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  4. Recombinant Human Histone H2B type 2-E (HIST2H2BE)

Recombinant Human Histone H2B type 2-E (HIST2H2BE)

  • 货号：
    CSB-YP620981HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP620981HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP620981HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP620981HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP620981HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HIST2H2BE
  • Uniprot No.：
  • 别名：
    HIST2H2BE; H2BFQHistone H2B type 2-E; Histone H2B-GL105; Histone H2B.q; H2B/q
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-126
  • 氨基酸序列
    PEPAKSAPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSI YVYKVLKQVH PDTGISSKAM GIMNSFVNDI FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • 基因功能参考文献：
    1. Findings uncover a novel function of let-7 miRNAs as regulators of H2B ubiquitylation, suggesting an additional mechanism whereby these miRNAs can exert their tumor-suppressive effects. PMID: 28604753
    2. Within the nucleus, PKC-theta; catalytic activity maintains the Ser536 phosphorylation on the p65 subunit of NF-kappaB (also known as RelA) and can directly influence chromatin accessibility at transcriptional memory genes by regulating H2B deposition. PMID: 27149922
    3. Study provides the first observation of the proteolytic processing of histones H2B and H3 in human hepatocytes where four different clipping sites were localized in H3 and one in H2B. PMID: 26424599
    4. Gene expression analysis identified deregulation of histone H2A and H2B genes in all four cell lines ;histone pathways are associated with epirubicin resistance PMID: 26852132
    5. The 2.7-A-resolution crystal structure of the human YL1-H2A.Z-H2B complex shows that YL1 binding, similarly to ANP32E binding, triggers an extension of the H2A.Z alphaC helix. PMID: 26974126
    6. CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent DNA repair resection. PMID: 27098497
    7. analysis of a functional hotspot on ubiquitin that is required for the stimulation of human Dot1 PMID: 26240340
    8. Results show that the cotranscriptional ubiquitylation of histone H2B suggests that it may be more intimately involved in ongoing nucleosome turnover in the wake of Pol II. PMID: 25049226
    9. H2B K34 ubiquitylation by the MOF-MSL complex is part of the protein networks involved in early steps of transcription elongation. PMID: 24837678
    10. Knockdown of EZH2 increased the ubiquitination level of H2BK120. PMID: 24339737
    11. Autoimmunity to isoAsp histone H2B suggests that this form of the autoantigen may be critical in the induction of anti-histone autoantibodies in human SLE. PMID: 22967069
    12. after H2B monoubiquitylation H2B is largely positioned at the exon-intron boundaries of highly expressed exons, and it demonstrates increased occupancy in skipped exons compared with flanking exons in the human genome PMID: 22421545
    13. cancerous cells in the tumor specimens. Taken together, our data suggest that glucose deficiency and loss of uH2B are novel properties of cancer cells in vivo, which may represent important regulatory mechanisms of tumorigenesis PMID: 22615809
    14. H2B and H4 histones were mobilized during herpes simplex virus 1 infection and became available to bind to viral genomes. PMID: 21994445
    15. Studies indicate that H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40. PMID: 21827756
    16. these data point at acetylation of Lysine 120 of H2B as an early mark of poised or active state and establish a temporal sequence between acetylation and mono-ubiquitination of this H2B residue. PMID: 21739721
    17. Protein phosphatase 2C gamma binds to/dephosporylates histone 2b to affect chromatin function. PMID: 17074886
    18. A redox-modulated direct p38/GAPDH-Oct-1 interaction nucleates the occupancy of the H2B promoter by the OCA-S complex, in which p36/LDH plays a critical role in the hierarchical organization of the complex. PMID: 18682386
    19. These studies establish the natural H2B ubiquitylation factors in human cells and also detail the mechanistic basis for H2B ubiquitylation and function during transcription PMID: 19410543
    20. NO-mediated H2B gene repression depends on modifications of endogenous PPAR ligands. PMID: 19425504

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 数据库链接：

    HGNC: 4760

    OMIM: 601831

    KEGG: hsa:8349

    STRING: 9606.ENSP00000358151

    UniGene: Hs.2178