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  4. Recombinant Epstein-Barr virus Latent membrane protein 2 (LMP2), partial

Recombinant Epstein-Barr virus Latent membrane protein 2 (LMP2), partial

In Stock
  • 货号：
    CSB-EP321086EFA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    LMP2
  • Uniprot No.：
  • 别名：
    LMP2; Latent membrane protein 2; Terminal protein
  • 种属：
    Epstein-Barr virus
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    31.6kDa
  • 表达区域：
    1-147aa
  • 氨基酸序列
    MGSLEMVPMGAGPPSPGGDPDGYDGGNNSQYPSASGSSGNTPTPPNDEERESNEEPPPPYEDPYWGNGDRHSDYQPLGTQDQSLYLGLQHDGNDGLPPPPYSPRDDSSQHIYEEAGRGSMNPVCLPVIVAPYLFWLAAIAASCFTAS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Isoform LMP2A maintains EBV latent infection of B-lymphocyte, by preventing lytic reactivation of the virus in response to surface immunoglobulin (sIg) cross-linking. Acts like a dominant negative inhibitor of the sIg-associated protein tyrosine kinases, LYN and SYK. Also blocks translocation of the B-cell antigen receptor (BCR) into lipid rafts, preventing the subsequent signaling and accelerated internalization of the BCR upon BCR cross-linking. Serves as a molecular scaffold to recruit SYK, LYN and E3 protein-ubiquitin ligases, such as ITCH and NEDD4L, leading to ubiquitination and potential degradation of both tyrosines kinases. Possesses a constitutive signaling activity in non-transformed cells, inducing bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin-negative cells to colonize peripheral lymphoid organs.; Isoform LMP2B may be a negative regulator of isoform LMP2A.
  • 基因功能参考文献：
    1. In EBV-positive Hodgkin's lymphoma LMP2A-expressing HRS cells lacking BCR signalling functions cannot induce EGR1 and are consequently protected from entry to the virus lytic cycle. PMID: 23592216
    2. Epstein-Barr virus latent membrane protein 2A contributes to anoikis resistance through ERK activation. PMID: 23698301
    3. LMP2A, but not LMP2B, is critical for efficient long-term growth of B cells in vitro. PMID: 23308294
    4. LMP2A plays a role in the alteration of a cytokine that is important for both tumour survival and anti-tumour responses. PMID: 23303827
    5. LMP2A can affect a number of signaling pathways by regulating the phosphorylation of the ITSN1 and Shb adaptors. PMID: 22975684
    6. Data show that LMP2A enhances antigen presentation function. PMID: 22616025
    7. hnRNP K, when co-expressed with EBNA2, strongly enhances viral latent membrane protein 2A (LMP2A) expression. PMID: 22879910
    8. Epstein-Barr virus latent membrane protein-2A induces ITAM/Syk- and Akt-dependent epithelial migration through alphav-integrin membrane translocation PMID: 22837212
    9. Fra-1 was induced by Epstein-Barr virus LMP2A and is essential for LMP2A-triggered MMP9 expression. PMID: 22514348
    10. Widespread sequence variations in the LMP2A gene were found PMID: 22470549
    11. Studies suggest that LMP2A can also participate in EBV-induced epithelial cell growth transformation. PMID: 22249143
    12. EBV LMP2A is expressed in nasopharyngeal cancer. Cases with nasopharyngeal inflammation showed negative expression of LMP2A. PMID: 16124641
    13. Results confirmed that HPV L1 protein is potential to deliver multiepitope of EBV LMP2 as immunogen. PMID: 20705592
    14. LMP2A induces an epithelial-mesenchymal transition and increases the number of side population stem-like cancer cells in nasopharyngeal carcinoma. PMID: 20532215
    15. LMP2A uniquely makes resting B-cells sensitive to NF-kappaB inhibition and apoptosis and suggest that NF-kappaB may be a novel target to eradicate latently EBV-infected B-cells. PMID: 20484564
    16. LMP2A induces a heightened sensitivity to TLR ligand stimulation PMID: 16920914
    17. Expression of the EBV genes for latent membrane protein 1 and latent membrane protein 2A decreases FoxO1 expression.[LMP-2A] PMID: 16943287
    18. Phosphorylation of LMP2A is prevented by LMP2B. PMID: 17035319
    19. results indicate that cholesterol-dependent LMP2A trafficking determines the fate of LMP2A degradation PMID: 17150237
    20. The signalling scaffold Shb associates through SH2 and PTB domain interactions with phosphorylated tyrosine motifs in the LMP2A N-terminal tail. PMID: 17311000
    21. These results suggest that constitutive activation of the Ras/PI3-K/Akt pathway by LMP2A is a key factor for LMP2A-mediated transformation. PMID: 17582000
    22. All isolates from 7 Japanese nasal NK/T cell lymphomas showed the same amino acid change from serine to threonine at codon 348 in the CTL epitope SSCSSCPLSK of LMP2A. PMID: 17657160
    23. A role for LMP2A as an indispensable BCR mimic in certain B cells from which human B-cell tumors such as Hodgkin lymphoma originate. PMID: 17682125
    24. The results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2A promoter. PMID: 17898065
    25. These results demonstrate that LMP2A activates the Notch pathway in both B cells and epithelial cells, and exploits this pathway to regulate its own expresison. PMID: 17980397
    26. EBV uses its latent protein, LMP2A, to activate the NF-kappaB-survivin pathway to rescue EBV-infected epithelial cells from serum deprivation PMID: 18316606
    27. Despite the lack of pre-BCR expression, bone marrow B cells from TgE LMP2A mice proliferate and survive in low concentrations of interleukin 7, similar to wild-type cells. PMID: 18559925
    28. This demonstrates that the UGDH transcript and protein quantities, the enzyme activity, and glycosaminoglycan contents increase in LMP2A overexpressed human embryonic kidney 293 (HEK293) cells. PMID: 18717819
    29. LMP2A and the Notch1 pathway may cooperate to induce the alterations in B-cell identity seen in Hodgkin Reed-Sternberg cells PMID: 18815281
    30. LMP2B modulates the activity of LMP2A contributing to maintenance of Epstein-Barr virus latency. PMID: 18835714
    31. the immunoreceptor tyrosine-based activation motif (ITAM) of LMP-2A is important for HERV-K18 env transactivation PMID: 19070345
    32. c-Cbl promoted LMP2A degradation through ubiquitination, specifically degraded the Syk protein tyrosine kinase in the presence of LMP2A, and inhibited LMP2A induction of the EBV lytic cycle PMID: 19081591
    33. EBV LMP2A promotes tumor development by protecting pre-tumor B cells that would normally apoptose after the c-myc translocation. PMID: 19182823
    34. LMP2A can permit tumorigenesis in the presence of an intact p53 pathway, identifying an important contribution of EBV to Burkitt's lymphoma PMID: 19815507
    35. Our findings emphasize the role of miR-BART22 in modulating LMP2A expression PMID: 19881953

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  • 亚细胞定位：
    [Isoform LMP2A]: Host cell membrane; Multi-pass membrane protein.; [Isoform LMP2B]: Host endomembrane system; Multi-pass membrane protein. Host cytoplasm, host perinuclear region.
  • 蛋白家族：
    Herpesviridae LMP-2 family
  • 数据库链接：

    KEGG: vg:3783751