662595 is a loss-of-function polymorphism that results in dysfunction of HRH4 and attenuates the anti-epithelial-to-mesenchymal transition function of HRH4 in non-small cell lung cancer (NSCLC), which provides a promising biomarker for prognosis and therapy of NSCLC. PMID: 28847511
    2. Taken together, the silencing of H4R inhibited the H4R mediated Mast cell functions and SAPK/JNK phosphorylation. Furthermore, the H4R activation utilized SAPK/JNK signaling pathway for IL-1beta release in HMC-1 cells PMID: 29863427
    3. Our novel scientific data provide further indication that histamine, in particular via the H4R, plays a potent role in the pathophysiology of allergic diseases through tight control of Th1-Th2 mediator production favoring Th2 lymphocyte accumulation in inflamed tissues. Our results add to a growing evidence of data suggesting that the H4R may serve as a therapeutic target in allergic diseases. PMID: 29600327
    4. The expression of H4R was increased in peripheral blood monocytes in rheumatoid arthritis patients. Histamine and Th17 cytokines induced the osteoclast differentiation from monocytes and JNJ7777120 decreased the osteoclastogenesis. PMID: 28446753
    5. H1R and H4R are useful biomarkers of allergic inflammation on the ocular surface. Most notably, H4R expressed on eosinophils is useful as a biomarker of eosinophilic inflammation of the ocular surface. PMID: 28391980
    6. Histamine stimulation influences the IL-17 pathway in psoriasis via the fourth histamine receptor subtype, H4R, on CD4+ T cells. PMID: 28592369
    7. This study suggests that genetic variations within the HRH4 gene might be associated with special clinical features of psoriasis. PMID: 27725312
    8. Our findings provide evidence that HRH4 rs77485247 and rs77041280 polymorphisms may be associated with the risk of allergic rhinitis (AR) and the efficacy of H1 antihistamines for the treatment of AR patients. PMID: 27888642
    9. These data imply that the H4R regulates IgE-dependent processes in human basophils and provides a novel function of the H4R preventing an overwhelming immune reaction by engagement of a negative feedback loop. PMID: 26948974
    10. Pharmacological or genetic modulations of H2 and H4 HRs (H2R and H4R) not only suppressed gefitinib-induced cytostasis and differentiation of AML cells but also blocked EGFR and ERK1/2 inhibition in MDA-MB-231 cells PMID: 27180173
    11. Histamine H4 receptors could not be identified in five experimental models of the guinea-pig that are suited for the detection of presynaptic inhibitory receptors (hippocampus aorta, atrium, renal cortex and vas deferens) whereas H3 receptors could be shown in the peripheral tissues but not in the hippocampus PMID: 26211976
    12. Taken together these data demonstrate that compound A and compound L may block H4R-mediated downstream signaling events. PMID: 27807993
    13. We also found that the activation of H4R caused the release of IL-13 and RANTES on human mast cells.these data demonstrate that the H4R activates divergent signaling pathways to induce cytokine and chemokine production in human mast cells PMID: 27400655
    14. H4R may be involved in the maintenance of healthy oral mucosa. In oral lichen planus, this maintenance might be impaired by mast cell degranulation and inflammatory cytokines. PMID: 25207698
    15. Activation of the H4R could induce phosphorylation of ERK. PMID: 26828993
    16. Suggest roles for HRH4 polymorphisms and ankylosing spondylitis susceptibility. PMID: 26823878
    17. H4 receptor expression plays a role in pathological vessel leakage associated with choroidal neovascularization PMID: 24787705
    18. Molecular modelling studies, including molecular dynamic simulations and calculation of Gibbs energy of solvation of hH3R and hH4R, were studied. PMID: 25098339
    19. Functional H4 receptors increasing (35)S-GTPgammaS binding and/or decreasing noradrenaline release are not found in human, guinea pig or mouse cortex PMID: 25300787
    20. HRH4 was increased in clinically-isolated syndrome and different stages of multiple sclerosis compared to health control. PMID: 25293806
    21. No evidence was found for the presence of histamine H4 receptor in monocytes. PMID: 25273276
    22. In neutrophils, the H4 receptor may block signals emanating from Mac-1-controlling degranulation. Engagement of the H4 receptor by selective agonists blocked Mac-1-dependent activation of p38 MAPK. PMID: 24799603
    23. The activation of H4R in human mast cells produced not only inflammatory mediators that are associated with allergic reactions but also other inflammatory conditions. PMID: 24934979
    24. Increased expression of the H4 receptor was observed in HaCaT cells following differentiation. PMID: 24372819
    25. Histamine through the histamine H4 receptor exhibits a crucial role in breast tumour progression. PMID: 23425150
    26. Obtained results allow for statement that developed cellular model may be successfully employed in search for new compounds active at histamine H4 receptor PMID: 24432340
    27. Methyl substitution in histamine homologs offers a way to shift the selectivity in favor of the H4R. PMID: 24493592
    28. But carriers of one or three copies of HRH1 (5% of individuals), HRH2 (1.1%) and HRH4 genes (0.9%) were also identified. PMID: 24236486
    29. Data show down-regulation of cyclic adenosine monophosphate (cAMP) was the secondary signalling after H4 receptor activation, which in turn resulting in inactivation of transforming growth factor-beta1(TGF-beta1) pathway. PMID: 24447834
    30. The inhibitory effects of histamine on reactive oxygen species production in whole blood phagocytes are caused by H2R rather than H4R histamine receptors. PMID: 24530738
    31. H4R is highly expressed on keratinocytes from patients with atopic dermatitis, and its stimulation induces keratinocyte proliferation. PMID: 23932072
    32. In this review, we focus on the role of histamine and its receptors in the treatment of Alzheimer's disease. PMID: 23677734
    33. Copy number variationss of the HRH4 gene are associated with atopic dermatitis in a Chinese population. PMID: 23517361
    34. Histamine receptor-4-mRNA expression showed a significant increase in caudate nucleus and putamen in Parkinson's disease patients. PMID: 22118942
    35. Genotypes of HRH4 gene are signi fi cantly associated with the risk and malignant degree of breast cancer in Chinese Han populations. PMID: 23481304
    36. The efficacy of 31 known hHR ligands to induce Galpha(i) protein signaling and beta-arrestin2 recruitment by the hHR. PMID: 22973061
    37. Report down-regulation of HRH4 mRNA in synovial tissue from rheumatoid arthritis patients compared to those with osteoarthritis. PMID: 21881994
    38. Twenty-one SNPs of the HRH4 gene were genotyped in 313 asthmatic patients and 360 controls. Genotype distribution of three SNPs rs17187619, rs527790 and rs487202 differed significantly between patients with or without infection-induced asthma. PMID: 22653292
    39. study identified novel functional properties in terms of voltage sensitivities and deactivation rates, which differed between the histamine hH3445, hH3365, and H4 receptors PMID: 22885137
    40. analysis of fragment optimization and analysis of binding kinetics for ligand based design of novel histamine H receptor antagonists PMID: 22153663
    41. Deletion of HRH4 gene is present in gastric cancer cases and is closely correlated with attenuated gene expression. PMID: 22363581
    42. fundamental concepts of HR structure modeling and its implementation in drug discovery (review) PMID: 22201741
    43. histamine H(4) receptor as an attractive target in the treatment of inflammatory and autoimmune disorders PMID: 21741967
    44. Histamine H4 receptors were found in normal nasal mucosa, and increased significantly in nasal mucosa of allergic rhinitis patients. PMID: 21171298
    45. our results indicate that the H(4)R is highly expressed on plasmacytoid dendritic cells in psoriasis and influences cytokine production and migration of these cells PMID: 21614010
    46. These findings suggested a potential role of abnormal HRH4 expression in the progression of CRCs PMID: 21609450
    47. we describe a possible genetic impact on the expression level of the histamine H4 receptor and summarize the current data regarding the activity of the histamine H4 receptor on the key effector cells in atopic dermatitis PMID: 21104170
    48. slan-dendritic cells (slanDC) express the H(4) R and its stimulation leads to reduced pro-inflammatory capacity of slanDC PMID: 20722760
    49. Copy number variations of the human histamine H4 receptor gene are associated with systemic lupus erythematosus PMID: 20618322
    50. Langerhans cells express a functional H(4)R and point towards a possible pathogenic relevance of the H(4)R in inflammatory and allergic diseases. PMID: 19958313

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, inclu
  • 数据库链接：

    HGNC: 17383

    OMIM: 606792

    KEGG: hsa:59340

    STRING: 9606.ENSP00000256906

    UniGene: Hs.287388