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  4. RECK Antibody

RECK Antibody

  • 货号：
    CSB-PA019536LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA019536LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA019536LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA019536LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The RECK antibody was produced in a rabbit with the recombinant human RECK protein (23-212aa) immunized. It reacts specifically with the human RECK protein. This RECK antibody was protein G-purified to at least 95% purity. Its specificity and reliability have been tested in ELISA, IHC, and IF applications.

    The RECK protein acts as a natural inhibitor of matrix metalloproteinases (MMPs), helping to prevent excessive extracellular matrix (ECM) breakdown and maintaining tissue integrity. RECK protein has also been implicated in other cellular processes, including cell migration, proliferation, and apoptosis. Dysregulation of RECK expression has been associated with various pathological conditions, including cancer, inflammation, and fibrosis.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) RECK Polyclonal antibody
  • Uniprot No.：
    O95980
  • 基因名：
    RECK
  • 别名：
    hRECK antibody; Membrane anchored glycoprotein (metastasis and invasion) antibody; RECK antibody; RECK protein antibody; RECK_HUMAN antibody; Reversion inducing cysteine rich protein with Kazal motifs antibody; Reversion-inducing cysteine-rich protein with Kazal motifs antibody; ST15 antibody; Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs) antibody; Suppressor of tumorigenicity 15 antibody; Suppressor of tumorigenicity 15 protein antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Reversion-inducing cysteine-rich protein with Kazal motifs protein (23-212AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，RECK Antibody (CSB-PA019536LA01HU)，的标记方式是Non-conjugated。对于RECK Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA019536LB01HU RECK Antibody, HRP conjugated ELISA
    FITC CSB-PA019536LC01HU RECK Antibody, FITC conjugated
    Biotin CSB-PA019536LD01HU RECK Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7. ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity. Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP).
  • 基因功能参考文献：
    1. Results report that the expression of RECK is significantly lower in cervical cancer cells than in normal cells. The relative protein expression levels of p53 signaling (p21 and Bax) were significantly elevated when RECK was upregulated, suggesting that the overexpression of RECK could promote the activation of p53 signaling pathway. PMID: 29064588
    2. overexpressed in trophoblasts from preeclampsia PMID: 29108633
    3. The expression of RECK was inhibited by the transfection of miR-96 mimics. RECK mRNA level was reduced by miR-96 mimics and increased by miR-96 inhibitor. In the invasion assay, miR-96 mimics were shown to promote tumor invasion. PMID: 29599320
    4. Results suggested that some paracrine substances produced by 786-0 cells may reduce RECK expression of adjacent HMEC-1 cells and enhance their proliferation and in vitro angiogenic capacity. PMID: 28561419
    5. RECK gene polymorphisms were closely associated with active proliferation, capsular invasion, and clinical recurrence of ameloblastoma. PMID: 28340422
    6. Collectively, these results demonstrated that IL-32alpha upregulates the atheroprotective genes Timp3 and Reck by downregulating microRNA-205 through regulation of the Rprd2-Dgcr8/Ddx5-Dicer1 biogenesis pathway. PMID: 28740544
    7. RECK CpG methylation pattern may predict prognosis and drug-sensitivity of breast cancers. PMID: 27058625
    8. The expression of RECK in human healthy and diseased gingiva may contribute to periodontal physiological and pathological processes; low RECK expression may be associated with the enhanced MMP-2 and MMP-9 production in inflamed gingiva. PMID: 28043014
    9. the RECK gene polymorphism influences molecular carcinogenesis and clinic pathological features of hepatocellular carcinoma within the Egyptian population PMID: 26921475
    10. Reversion-inducing, cysteine-rich protein with kazal motifs (RECK) was identified as the direct and functional target of miR-92b in osteosarcoma PMID: 26993249
    11. RECK expression in uterine leiomyoma is negatively regulated by miR-15b. PMID: 27530410
    12. RECK could regulate the expressions of MMP-2, 9 and MT1-MMP as a cell surface-signaling molecule. s propose that RECK may play an important role in regulating MMPs in the ECM degradation of periodontal diseases. PMID: 27272560
    13. Low expression of RECK is associated with oral cancer. PMID: 27696293
    14. RECK Gene Promoter rs10814325 Polymorphism is associated with metastasis in Hepatocellular Carcinoma PMID: 27268601
    15. the aim of this study was to analyze the effect of RECK gene rs 11788747 single nucleotide polymorphism (SNP) on hepatocellular carcinoma (HCC) susceptibility. PMID: 25278269
    16. Findings suggest that RECK transcript variants might have opposite roles in GBM biology and the ratio of their expression levels may be informative for the prognostic outcome of GBM patients. PMID: 26431549
    17. miR-21 has a role in upregulating PTEN, RECK and PDCD4 in glioma PMID: 26284486
    18. RECK is a regulator of hMSC functions suggesting that modulation of RECK may improve the development of hMSC-based therapeutical approaches in regenerative medicine. PMID: 26459448
    19. MMP-9/RECK imbalance in cervical smears is significantly associated with high-grade cervical diseases and infection by alpha-9 HPV and C. trachomatis. PMID: 26261088
    20. RECK is not an independent prognostic marker for breast cancer PMID: 26449734
    21. miR-221/miR-222-RECK axis might be an important path modulating H. pylori infection-related gastric cancer development PMID: 26364844
    22. Expression of RECK is associated with the incidence of restenosis after vascular angioplasty. RECK may be induced as a part of the intrinsic defense mechanism against restenosis. PMID: 26004948
    23. This downregulation of RECK in advanced grades of osteosarcoma and metastatic grades was also associated with the increased expression of invadosome-specific markers like MMP9 PMID: 26130413
    24. present data indicate that there is a significant association between mutant G of rs11788747 in RECK and WT risk PMID: 26141647
    25. D90 significantly inhibited the invasion and metastasis of OSCC cells by decreasing the expression of sp1 and increasing the expression of RECK to suppress the expression and activity of MMP-2 and MMP-9. PMID: 25877754
    26. Loss of RECK expression is associated with metastasis in Hepatoblastoma and Neuroblastoma. PMID: 25987077
    27. Notably, miR-21 regulated the potential anticancer effects of icariin on cell proliferation and apoptosis by targeting PTEN, RECK and Bcl-2 in the ovarian cancer A2780 cells PMID: 25845681
    28. RECK promoter activity is suppressed when RbAp46 binds to it and is involved in experimental lung metastasis. PMID: 25885317
    29. microRNA-200b and microRNA-200c promote colorectal cancer cell proliferation via targeting RECK. PMID: 25826661
    30. Association between RECK single nucleotide polymorphism and risk of hepatocellular carcinoma in the Han Chinese population PMID: 25412941
    31. The loss of E-cadherin expression is uncoupled from RECK-upregulation in carcinoma-derived cell lines. PMID: 24691523
    32. RECK was identified as a target of miR-96 and RECK overexpression abrogates the growth of esophageal cancer cells. PMID: 25465153
    33. demonstrate that RECK contributes to GA's anti-invasive activity and provide new evidence for GA being served as a therapeutic candidate for cancer metastasis PMID: 24532189
    34. Suggest that upregulation of miR-374b-5p contributes to gastric cancer cell metastasis and invasion through inhibition of RECK expression. PMID: 25516656
    35. no association between the RECK polymorphism and chemotherapy response status in non-small-cell lung cancer in Chinese cohort PMID: 24510537
    36. MiR-25 promotes cell proliferation by targeting RECK in human cervical carcinoma. PMID: 25575057
    37. A decrease in SPRY2 and RECK expression by nickel-induced miR-21 may promote invasiveness in lung cancer cells. PMID: 26026961
    38. These findings suggest that immunohistochemical staining for RECK could be useful in the differential diagnosis between CMM and BMN. PMID: 26026078
    39. RECK controls this angiogenic rheostat through a novel complex with cell surface receptors to regulate STAT3 activation PMID: 24931164
    40. these findings suggest that dysregulations of RECK and RAGE expressions may be collectively involved in tumor progression of nasopharyngeal carcinoma by regulating MMP9 expression PMID: 25031745
    41. There was weaker immunohistochemical expression of RECK protein in placental membranes of women with histologic chorioamnionitis compared to control subjects (P=0.0498). PMID: 24227542
    42. the data show that miR-200c expression sensitizes H460 cells to resveratrol and this is likely due to RECK expression. PMID: 24647918
    43. Our data suggest that miR-96 might promote the growth and motility of NSCLC cells partially by targeting RECK. PMID: 24469470
    44. Data indicate a consistent correlation between RECK, MT1MMP, and TIMP2 across different models of clinical samples and cell lines and suggest evidence of the potential use of this subset of genes as a gene signature for diagnosing melanoma. PMID: 24335752
    45. MiR-92b may promote nonsmall cell lung cancer cell growth and motility partially by inhibiting RECK. PMID: 24162673
    46. miR-96 promotes cellular proliferation, migration and invasion of MDA-MB-231 cells, at least in part, by targeting RECK. PMID: 24366472
    47. propose RECK down regulation in renal cell carcinoma to be an early event that facilitates tumor formation and progression PMID: 24131772
    48. miR-25 might promote gastric cancer cell growth and motility partially by targeting RECK. PMID: 24078004
    49. Data show that miR-221 is an oncogenic miRNA which may regulate colorectal cancer (CRC) migration and invasion through targeting RECK. PMID: 24269686
    50. Data indicate that reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) methylation was associated with clinical stage, histological differentiation and lymph node metastasis, but was not associated with gender, age, and tumor location. PMID: 23749490

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 组织特异性：
    Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
  • 数据库链接：

    HGNC: 11345

    OMIM: 605227

    KEGG: hsa:8434

    STRING: 9606.ENSP00000367202

    UniGene: Hs.388918