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PTH1R Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA162005A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: U-251 whole cell lysate, THP-1 whole cell lysate, Ntera-2 whole cell lysate
      All lanes: PTH1R antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 67 kDa
      Observed band size: 72, 55 kDa
  • 其他：

产品详情

  • 产品描述：

    PTH1R is widely expressed during early development but has its highest expression in bone and kidneys. Upon binding with either of its two ligands, PTH and PTHrP, PTH1R is able to activate the Gαs/adenylyl cyclase/PKA pathway, Gαq/phospholipase C/PKC pathway, Gα12/13-phospholipase D/RhoA pathway as well as MAPK signaling cascade. PTH1R signaling plays a pivotal role in the regulation of multiple physiological functions including mineral ion homeostasis, skeletal development, bone metabolism, tooth eruption, tooth root formation, alveolar bone regeneration, and periodontium repair.

    The generation of this recombinant PTH1R antibody occurs in a series of steps: immunization, splenocytes & PBMC, single B cell sorting, mRNA extraction, RT-PCR & insert vector, expression, ELISA validation. And ELISA, WB was carried out Every step was performed under strict standards to ensure the researchers can have a recombinant PTH1R antibody with premium quality.

  • Uniprot No.：
    Q03431
  • 基因名：
  • 别名：
    Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH/PTHrP type I receptor) (PTH/PTHr receptor) (Parathyroid hormone 1 receptor) (PTH1 receptor), PTH1R, PTHR PTHR1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Parathyroid Hormone Receptor 1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • 基因功能参考文献：
    1. These results pinpoint the ubiquitinated Lys residues in PTHR controlling MAPK signaling and cell proliferation and survival. PMID: 29444827
    2. findings confirm the expression of CaSR in human BM-derived MSCs and unravel a prominent role for the interplay between CaSR and PTH1R in regulating MSC fate and the choice of pathway for bone formation. PMID: 29915064
    3. the decreased expression of PTH1R may be the main cause of hypercalcemia in HCC. Decreased expression of PTH1R was associated with tumor size, Edmondson Grade, serum AFP level and poor overall survival, and was a poor prognosis in HCC. PMID: 29278884
    4. the results indicated that the beta-alanine induced expression of PTHR1 has a positive relationship with invasion and metastasis of osteosarcoma cells. PMID: 29366883
    5. Functional studies (IFA) showed a variation in expression between the WT and mutant PTH1R. In silico analysis showed structural differences between WT and mutant PTH1R proteins, particularly in the regions of the 3rd intracellular loop and the 6th transmembrane domain required for efficient PTH1R function. PMID: 28643929
    6. Heterozygous mutations in the ATP4A and PTH1R genes were identified in a family with type I gastric neuroendocrine tumors plus hypothyroidism and rheumatoid arthritis PMID: 28474257
    7. It seems likely that Mut-PTH1R may be, at least in part, co-localized with Wt-PTH1R by forming a heterodimer, leading to affect the function to each other in Jansen type metaphyseal chondrodysplasia. PMID: 27160269
    8. PTH1R mutation is associated with Primary Failure of Tooth Eruption. PMID: 27898723
    9. Data suggest that GCGR (glucagon receptor) activation proceeds via a mechanism in which transmembrane helix 6 (TM6) is held in an inactive conformation by a conserved polar core and a hydrophobic lock (involving intracellular loop 3, IC3); mutations in the corresponding polar core of GCGR or PTH1R disrupt these inhibitory elements, allow TM6 to swing outward, and induce constitutive G protein signaling. PMID: 28356352
    10. data highlight sequences in PTHR that contribute to NHERF1 interaction and can be altered to prevent phosphorylation-mediated inhibition PMID: 28376304
    11. Overt hypercalcemia is not always encountered in Jansen Metaphyseal Chondrodysplasia due to Heterozygous H223R-PTH1R Mutation. PMID: 27410178
    12. data are consistent with the hypothesis that the pattern of C-terminal tail phosphorylation on PTH1R may determine the signaling outcome following receptor activation. PMID: 27623777
    13. the data presented in this manuscript demonstrate a critical function of VPS35 in regulating PTH1R trafficking. This event and VPS35-interaction with PPP1R14C appear to be essential for turning off PTH1R's endosomal signaling, and promoting PTH1R-mediated catabolic response and bone remodeling. PMID: 27333042
    14. PTH1R mutation causing primary failure of tooth eruption in a consanguineous Saudi family. PMID: 27019138
    15. A critical role for SNX27-retromer mediated transport of PTHR in normal bone development. PMID: 26912788
    16. PI3K/Akt pathway stimulates the expressions of RANKL, PTHrP, and BMP-2 partly through NF-kappaB, suggesting its importance for bone metastasis of prostate carcinoma PMID: 27040945
    17. CaSR and PTH1R signaling responses in cartilage and bone. [review] PMID: 26688334
    18. PTHR recycles rapidly through at least two pathways, one involving the ASRT complex of actin, SNX27, and retromer and another possibly involving N-ethylmaleimide-sensitive factor. PMID: 27008860
    19. Data suggest affinity of ligands for binding site on PTHR1, either in GTP-binding protein-dependent or -independent conformation, alters duration of action of ligand in target cells; ligands were fragments of PTHRP/parathyroid hormone-related protein. PMID: 26562265
    20. This Review discusses current understanding of PTHR1 modes of action and how these findings might be applied in future therapeutic agents--{REVIEW} PMID: 26303600
    21. We show that sustained stimulation with PTH leads to diminished potentiation of carbachol-evoked Ca2+ signals. This does not require internalization of PTH1R. PMID: 25431134
    22. data indicate that, albeit the similarity in its subcellular distribution, PTH1R in PDL cells exhibits characteristics different from those in MG63 cells, pointing to the cell type specificity of this receptor PMID: 23604700
    23. data significantly increase the number of presently known unique PFE-causing PTH1R mutations and provide a series of variants with unclear pathogenicity which will require further in vitro assaying to determine effects on protein structure and function PMID: 23771181
    24. PTHR1 signaling is important in maintaining osteosarcoma proliferation and undifferentiated state. PMID: 25043296
    25. Treatment of recipient HEK 293a cells transiently expressing PTH1R with PTH-myc CM allowed the labeling of endosomal structures positive for Rab5 and/or for beta-arrestin1. PMID: 25128082
    26. evaluation of clinical and radiographic characteristics can heighten the specificity of ruling out suspected PTHR1 involvement in PFE patients PMID: 24825834
    27. PTHR concentrations are higher in patients with renal failure; the ratio between oxidized (ox)PTH and non-oxPTH varies substantially in renal failure patients; children have the highest mean as well as maximum n-oxPTH concentrations compared to adults. PMID: 23868100
    28. The PTH1R gene was analyzed in six patients clinically diagnosed with primary failure of tooth eruption. PMID: 24058597
    29. autosomal dominant mutations of PTH1R that cause PFE may also be associated with osteoarthritis; dose-dependent model may explain isolated PFE and osteoarthritis in absence of other known symptoms in the skeletal system. PMID: 24300310
    30. LCPUFAs, EPA and DHA, can activate PTH1R receptor at nanomolar concentrations and consequently provide a putative molecular mechanism for the action of fatty acids in bone PMID: 23300710
    31. beta-catenin binds to the PTHR-1 C-tail and switches the downstream signaling pathway from G(alphas)/cAMP to G(alphaq)/Ca(2+), which is a mechanism by which chondrocyte hypertrophy may be regulated through the PTH/PTHrP signal independent of canonical Wnt pathway PMID: 23124878
    32. PTHR forms a ternary complex that includes arrestin and Gbetagamma dimer in response to PTH stimulation, which in turn causes an accelerated rate of G(S) activation and increases the steady-state level of activated G(S), leading to prolonged generation of cAMP. PMID: 23297229
    33. [review] The PTH1R carboxy-terminal tail directs interactions with a plethora of binding partners, resulting in the activation of many pathways. PMID: 21777186
    34. Elevated levels of PTH1R expression were associated with breast cancer patients with diabetes. PMID: 21312071
    35. PTH(1-34) promotes coupled PTHR ubiquitination and deubiquitination, whereas PTH(7-34) activates only ubiquitination, thereby leading to PTHR downregulation. PMID: 21898592
    36. Constitutive expression of PTHrP receptor type 1 in human bone marrow stromal cells declines with age. PMID: 21518242
    37. PTH-receptors regulate norepinephrine release in human heart and kidney PMID: 21756942
    38. Dynamic Na+-H+ exchanger regulatory factor-1 association and dissociation regulate parathyroid hormone receptor trafficking at membrane microdomains PMID: 21832055
    39. Ezrin promotes PTH1R mediated signaling via phospholipase and PIP2 depletion impedes receptor cell surface expression in HEK293 cells. PMID: 21672629
    40. A novel variant of parathyroid hormone 1 receptor gene (PTH1R), R383Q, was cosegregated in the first primary failure of tooth eruption family. PMID: 21404329
    41. Study shows that binding to beta-arrestin1 prolongs rather than terminates the generation of cAMP by PTHR, and that cAMP generation correlates with the persistence of arrestin-receptor complexes on endosomes. PMID: 21445058
    42. Its genetic defect leads to chondrodysplasia. (review) PMID: 20890029
    43. NHERF1 may serve as an adaptor, bringing beta-arrestin2 into close proximity to the PTHR, thereby facilitating beta-arrestin2 recruitment after receptor activation. PMID: 20656684
    44. Vascular smooth muscle PTH1R activity inhibits arteriosclerotic Wnt/beta-catenin signaling and reduces vascular oxidative stress, thus limiting aortic type I collagen and calcium accrual in diabetic LDLR-deficient mice. PMID: 20489161
    45. In both central and peripheral giant cell granulomas of the jaws, PTHR1 was abundantly expressed by type I multinucleated giant cells and mononucleated stromal cells with vesicular nuclei. PMID: 20060342
    46. The crystal structure of the ligand-free PTH1R extracellular domain (ECD) reveals a dimer in which the C-terminal segment of both ECD protomers forms an alpha-helix that mimics PTH/PTHrP by occupying the peptide binding groove of the opposing protomer. PMID: 20172855
    47. TGFBR2 forms an endocytic complex with PTH1R in response to PTH and regulates signalling by PTH and TGF-beta. TGFBR2 directly phosphorylates the PTH1R cytoplasmic domain, which modulates PTH-induced endocytosis of the PTH1R-TGFBR2 complex. PMID: 20139972
    48. Agonist-regulated cleavage of the extracellular domain of parathyroid hormone receptor type 1 PMID: 20080964
    49. A PTH1R mutation is strongly associated with failure of orthodontically assisted eruption or tooth movement; specific treatments are discussed. PMID: 20152661
    50. different domains of PTHR implicated in agonist-dependent receptor internalization; the receptor's core (Asn-289 and Lys-382) appears to regulate internalization of the receptor/beta-arrestin complex toward early endocytic endosomes during endocytosis. PMID: 11726668

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  • 相关疾病：
    Jansen metaphyseal chondrodysplasia (JMC); Chondrodysplasia Blomstrand type (BOCD); Enchondromatosis multiple (ENCHOM); Eiken skeletal dysplasia (EISD); Primary failure of tooth eruption (PFE)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 2 family
  • 组织特异性：
    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • 数据库链接：

    HGNC: 9608

    OMIM: 125350

    KEGG: hsa:5745

    STRING: 9606.ENSP00000321999

    UniGene: Hs.1019