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  4. Recombinant Human Hepatocyte nuclear factor 3-beta (FOXA2)

Recombinant Human Hepatocyte nuclear factor 3-beta (FOXA2)

  • 货号：
    CSB-YP897462HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP897462HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP897462HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP897462HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP897462HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Forkhead box A2; Forkhead box protein A2; FOX A2; foxa2; FOXA2_HUMAN; Hepatic nuclear factor 3 beta; Hepatocyte nuclear factor 3; Hepatocyte nuclear factor 3 beta; Hepatocyte nuclear factor 3-beta; HNF 3B; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF 3B; TCF-3B; TCF3B; Transcription factor 3B
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-457
  • 氨基酸序列
    MLGAVKMEGH EPSDWSSYYA EPEGYSSVSN MNAGLGMNGM NTYMSMSAAA MGSGSGNMSA GSMNMSSYVG AGMSPSLAGM SPGAGAMAGM GGSAGAAGVA GMGPHLSPSL SPLGGQAAGA MGGLAPYANM NSMSPMYGQA GLSRARDPKT YRRSYTHAKP PYSYISLITM AIQQSPNKML TLSEIYQWIM DLFPFYRQNQ QRWQNSIRHS LSFNDCFLKV PRSPDKPGKG SFWTLHPDSG NMFENGCYLR RQKRFKCEKQ LALKEAAGAA GSGKKAAAGA QASQAQLGEA AGPASETPAG TESPHSSASP CQEHKRGGLG ELKGTPAAAL SPPEPAPSPG QQQQAAAHLL GPPHHPGLPP EAHLKPEHHY AFNHPFSINN LMSSEQQHHH SHHHHQPHKM DLKAYEQVMH YPGYGSPMPG SLAMGPVTNK TGLDASPLAA DTSYYQGVYS RPIMNSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • 基因功能参考文献：
    1. might be a progesterone-induced gene, which may participate in the 'metastatic' process of eutopic endometrium to ectopic loci in patients with endometriosis PMID: 29233503
    2. FOXA2 may act as a tumor suppressor and inhibit the epithelial-mesenchymal transition of endometrial cancer cells. PMID: 29442045
    3. Our findings suggest that autophagy, through participation of FOXA2, maintains the characteristics of OCSCs. Autophagy and FOXA2 are therefore potential targets for ovarian cancer stem cell directed therapies. PMID: 29187221
    4. MAPK and FoxA2 mediate CS-induced squamous metaplasia. MAPK inhibitors upregulate FoxA2, resulting in a reduction in the degree of squamous metaplasia. PMID: 29200841
    5. In conclusion, our data suggest that overexpression of the Hedgehog components SHH, GLI2 and FOXA2 might be used as markers of an aggressive hemangioma. PMID: 28370639
    6. Our results suggest that FoxA2 plays a key role in the expression of hepatic phenotype of AFP-producing adenocarcinomas. PMID: 28778497
    7. FOXA2 is a specific and sensitive molecular marker for small cell neuroendocrine carcinoma of the prostate. PMID: 28621319
    8. HNF3beta plays a vital role in regulation of CPS1 gene and could promote the metabolism of ammonia by regulating CPS1 expression. PMID: 28272778
    9. The c.505T>C, p.S169P genetic variant occurs at the conserved forkhead DNA binding domain of the FOXA2 and causes a rare and unique clinical phenotype of hypopituitarism, CHI, dysmorphic features, liver, pancreas, heart and gastrointestinal abnormalities. PMID: 28973288
    10. Results showed that FOXA2 expression was decreased during Il-13 induced goblet cell differentiation but DNA methylation of its promoter was not changed. In the absence of Il-13, no difference was found for FOXA2 expression but hypomethylation of CpG # 10 and 11 in the its promoter was detected. These findings suggest that aberrant DNA methylation of FOXA2 is one of the factors underlying mucus hypersecretion in COPD. PMID: 28450970
    11. the findings of the current study provide compelling genetic evidence that FOXA2 is a pathogenic driver gene in the etiology of primary uterine cancers, including uterine carcinosarcomas and carcinomas. PMID: 28940304
    12. Physical and functional interactions between LXRalpha and FOXA2 were established in vitro and ex vivo. PMID: 28576574
    13. SIRT1 directly interacts with and deacetylates Foxa2 to inhibit its transcriptional activity on expression of genes involved in bile acids synthesis and transport. PMID: 27816442
    14. Iotansulin decreased LPL mRNA levels in HepG2 cells and this was associated with phosphorylation of AKT and nuclear export of FOXA2. PMID: 28126606
    15. findings provide the structural basis for FOXA2 protein binding to a consensus forkhead site and elucidate how members of the forkhead protein family bind different DNA sites. PMID: 28644006
    16. Low FOXA2 expression is associated with metastasis of gastric cancer. PMID: 28098868
    17. FOXA2 identified as a novel tumor suppressor in pancreatic cancer. It is regulated directly by miR-199a. PMID: 27151939
    18. This study used combined immunohistochemical staining for a marker of hepatic commitment and specification (FOXA2 [Forkhead box A2]), hepatocyte maturation (Albumin and HepPar1), and features of bile ducts (CK19 [cytokeratin 19]) to identify lineages of stem cells differentiating toward the hepatocytic or bile ductular compartments of end-stage cirrhotic human liver. PMID: 27879410
    19. Foxa2, a novel protein partner of the tumour suppressor menin, is deregulated in mouse and human MEN1 glucagonomas. PMID: 28188614
    20. The pattern of FOXA2 mutations and expression in tumors suggests complex regulation and a haploinsufficient or dominant-negative tumor suppressor function. PMID: 27538367
    21. Study demonstrated downregulation of expression of pancreatic master genes SOX9, FOXA2, and GATA4 (2-, 5-, and 4-fold, respectively) and in PANC1 pancreatic cancer cell line stimulated with TGFbeta1 PMID: 27599506
    22. The definitive endoderm and foregut endoderm differentiation capabilities of Wnt pathway-modulated cells were determined based on the expression levels of the endodermal transcription factors SOX17 and FOXA2 and those of the transcription activator GATA4 and the alpha-fetoprotein (AFP) gene, respectively. PMID: 26861571
    23. Our results suggest that FOXA2 promotes cell proliferation, maintains cancer stem cells, favors the development of Triple-Negative/Basal-like tumors, and is associated with increase relapses. PMID: 26298189
    24. FOXA2 regulates a network of genes involved in critical functions of human intestinal epithelial cells. PMID: 25921584
    25. we propose that haploinsufficiency for FOXA2 combined with a decreased expression of NODAL is the likely cause for syndromic BA in this proband. PMID: 25765999
    26. DEANR1 facilitates FOXA2 activation by facilitating SMAD2/3 recruitment to the FOXA2 promoter PMID: 25843708
    27. Expression of FOXA2 is reduced in gastric adenocarcinoma tissues and its low-expression is correlated with malignant clinical pathological features PMID: 25940296
    28. results provide opportunities to understand the FOXA2-centered transcriptional regulation network and novel therapeutic targets to modulate this network in p53-deficient lung cancer PMID: 26093302
    29. FOXA2 as a core transcriptional modulator for pulmonary xenobiotic metabolic pathways; regulates pulmonary expression of CYP2A13 and ABCB1 PMID: 25667220
    30. Results show that miR-141 functions as a tumor suppressor in hepatocellular carcinoma cells through the inhibition of HNF-3beta translation. PMID: 25425543
    31. Study confirmed that FOXA2 inhibited EMT in breast cancer cells by regulating the transcription of EMT-related genes such as E-cadherin and ZEB2. PMID: 25779673
    32. identification of transcription factors specific to early mesoderm commitment; FOXA2 observed to be the most significantly differentially expressed at human embryonic stem cell-endothelial cell day 2; FOXA2 transcription start site is marked with histone modifications for gene activation and repression PMID: 23618383
    33. Results suggest that FOXA2 induced the differentiation of gastric cancer and highlight FOXA2 as a novel therapeutic tartet and prognostic marker for human gastric cancer. PMID: 25129104
    34. we proved that FOXA2 repressed the transcription of matrix metalloproteinase (MMP)-9 and exerted its antimetastasis effect partially through downregulation of MMP-9. PMID: 25142974
    35. this study demonstrates that SIRT1 controls the acetylation level of FOXA2 in a nutrient-dependent manner and in times of nutrient shortage the interaction between SIRT1 and FOXA2 is reduced. PMID: 24875183
    36. Collectively, these studies demonstrated that Mycoplasma pneumoniae induces airway mucus hypersecretion by modulating the STAT/EGFR-FOXA2 signaling pathways. PMID: 25287927
    37. results suggest a novel and unexpected role for DNA methylation in the activation of FoxA2 gene expression during differentiation PMID: 25016019
    38. FOXA2 is acting as a master regulator of key pathways that are enriched for loci implicated by GWAS for most trait categories, with the clear exception of inflammation, suggesting that this factor exerts its effect via noninflammatory processes. PMID: 24878043
    39. Respiratory epithelial cells express FOXA2 during different stages of lung development. PMID: 24970044
    40. Knockdown of FoxO1 and ectopic expression of FoxA2 significantly decreased hepatitis C virus replication. PMID: 24478438
    41. our study indicates that beta-catenin regulates Foxa2 expression, and this interaction is possibly essential to control cell cycle progression during endometrial hyperplasia formation PMID: 22945641
    42. Upregulation of Foxa2 expression can enhance cIAP1, which inhibits the hepatocyte apoptosis. PMID: 23275033
    43. epigenetic silencing of Foxa2 is associated with an increased DNA methylation level and histone H4 (Lys-20) trimethylation, decreased histone H3 acetylation and histone H3 (Lys-4) trimethylation, involved in cellular replicative or premature senescence. PMID: 23391216
    44. VEGF may first downregulate expression of Foxa2 by enhancing Nur77 activity PMID: 22877565
    45. These findings suggest that Foxa2 negatively regulates the basal transcription and expression of the human FTO gene. PMID: 23236435
    46. Human Foxa2 efficiently promoted the incorporation of mesenchymal stem cells into damaged liver tissues. PMID: 22432472
    47. The main mechanism of downregulation of Foxa2 is epigenetic silencing through promoter hypermethylation PMID: 22341411
    48. FOXA2 rs1209523 was not significantly associated with fasting plasma glucose in glucose-tolerant individuals from the general Danish population of middle-aged people PMID: 22325233
    49. These results suggest that increased sensitivity of the polymorphic matrix metalloproteinase 7 promoter to FOXA2 provides one of the genetic bases for the upregulation of matrix metalloproteinase 7 in idiopathic pulmonary fibrosis. PMID: 22268124
    50. pFOXA2 expression is positively correlated with IKKalpha and activated NOTCH1 expression in tumor tissues. PMID: 22196886

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
  • 数据库链接：

    HGNC: 5022

    OMIM: 600288

    KEGG: hsa:3170

    STRING: 9606.ENSP00000315955

    UniGene: Hs.155651