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  4. CD97 Recombinant Monoclonal Antibody

CD97 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA004972A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    The CD97 recombinant monoclonal antibody is a highly specific antibody against the human CD97 protein. This CD97 antibody was produced in vitro by transfecting human CD97 monoclonal antibody gene- vector clones into the cell line, followed by affinity-chromatography purification from the tissue culture supernatant (TCS). The mice were immunized with a human CD97 synthetic peptide to produce the human CD97 monoclonal antibody. This CD97 antibody has the same isotype as rabbit IgG. It can be applied in ELISA, IHC, and IF.

    CD97 is a neutrophil migratory factor that is widely expressed on hematopoietic cells. It was discovered to be a T-cell activation signal with minimal expression in naive T cells. CD97 has also been identified as a tumor-associated receptor since it is up-regulated in a variety of carcinomas, including gastric, colorectal, pancreatic, and other malignancies. These tumor cells' differentiation, proliferation, invasion, and metastasis are all linked to CD97. In the human immune system, CD97 and its ligand CD55 are significant binding partners. CD97-CD55 binding dysfunction has been associated with autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, and malignancies.

  • Uniprot No.：
    P48960
  • 基因名：
    CD97
  • 别名：
    ADGRE5; CD97; Adhesion G protein-coupled receptor E5; Leukocyte antigen CD97; CD antigen CD97
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3A7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
  • 基因功能参考文献：
    1. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities PMID: 27641734
    2. High CD97 expression Correlates with Breast, Colorectal and Pancreatic Cancer. PMID: 28373465
    3. High expression of CD97 is associated with lymphatic metastasis in gastric cancer. PMID: 26233326
    4. This study indicated that the CD97 and CD55 proteins might be reliable biomarkers to predict the metastasis status and prognosis of intrahepatic cholangiocarcinoma patients. PMID: 28345461
    5. Knock down of CD97 led to an altered mechanical phenotype, reduced adhesion to a stromal layer and lower wildtype FLT3 expression. PMID: 26462154
    6. present study suggested that the expressions of CD97 antigen and decay accelerating factor(DAF) were both upregulated in human cervical squamous cell carcinoma PMID: 26107567
    7. CD97 promotes gastric cancer cell proliferation and invasion in vitro through exosome-mediated MAPK signaling pathway, and exosomal miRNAs are probably involved in activation of the CD97-associated pathway. PMID: 26034356
    8. we identify the specific isoforms of CD97, a novel pro-invasive glioma antigen, across histologic grades of glioma and within BTICs. We also demonstrate a trend towards increased CD97 expression among the classical and mesenchymal GBM subtypes. PMID: 25714433
    9. We conclude that CD97 is located in the SR and at the peripheral sarcolemma of human and murine skeletal muscle, where its absence affects the structure of the SR without impairing skeletal muscle function PMID: 24949957
    10. CD97 enhanced TIMP-2 secretion, leading to reduced MT-MMP-1 and -2 activities, impairing cell migration/invasion in vitro and lung macrometastasis in vivo and upregulating integrins. Both the NTF and the CTF of CD97 were required. PMID: 25174588
    11. These results provide the first experimental evidence that cd97 is a direct target of miR-126. PMID: 24274104
    12. study reports gene expression in skeletal muscle tissue of women with metabolic syndrome is enriched in inflammatory response-related genes; IL6R, HDAC9 and CD97 expression correlated negatively with insulin sensitivity; suggests a role for these 3 inflammatory genes in development of skeletal muscle insulin resistance in women PMID: 23771909
    13. Lysophosphatidylethanolamine utilizes LPA(1) and CD97 in a breast cancer cell line. PMID: 23838008
    14. CD97 expression promotes invasion and migration in glioblastoma multiforme, but has no effect on tumor proliferation. PMID: 23658650
    15. CD97 expression in human thyroid cancers correlated with LPA receptor and markers of aggressiveness including Ki67 and pAKT. PMID: 22797060
    16. CD97 small isoform not only supported gastric cancer local growth, but also promoted metastatic spread in orthotopically implanted mouse model. PMID: 22768192
    17. CD97 and CD55 showed high expression at the invasive front of gallbladder carcinoma. CD97 and CD55 expression was associated with high histologic grade, advanced pathologic T stage, clinical stage and positive venous/lymphatic invasion. PMID: 22547928
    18. binding of leukocytes to activated endothelium mediated by the interaction of CD97 with Thy-1 is involved in firm adhesion of polymorphonuclear cells during inflammation and may play a role in the regulation of leukocyte trafficking to inflammatory sites. PMID: 22210915
    19. we conclude that the possible upregulation of CD97 mediated by WT1 promotes cellular invasiveness-one of the most characteristic and challenging aspects of glial tumor cells. PMID: 22313360
    20. CD97 functioned to mediate invasion in prostate cancer cells, by associating with lysophosphatidic acid receptor 1 (LPAR1), leading to enhanced LPA-dependent RHO and extracellular signal-regulated kinase activation. PMID: 21978933
    21. expression of the wild type - but not the GPS cleavage-deficient CD97 up-regulates the expression of N-cadherin, leading to Ca(++)-dependent cell-cell aggregation. PMID: 21156175
    22. Elevated expression of CD97 and its ligand CD55 at the invasion front correlate with tumor recurrence and metastasis, and CD95 may be a poor prognostic factor for rectal adenocarcinoma. PMID: 20339853
    23. the tumor promoting role of CD97 small isoform in cancer progression PMID: 20428763
    24. complex cellular expression programmes rather than activation modes regulate the expression of EGF-TM7 receptors in macrophages PMID: 20167235
    25. CD97 expression correlates with dedifferentiation, migration, and invasion in colorectal tumor cell lines PMID: 12414513
    26. coengagement of alpha5beta1 and chondroitotin sulfate proteoglycan by CD97 synergistically initiates endothelial cell invasion PMID: 15576472
    27. Findings suggest that CD97(EGF) may play a role in the development and invasion of gastric carcinomas. PMID: 16273233
    28. CD55 engagement with its natural ligand CD97 can act as a potent costimulator of human CD4+ T cells, resulting in cellular activation and promoting enhanced proliferation and cytokine secretion. PMID: 16818763
    29. enhanced CD97 expression in colorectal cancer cells is regulated independent of beta-catenin/Tcf-4, and is thus not a direct target of the canonical Wnt pathway PMID: 16929497
    30. CD55 may simultaneously regulate both the innate and adaptive immune responses and can also regulate complement when bound to CD97. PMID: 17449467
    31. EGF-TM7 pre-mRNAs also undergo the rare trans-splicing, leading to the generation of functional chimeric receptors. PMID: 18267122
    32. Sp1 and Sp3 over-expression activates CD97 promoter activity in HEK293 cells. PMID: 18329191
    33. CD97 is present on all lymphocytes in blood and lymphoid tissue. Expression of CD97 on B cells was lower compared to T and NK cells and did not differ between B-cell subsets. PMID: 19428565
    34. CD97-mFc can adopt two different conformations; one capable of auto-proteolysis and the other not. PMID: 19737555
    35. CD97 is expressed in all types of macrophages and dendritic cells except for microglia, in most T cells but only a few B cells, in smooth muscle cells, and in a restricted set of thyroid and gastrointestinal carcinomas. PMID: 11380941

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.; [Adhesion G protein-coupled receptor E5 subunit alpha]: Secreted, extracellular space.
  • 蛋白家族：
    G-protein coupled receptor 2 family, LN-TM7 subfamily
  • 组织特异性：
    Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and p
  • 数据库链接：

    HGNC: 1711

    OMIM: 601211

    KEGG: hsa:976

    STRING: 9606.ENSP00000242786

    UniGene: Hs.466039