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  4. Recombinant Human Glutamate decarboxylase 1 (GAD1)

Recombinant Human Glutamate decarboxylase 1 (GAD1)

  • 货号：
    CSB-YP858702HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP858702HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP858702HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP858702HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP858702HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GAD1
  • Uniprot No.：
  • 别名：
    67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; DCE1_HUMAN; EC 4.1.1.15; FLJ45882; GAD 1; GAD 67; GAD; GAD-67; GAD1; Glutamate decarboxylase 1 (brain; 67kDa); Glutamate decarboxylase 1; Glutamate decarboxylase 1 brain 67kD; Glutamate decarboxylase 1 brain 67kDa; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase; brain; 67-KD; OTTHUMP00000041054; OTTHUMP00000041055; OTTHUMP00000228572; SCP
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-594
  • 氨基酸序列
    MASSTPSSSA TSSNAGADPN TTNLRPTTYD TWCGVAHGCT RKLGLKICGF LQRTNSLEEK SRLVSAFKER QSSKNLLSCE NSDRDARFRR TETDFSNLFA RDLLPAKNGE EQTVQFLLEV VDILLNYVRK TFDRSTKVLD FHHPHQLLEG MEGFNLELSD HPESLEQILV DCRDTLKYGV RTGHPRFFNQ LSTGLDIIGL AGEWLTSTAN TNMFTYEIAP VFVLMEQITL KKMREIVGWS SKDGDGIFSP GGAISNMYSI MAARYKYFPE VKTKGMAAVP KLVLFTSEQS HYSIKKAGAA LGFGTDNVIL IKCNERGKII PADFEAKILE AKQKGYVPFY VNATAGTTVY GAFDPIQEIA DICEKYNLWL HVDAAWGGGL LMSRKHRHKL NGIERANSVT WNPHKMMGVL LQCSAILVKE KGILQGCNQM CAGYLFQPDK QYDVSYDTGD KAIQCGRHVD IFKFWLMWKA KGTVGFENQI NKCLELAEYL YAKIKNREEF EMVFNGEPEH TNVCFWYIPQ SLRGVPDSPQ RREKLHKVAP KIKALMMESG TTMVGYQPQG DKANFFRMVI SNPAATQSDI DFLIEEIERL GQDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the production of GABA.
  • 基因功能参考文献：
    1. Association between GAD1 rs3749034 polymorphisms with antisaccade task performance in schizophrenic patients. PMID: 29429137
    2. This study detected significant reductions in the mRNAs associated with GAD1 in the frontal cortex (FC) of autism spectrum disorder. PMID: 28229923
    3. Our results demonstrate a weak effect of the GAD1 gene on the risk of bipolar illness, and the associated marker might represent a proxy for real signals of rare variants. PMID: 28710909
    4. findings converge on variation in glutamic acid decarboxylase 1 gene predicting a susceptibility mechanism that affects white matter fractional anisotropy, GABAergic inhibitory neurotransmission in the dorsolateral prefrontal cortex, and working memory performance. PMID: 26822489
    5. Results show how epigenetic changes in GAD1 expression alter local glutamate metabolism in the brain metastatic microenvironment, contributing to a metabolic adaption that facilitates metastasis outgrowth in that setting. PMID: 28400476
    6. We investigate the possible influence of GAD1 SNPs rs3749034 and rs11542313 on ADHD susceptibility. The C allele of rs11542313 was significantly overtransmitted from parents to ADHD probands. Hyperactive/impulsive score was higher in rs3749034G allele and rs11542313C allele carriers. GAD1 haplotypes were associated with higher hyperactive/impulsive scores in ADHD youths. GAD1 gene is associated with ADHD susceptibility. PMID: 27530595
    7. GAD1 is reactivated by DNA methylation, which provided a model for DNA methylation and the active orchestration of oncogenic gene expression by CTCF in cancer cells. PMID: 26549033
    8. There was no difference in GAD25 and GAD67 gene expression level, and GAD25/GAD67 ratio between patients with first episode psychosis and healthy controls PMID: 28122016
    9. Genetic variability in GAD1 and GAD2 contributes to the risk of methamphetamine dependence in the Thai population. PMID: 27967329
    10. Reduced GAD67 expression in PV neurons is not an upstream cause of the lower levels of GABA-associated transcripts, or of the characteristic behaviors, in schizophrenia. PMID: 26980143
    11. Glutamic acid decarboxylase 67 (GAD67) is one of the isoforms that catalyze GABA synthesis. Here, we used recombinant herpes simplex virus (HSV-1) vectors that encode gad1 gene to evaluate the therapeutic potential of GAD67 in peripheral HIV gp120-induced neuropathic pain PMID: 26752351
    12. This study showed that in female groups, the expression of GABRA5 was generally higher in schizophrenia cases compared to the control. PMID: 25660468
    13. GAD autoantibodies were associated with risk of developing type 1 diabetes. PMID: 25523979
    14. Conversion of GAD autoantibody is associated with patients with presumed type 2 diabetes. PMID: 25301693
    15. The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells. PMID: 25647668
    16. These results suggest a link between altered inhibitory mechanisms and synchrony and, therefore point to potential mechanisms via which a disruption in neurodevelopmental processes might lead to pathophysiology associated with schizophrenia. PMID: 25072323
    17. Gene ransfer of GAD67 by herpes simplex virus vectors prevents HIV gp120/ddC-induced neuropathic pain. PMID: 25851180
    18. Phosphate-activated glutaminase and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls PMID: 24950944
    19. results statistically show that a reduction in GAD1 and SCL1A2 expression in the dorsolateral prefrontal cortex in subjects with major depressive disorder is related to a possible attenuated RAF/MEK/ERK pathway PMID: 24652383
    20. CNR1, GAD1 and BDNF polymorphisms are associated with male heroin dependence in the Dai population in China. PMID: 25252306
    21. The decreased amount of GAD65 and GAD67 suggests the decreased synthesis of neurotransmitter and basic GABA pools that indicates insufficient functioning of the GABA system in the cerebellar cortex of Alzheimer's disease patients. PMID: 24874321
    22. Deficient Zif268 mRNA expression may contribute to lower cortical GAD67 levels in schizophrenia, suggesting altered cortical GABA synthesis and impaired cognition in schizophrenia. PMID: 24874453
    23. A compensatory role was found for the GAD1 gene hypomethylation in panic disorder depending on genetic variation. PMID: 23906988
    24. GAD1 expression correlates with cellular invasiveness and migration by regulating beta-catenin translocation and MMP7 activation. PMID: 24261884
    25. The data of this study showed a robust reduction of cortical thickness in the left parahippocampal gyrus (PHG) in G allele homozygotes of GAD1 rs3749034. PMID: 23566421
    26. The GG GAD1 haplotype (rs3791862, rs16858977) frequency demonstrated significantly statistical difference between cerebral palsy patients and controls. PMID: 24228494
    27. Single nucleotide polymorphism(SNP)s in the GAD1 gene are associated with increased susceptibility to post-traumatic seizures after traumatic brain injury. PMID: 22840783
    28. In schizophrenia, abnormal PSA-NCAM and GAD67 expression may underlie the alterations observed in inhibitory neurotransmission. PMID: 23022470
    29. The present study represents the first systematic evaluation of gender-specific enrichment of risk alleles of the common SNP variation in the panic disorder candidate gene GAD1. PMID: 22662185
    30. This study provides further support for involvement of mainly GAD1, but also NPY in determining predisposition to anxiety disorders. PMID: 22328461
    31. Changes in copy number intensities vary in accordance with the disturbances in the expression of mRNAs associated with GAD67 regulation, but the patterns seen are fundamentally different for patients with schizophrenia and patients with bipolar disorder. PMID: 22309971
    32. Anti-idiotypic antibodies specific to GAD65Ab have a protective role in type 1 diabetes PMID: 22384267
    33. These findings point to a role for GAD1 polymorphism in heroin dependence among Han Chinese, and may be informative for future genetic or neurobiological studies on heroin dependence. PMID: 22564729
    34. Glutamate decarboxylase 67 is expressed in hippocampal mossy fibers of temporal lobe epilepsy patients PMID: 21509853
    35. The expression of synaptophysin and GAD67 was downregulated in the basolateral nucleus of schizophrenics. PMID: 22099865
    36. From midgestation to infancy, the pattern of GABAA receptor binding changed from uniformly low across all cortical layers to high levels concentrated in the middle laminae;GAD67 levels differentially increased. PMID: 21937910
    37. Support for the hypothesis that lower GABA synthesis in parvalbumin neurons contributes to dorsolateral prefrontal cortex dysfunction and impaired cognition in schizophrenia. PMID: 21632647
    38. Our findings show abnormal BDNF, trkB-TK+ and GAD67 mRNA expression in the hippocampus of individuals with schizophrenia and mood disorders. PMID: 21223646
    39. GAD65 is the major autoantigen in type 1 diabetes, and GAD67 per se has very low immunogenicity. PMID: 21494613
    40. Alterations of glutamic acid decarboxylase (GAD) play a crucial role in schizophrenic pathology. Read More: http://informahealthcare.com/doi/full/10.3109/15622975.2010.539270 PMID: 21250934
    41. Observations suggest that genetic variation in the GAD1 gene is unlikely to play a critical role in pervasive child development disorders. PMID: 21302352
    42. The correlation of GAD67 gene expression with MUC5AC expressions suggests that the up-regulation of airway epithelium expression of GAD67 may contribute to the increase in mucus production observed in association with cigarette smoking. PMID: 21034448
    43. some of Japanese patients who presented with acute onset diabetic ketosis and negative for GADAb share several clinical characteristics with atypical type 2 diabetes such as ketosis-prone diabetes and "soft-drink ketosis," but others do not PMID: 20960264
    44. results highlight a statistical association between one SNP of GAD67 (rs 11542313) and the condition of alcohol dependence. PMID: 20598486
    45. Analysis of expression data from a microarray study performed in samples from Brodmann area 9 confirms that GAD67 expression is decreased in Parkinson's disease PMID: 20832408
    46. These results support the importance of genetic variation in GAD1 in regulating prefrontal cortical GABA function. PMID: 20357758
    47. The overall amount of GAD-67 was significantly reduced (-34%) in depressed subjects compared to matched controls. PMID: 20236554
    48. Subjects with schizophrenia showed a significant, on average 8-fold deficit in repressive chromatin-associated DNA methylation at the GAD1 promoter. PMID: 17726539
    49. GAD gene transfer into glutamatergic excitatory neurons leading to an inhibitory bias with altered network activity and a neuroprotective phenotype holds potential for treatment of Parkinson's disease PMID: 12376704
    50. autoreactivity to GAD25 is rare in newly diagnosed type 1 diabetes and does not underlie GAD67 reactivity PMID: 12515288

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  • 相关疾病：
    Cerebral palsy, spastic quadriplegic 1 (CPSQ1)
  • 蛋白家族：
    Group II decarboxylase family
  • 组织特异性：
    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • 数据库链接：

    HGNC: 4092

    OMIM: 603513

    KEGG: hsa:2571

    STRING: 9606.ENSP00000350928

    UniGene: Hs.420036