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PSENEN Antibody

  • 货号：
    CSB-PA878932OA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA878932OA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA878932OA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) PSENEN Polyclonal antibody
  • Uniprot No.：
    Q9NZ42
  • 基因名：
  • 别名：
    PSENEN; PEN2; MDS033; Gamma-secretase subunit PEN-2; Presenilin enhancer protein 2
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Peptide sequence from Human Gamma-secretase subunit PEN-2 protein (39-57AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，PSENEN Antibody (CSB-PA878932OA01HU)，的标记方式是Non-conjugated。对于PSENEN Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA878932OB01HU PSENEN Antibody, HRP conjugated ELISA
    FITC CSB-PA878932OC01HU PSENEN Antibody, FITC conjugated
    Biotin CSB-PA878932OD01HU PSENEN Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity.
  • 基因功能参考文献：
    1. High gamma-secretase expression is associated with head and neck squamous cell carcinoma. PMID: 29047105
    2. APP substrate occupancy of these three pockets of gamma-secretase occurs after initial substrate binding but precedes catalysis, suggesting a conformational change in substrate may be required for cleavage. PMID: 27580372
    3. PSENEN mutations can indeed cause a comanifestation of Dowling-Degos disease and acne inversa (AI) that is likely triggered by predisposing factors for AI. PMID: 28287404
    4. zinc, copper inhibited Abeta production by directly targeting the subunits presenilin and nicastrin in the gamma-secretase complex PMID: 28096459
    5. PSENEN may play a crucial role in the progression of atopic dermatitis by participating in the Notch signaling pathway. PMID: 26967585
    6. Remarkably, PEN-2 was identified besides nicastrin as additional substrate-binding subunit. PMID: 27220847
    7. Results suggest deltaOR-Phe27Cys variation modulates beta- and gamma-secretase activity in late-stage Alzheimer's disease likely via post-translational mechanisms PMID: 26402014
    8. TRPC6 specifically interacts with APP leading to inhibition of its cleavage by gamma-secretase and reduction in Abeta production. PMID: 26581893
    9. secondary mutations in presenilin 1 alone activated the gamma-secretase activity. PMID: 26559975
    10. Both human PS2V and zebrafish PS1IV can stimulate gamma-secretase activity despite extreme structural divergence PMID: 25814654
    11. Data indicate that familial Alzheimer's disease (FAD) and control brain samples had similar overall gamma-secretase activity levels, and therefore, loss of overall (endopeptidase) gamma-secretase function cannot be an essential part of the pathogenic mechanism. PMID: 26481686
    12. We for the first time identify PEN-2 as the causative gene of familial comedones. PMID: 26044244
    13. The first hydrophobic domain of Pen-2 forms a structure similar to a reentrant loop while the second hydrophobic domain spans the lipid bilayer. PMID: 26296997
    14. shedding of BCMA by gamma-secretase controls plasma cells in the bone marrow and yields a potential biomarker for B-cell involvement in human autoimmune diseases PMID: 26065893
    15. Tumor necrosis factor-alpha and interleukin-10 levels were elevated in acne inversa patients with nicastrin or presenilin enhancer mutation. PMID: 26067312
    16. SLC2A13 is a novel gamma-secretase associated protein that regulates amyloid beta production without affecting Notch cleavage. PMID: 26094765
    17. Brain proteins showing neuron-specific interactions with gamma-secretase PMID: 25893612
    18. A complete inhibition of PS1-induced apoptosis was achieved by knockdown of PS1-associated protein (PSAP), a mitochondrial proapoptotic protein that forms a complex with Bax upon induction of apoptosis, in the presence of gamma-secretase inhibitor. PMID: 26025363
    19. analysis of how the conformation of presenilin, Pen-2, Aph-1, and nicastrin affect the function and mechanism of gamma-secretase PMID: 25918421
    20. Mutation of on PS1 and PS2 AXXXAXXXG motifs strongly impacts gamma-secretase activity. PMID: 25614624
    21. The catalytic subunit of gamma-secretase is presenilin 1 (PS1), which contains an initial substrate-binding site that is distinct from the catalytic site PMID: 25673856
    22. Recombinant human Pen-2 fusion protein was purified from bacteria to >95% purity. PMID: 24865334
    23. Together, our results suggest that iron can increase gamma-secretase activity through promoting the level of FTL that interacts with and stabilizes PEN-2/ PMID: 23685131
    24. A review of mutations in the gamma-secretase genes NCSTN, PSENEN, and PSEN1 and the role of gamma-secretase in cutaneous biology and, more specifically in hidradenitis suppurativa. PMID: 23096707
    25. Allele A of Pen 2 gene may increase the risk of late onset Alzheimer's disease. PMID: 23134962
    26. a 269 bp region located between the PSENEN and U2AF1L4 human genes is a genuine bidirectional promoter regulating a concerted divergent transcription of these genes. PMID: 23246698
    27. Mutations in the gamma-secretase genes NCSTN, PSENEN, and PSEN1 underlie rare forms of hidradenitis suppurativa (acne inversa). PMID: 22622421
    28. The molecular state of gamma-secretase and its enzymological characteristics are described. PMID: 22787762
    29. secretase subunits restrict the arrangement of the transmembrane domains of presenilin during the formation of the functional structure of the catalytic pore. PMID: 22689582
    30. Studies indicate that gradual saturation of gamma-secretase with its substrate can be the pathogenic process in different alleged causes of Alzheimer's disease (AD). PMID: 22479317
    31. expression of calsenilin leads to a disruption of presenilin 1/gamma-secretase-mediated epsilon-cleavage of N-cadherin, which results in the significant accumulation of N-cadherin C-terminal fragment 1 PMID: 21852538
    32. NCSTN and PSENEN, are involved in the pathogenesis of some familial hidradenitis suppurativa (Acne Inversa). PMID: 21412258
    33. structural analysis of PEN-2 conformation by single-particle electron microscopy PMID: 21454611
    34. This study support a gamma-secretase-independent role of presenilin-1 in modulation of filamin-mediated actin cytoskeleton. PMID: 20847418
    35. study found independent loss-of-function mutations in PSENEN, PSEN1, or NCSTN in 6 Chinese acne inversa (AI) families; results identify the gamma-secretase component genes as culprits for a subset of familial AI PMID: 20929727
    36. Data show that intramembranous cleavage by gamma-secretase and related intramembrane-cleaving proteases may generally occur via stepwise endoproteolysis. PMID: 20534834
    37. Hematopoietic gamma-secretase has reduced activity for APP and Notch1 processing compared to epithelial gamma-secretase. PMID: 20178366
    38. Transactivation of the Pen2 promoter by presenilin 1/2 is p53-dependent. PMID: 19889971
    39. a component of the gamma-secretase complex PMID: 12198112
    40. role in regulating proteolytic processing of presenilin 1 in conjunction with APH-1 PMID: 12522139
    41. examination of membrane topology PMID: 12639958
    42. APH-1 stabilizes the presenilin holoprotein in the complex, whereas PEN-2 is required for endoproteolytic processing of presenilin and conferring gamma-secretase activity to the complex. PMID: 12660785
    43. Expression of PEN2 increases amyloid beta peptide levels and gamma-secretase activity. PMID: 12763021
    44. presenilin 1 (PS1)-derived fragments, mature nicastrin, APH-1, and PEN-2, associate with cholesterol-rich detergent insoluble membrane (DIM) domains of non-neuronal cells and neurons PMID: 15322084
    45. the sequence and length of the C terminus of PEN-2 are critical for intermolecular interactions and function of presenilin complexes PMID: 15322109
    46. The presenilin-subunit stabilizing function of PEN-2 depends on length and overall sequence of its carboxyl-terminal domain. PMID: 15953349
    47. knockdown of ubiquilin-1 and -2 protein expression by RNAi (RNA interference) increased Pen-2 and nicastrin levels PMID: 15975090
    48. mutational analyses revealed that the "NF" sequence within the TMD4 of PS1 is the minimal motif that is required for binding with PEN-2, promoting PS1 endoproteolysis and gamma-secretase activity PMID: 16234243
    49. Pen-2 may contribute to the activation of the gamma-secretase complex by directly binding to the TMD4 of PS1 PMID: 16234244
    50. COX-2 may be a downstream effector of mutant N141I PS2-mediated apoptotic cell death and that inhibition of COX-2 may neuroprotect in AD through modulation of a GSK-3beta-beta-catenin-mediated response. PMID: 16331303

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  • 相关疾病：
    Acne inversa, familial, 2 (ACNINV2)
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    PEN-2 family
  • 组织特异性：
    Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
  • 数据库链接：

    HGNC: 30100

    OMIM: 607632

    KEGG: hsa:55851

    STRING: 9606.ENSP00000222266

    UniGene: Hs.534465