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DDX6 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA180423A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA180423A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    DDX6 is a conserved DEAD-box protein (DBP) that plays central roles in cytoplasmic RNA regulation, including processing body (P-body) assembly, mRNA decapping, and translational repression. DDX6 has pro- and antiviral roles in viral infections. DDX6 modulates replication and the stability of HCV RNA through interaction with miR-122 and the viral-5′UTR. In mosquitoes, DDX6 plays a role against the flavivirus, such as West Nile virus and Zika virus. In HIV, DDX6 is implicated in negatively regulating viral replication.

    The production of this recombinant DDX6 antibody was carried out in vitro. It began with immunization of animals so that the B cells could be obtained. The next step was selection of B cells. The positive cells would be screened out for the next step, single B cell antibody sequencing and cloning. Once the DDX6 antibody sequence was obtained, it would be inserted into a plasmid, which could be transfected into mammalian cells for the expression of DDX6 antibody.

  • Uniprot No.：
    P26196
  • 基因名：
    DDX6
  • 别名：
    Probable ATP-dependent RNA helicase DDX6 (EC 3.6.4.13) (ATP-dependent RNA helicase p54) (DEAD box protein 6) (Oncogene RCK), DDX6, HLR2 RCK
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human DDX6
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6D8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
  • 基因功能参考文献：
    1. Results provide evidence for a dual mechanisms regulating the nucleocytoplasmic localization of DDX6. First, data show that DDX6 can be transported by 4E-T in a piggyback manner. Furthermore, a novel nuclear targeting mechanism was detected in which DDX6 enters the newly formed nuclei by "hitch-hiking" on mitotic chromosomes with its C-terminal domain during M phase progression. PMID: 28216671
    2. Results showed that DDX6 protein was overexpressed in gastric cancer (GC) and provided evidence that DDX6 acted as an upstream molecule that positively regulated the expression of HER2 and FGFR2 at the post-transcriptional step in GC cells. PMID: 29987267
    3. These findings imply a novel function for DDX6 as an RNA co-sensor and signaling enhancer for RIG-I. PMID: 29949917
    4. Identification of the DEAD box RNA helicase DDX6 as a new partner that acts in cooperation with Tau to increase miRNA activity. PMID: 29966762
    5. DDX6 modulates interaction of miR-122 with the 5' untranslated region of hepatitis C virus RNA. PMID: 28456022
    6. we demonstrate that joint deletion of two short conserved motifs that bind UNR and DDX6 relieves repression of 4E-T-bound mRNA, in part reliant on the 4E-T-DDX6-CNOT1 axis. PMID: 27342281
    7. miRNAs, AGOs, GW182, the CCR4-NOT complex, and DDX6/Me31B repress and degrade polyadenylated mRNA targets that are translated via scanning-independent mechanisms in both human and Drosophila melanogaster cells PMID: 27009120
    8. DDX6 may have a role in radio- and chemoresistance in glioblastoma PMID: 26783102
    9. Functional analysis of the 11q23.3 glioma susceptibility locus implicates PHLDB1 and DDX6 in glioma susceptibility. PMID: 26610392
    10. DDX6 repression complexes are required for P-body assembly. PMID: 25995375
    11. Protein Interactome Analysis of DDX6 PMID: 26184334
    12. Results indicate that progenitor function is maintained by DDX6 complexes through two distinct pathways that include the degradation of differentiation-inducing transcripts and by promoting the translation of self-renewal and proliferation mRNAs. PMID: 26412305
    13. CNOT1 facilitates recruitment of DDX6 to miRNA-targeted mRNAs, placing DDX6 as a downstream effector in the miRNA silencing pathway. PMID: 25035296
    14. study discovered multiple susceptibility variants for systemic lupus erythematosus in the 11q23.3 region, including variants in/near PHLDB1 (rs11603023), DDX6 (rs638893) and CXCR5 (rs10892301) PMID: 24001599
    15. Crystal structures of the DDX6, CNOT1 and CNOT9 complexes. PMID: 24768538
    16. Crystal structure of the DDX6, CNOT9 and CNOT1 complex. PMID: 24768540
    17. DDX6 and miR-122 modulate HCV through distinct pathways. PMID: 23826300
    18. data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping PMID: 23535175
    19. High DDX6 expression is associated with gastric cancer. PMID: 23932921
    20. We identified several unbalanced aberrations and a t(11;14) involving IGH and DDX6 providing evidence for a contribution of DDX6 to lymphomagenesis by deregulation of BCL6 in NMZL. PMID: 22965301
    21. DEAD-box RNA helicase 6 (DDX6) is a cellular modulator of vascular endothelial growth factor expression under hypoxia PMID: 23293030
    22. Rck/p54 recruitment by sequence-specific translational repressors leads to further binding of Rck/p54 along mRNA molecules, resulting in their masking, unwinding, and ultimately recruitment to P-bodies. PMID: 22836354
    23. assembling HIV-1 co-opts a preexisting host complex containing cellular facilitators such as DDX6, which the virus uses to catalyze capsid assembly PMID: 22851315
    24. Data show that DDX6 is required for efficient assembly or release of infectious Dengue virus. PMID: 21957497
    25. stud finds that DDX6 is required for efficient genome packaging of foamy virus, a spumaretrovirus PMID: 22022269
    26. RCK is a functional partner of TTP in promoting TTP-mediated translational repression of AU-rich mRNA PMID: 22203041
    27. data suggest that in premature erythroid cells translational silencing of hr15-LOX mRNA is maintained by DDX6 mediated storage in these RNP granules PMID: 20884783
    28. DDX6 helicase activity is essential for efficient hepatitis C virus replication, reflecting essential roles for DDX6 in hepatitis C virus genome amplification and/or maintenance of cellular homeostasis. PMID: 20392846
    29. In the Australian GWAS, one SNP achieved genomewide significance for comorbid AD/ND, rs1784300 near DDX6 on chromosome 11 (p = 2.60 x 10(-9)). PMID: 20158304
    30. DEAD-box helicase rck/p54, is involved in post-transcriptional regulation of APP, as its overexpression in cultured cells results in elevated levels of APP mRNA and protein PMID: 18378046
    31. Rck/p54 protein is possibly involved in the replication of HCV genomes in hepatocytes and in carcinogenesis of hepatocellular carcinoma. PMID: 12823589
    32. The Over-expression of RCK-p54 protein in HeLa cells caused growth inhibition and cell cycle arrest at G2/M with down-regulation of c-myc expression. PMID: 16611246
    33. Study shows that RCK/p54, a DEAD box helicase, interacts with Ago1 and Ago2, in active siRISC or miRISC from human cells; directly interacts with Ago1 and Ago2 in vivo, facilitates formation of P-bodies, and is a general repressor of translation. PMID: 16756390
    34. ataxin-2 interacts with the RNA helicase DDX6 PMID: 17392519
    35. RCK/ p54 might be a determinant of colorectal cancer proliferation by activating the canonical Wnt pathway PMID: 18769115
    36. Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B. PMID: 19285948
    37. The role of the p54 helicase activity in translational repression and in P-body formation, was examined. PMID: 19297524

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  • 相关疾病：
    A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).
  • 亚细胞定位：
    Cytoplasm, P-body. Cytoplasm. Nucleus.
  • 蛋白家族：
    DEAD box helicase family, DDX6/DHH1 subfamily
  • 组织特异性：
    Abundantly expressed in most tissues.
  • 数据库链接：

    HGNC: 2747

    OMIM: 600326

    KEGG: hsa:1656

    STRING: 9606.ENSP00000264018

    UniGene: Hs.408461