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  4. Recombinant Human Nuclear receptor subfamily 4 group A member 2 (NR4A2)

Recombinant Human Nuclear receptor subfamily 4 group A member 2 (NR4A2)

In Stock
  • 货号：
    CSB-YP016063HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NR4A2
  • Uniprot No.：
  • 别名：
    HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; Nr4a2; NR4A2_HUMAN; nuclear receptor of T cells; nuclear receptor related 1; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; nur related protein-1; human homolog of; Nurr 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; RNR 1; RNR1; T cell nuclear receptor NOT; T-cell nuclear receptor NOT; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related ; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    Yeast
  • 分子量：
    68.6 kDa
  • 表达区域：
    1-598aa
  • 氨基酸序列
    MPCVQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITATTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQHSHLPPQSEEMMPHSGSVYYKPSSPPTPTTPGFQVQHSPMWDDPGSLHNFHQNYVATTHMIEQRKTPVSRLSLFSFKQSPPGTPVSSCQMRFDGPLHVPMNPEPAGSHHVVDGQTFAVPNPIRKPASMGFPGLQIGHASQLLDTQVPSPPSRGSPSNEGLCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCLANKNCPVDKRRRNRCQYCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKSPQEPSPPSPPVSLISALVRAHVDSNPAMTSLDYSRFQANPDYQMSGDDTQHIQQFYDLLTGSMEIIRGWAEKIPGFADLPKADQDLLFESAFLELFVLRLAYRSNPVEGKLIFCNGVVLHRLQCVRGFGEWIDSIVEFSSNLQNMNIDISAFSCIAALAMVTERHGLKEPKRVEELQNKIVNCLKDHVTFNNGGLNRPNYLSKLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPAIIDKLFLDTLPF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The gene fragment corresponding to the 1-598aa of the human NR4A2 protein was synthesized, with appropriate restriction sites suitable for in-frame cloning into an expression vector, with N-terminal 6xHis tag. The yeast was transfected with the expression vector, and the clone was expressed upon certain induction. After the induced cell centrifugation, the recombinant protein was purified from the cell extract and presented as N-terminal 6xHis-tagged fusion. This recombinant human NR4A2 protein's purity is greater than 90% assayed by SDS-PAGE. The NR4A2 protein ran to a band of about 55 kDa molecular weight on the gel.

    Nuclear Receptor 4A2 (NR4A2/NURR1) belongs to the member of the steroid-thyroid hormone-retinoid receptor superfamily. NR4A2 mutations can cause intellectual disability and language impairment with persistent Dystonia-Parkinsonism. In addition, NR4A2 regulates autophagy and chemoresistance in pancreatic ductal adenocarcinoma. Also, misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. De novo variants of NR4A2 are associated with neurodevelopmental disorder and epilepsy. Genome-Wide analysis identifies NURR1-Controlled network of new synapse formation and cell cycle arrest in human neural stem cells.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
  • 基因功能参考文献：
    1. PARP1 inhibitor also suppressed the aldosterone secretion in response to the angiotensin II. Together, these results suggest PARP1 is a prime coregulator for Nurr1. PMID: 29738496
    2. Data expand the understanding of the mechanism by which the NR4A2 nuclear receptor can facilitate DNA DSB repair. PMID: 28607006
    3. An association was identified in a Mexican population between genotype and mRNA expression levels of NR4A2 in patients with Parkinson's disease. PMID: 28716280
    4. miR-34 was identified as a direct negative regulator of NR4A2. A novel regulatory network linking p53, miR-34, and NR4A2 was revealed, in which p53 can overcome its inhibition by endogenous NR4A2 through upregulating miR-34. PMID: 27121375
    5. Nurr1 was induced during intestinal regeneration after I/R injury. Nurr1 promoted proliferation of intestinal epithelial cells after H/R injury. Nurr1 inhibited p21 expression in a p53-independent manner. Nurr1 inhibited p21 gene transcription by binding to p21 promoter directly. PMID: 27553040
    6. Our findings of a de novo deletion of NR4A2 in an individual with mild intellectual disability and prominent speech and language impairment provides further evidence for NR4A2 haploinsufficiency being causative for neurodevelopmental and particularly language phenotypes PMID: 28544326
    7. NR4A sub-family of nuclear orphan receptors (Nor-1, Nurr-1 and Nur-77) may have a role in trophoblastic cell differentiation. PMID: 28808448
    8. this study shows over-expression of NR4A2 mRNA in peripheral CD4+ T cells of Sjogren's syndrome patients PMID: 28621822
    9. NR4A2 and NR4A3 are components of a downstream transcriptional response to PKA activation in the neutrophil, and that they positively regulate neutrophil survival and homeostasis. PMID: 28637666
    10. Study found a marked down-regulated gene expression of the NR4A subfamily (NR4A1, NR4A2, and NR4A3) obtained from Parkinson's disease patients, but only a NR4A1 decrease in Alzheimer's disease patients compared to healthy controls. This study reports that the entire NR4A subfamily and not only NR4A2 could be systemically involved in Parkinson's disease. PMID: 27159982
    11. show that unsaturated fatty acids also interact with the Nurr1 LBD, and solution NMR spectroscopy reveals the binding epitope of DHA at its putative ligand-binding pocket PMID: 27128111
    12. NURR1 is an essential transcription factor for the differentiation, maturation, and maintenance of midbrain dopaminergic neurons. PMID: 26678495
    13. Nurr1 overexpression exerts neuroprotective and anti-inflammatory roles via down-regulating CCL2 in both in vivo and in vitro Parkinson's disease models, contributing to developing mechanism-based and neuroprotective strategies against PD PMID: 27940361
    14. In the PPI network, genes may be involved in Down syndrome (DS) by interacting with others, including nuclear receptor subfamily 4 group A member 2 (NR4A2)early growth response (EGR)2 and NR4A2EGR3. Therefore, RUNX1, NR4A2, EGR2, EGR3 and ID4 may be key genes associated with the pathogenesis of DS. PMID: 27667480
    15. Our results suggest that NLK inhibits transcriptional activation of Nurr1 gene by impeding CBP's role as a co-activator of NF-kappaB and CREB in prostate cancer. PMID: 27036119
    16. decreased expression levels of Nurr1 were associated with chronic inflammation and insulin resistance in patients with T2D. PMID: 26239742
    17. The immunohistochemical, qRT-PCR and western blot analyses revealed that Nurr1 expression was increased in gastric cancer tissues compared with normal gastric tissue. PMID: 26022133
    18. NR4A2 is a key factor in multiple diseases, such as inflammation, cancer and cardiovascular diseases. PMID: 25917081
    19. A2M is expressed in the vasculature and NR4A receptors modulate VSMC MMP2/9 activity by several mechanisms including the up-regulation of A2M. PMID: 25809189
    20. In this review, a concise overview of the current understanding of the important metabolic roles governed by NR4A members NR4A1, NR4A2 and NR4A3 including their participation in a number of diseases shall be provided PMID: 25089663
    21. Data show that some rexinoids display selective coactivator (CoA) recruitment by the retinoid X receptors (RXRs) homodimer and by the heterodimers nuclear receptor Nur77/RXR and Nurr1/RXR. PMID: 26148973
    22. Thus our data identify a previously unknown role for Nr4a2 in the regulation of macrophage polarization. PMID: 25953901
    23. The results failed to reveal significant link between NR4A2 polymorphism and schizophrenia risk. [META-ANALYSIS] PMID: 25982322
    24. Because the phosphorylation site mutants of NR4A2 cannot rescue the cell death-promoting activity, ASK1-p38 pathway-dependent phosphorylation and subsequent cytoplasmic translocation of NR4A2 may be required for oxidative stress-induced cell death. PMID: 25752609
    25. gestational diabetes mellitus, but not pre-existing maternal obesity, associated with increased placental expression PMID: 25199433
    26. NURR1 upregulation by tissue-type plasminogen activator during ischemic stroke is associated with endothelial dysfunction and inflammation. PMID: 25503547
    27. High cytoplasmic expression of NR4A2 is a potential unfavorable prognostic factor for patients with nasopharyngeal carcinoma. PMID: 23803035
    28. The AngII-NGFIB/NURR1 pathway controls HSD3B1 expression. PMID: 25092869
    29. our data demonstrate that key midbrain dopamine regulators (Nurr1, Pitx3, and Lmx1a) play overlapping as well as distinct roles during neurogenesis and neurotransmitter phenotype determination of mDA neurons PMID: 24172139
    30. The current review describes the role of two such factors, Nurr1 and engrailed, in differentiation, maturation, and in normal physiological functions including acquisition of neurotransmitter identity. PMID: 24685177
    31. Data indicate that expression of Nurr1 was significantly induced by 1,1-bis(3'-indolyl)-1-(p-chlorophenyl methane) (DIM-D). PMID: 23950896
    32. A novel role for the NR4A2 nuclear receptors as direct facilitators of nucleotide excision repair. PMID: 24223135
    33. The results of this study suggest a protective and anti-inflammatory role of NR4A2 and TNFAIP3, both being under-regulated in monocytes and CD4 + T cells of MS patients PMID: 24852325
    34. NR4A2 is regulated by gastrin and influences cellular responses of gastric adenocarcinoma cells. PMID: 24086717
    35. High NR4A2 expression in gastric cancer cells confers chemoresistance, attenuates 5-fluorouracil-induced apoptosis, and predicts an unfavorable survival, especially for those who received chemotherapy. PMID: 23821160
    36. Bioinformatic and deletion studies identified a 5' region of the TSP-1 promoter repressed by NR4A2 and proangiogenic transcription factors, including NF-kappaB and Ets1/2. PMID: 23933487
    37. Nurr1 overexpression significantly increased the SIRT1 occupancy of the consensus elements for Nurr1 binding hTH promoter region. PMID: 23977047
    38. NR4A nuclear receptors are involved in negative selection of thymocytes, Treg differentiation and the development of Ly6C monocytes. Nur77 and Nurr1 attenuate atherosclerosis in mice whereas NOR-1 aggravates vascular lesion formation. PMID: 24005216
    39. High nurr1 is associated with progression of prostate cancer PMID: 23679312
    40. in human melanoma HMV-II cells both CRF and Ucn1 regulate TRP1 gene expression via Nurr-1/Nur77 production, independent of pro-opiomelanocortin or alpha-melanocyte-stimulating hormone stimulation. PMID: 23416839
    41. High expression of NR4A2 in CRC cells confers chemo-resistance, attenuates chemotherapeutics-induced apoptosis, and predicts unfavorable prognosis of colon cancer patients. PMID: 23809767
    42. Recessive genotypes of rs1150143, rs1150144, rs834830, and rs707132 were associated with a worse sustained attention task performance in schizophrenic males, but not females. Males with the GGGTG haplotype had poorer performance. PMID: 22294735
    43. NURR1 function presents a dichotomy in breast cancer etiology, in which NURR1 expression is associated with normal breast epithelial differentiation and efficacy of systemic cancer therapy. PMID: 23517088
    44. PIASgamma fully represses Nurr1 transactivation through a direct interaction, independently of its E3-ligase activity PMID: 23358114
    45. Collectively, our results suggest that NR4A2 may be a susceptibility gene for Parkinson's disease in the Chinese population PMID: 22827504
    46. Nurr1 shuttling between the cytosol and nucleus is controlled by specific nuclear import and export signals PMID: 23283970
    47. Nur-related receptor 1 gene polymorphisms are implicated in alcohol dependence in Mexican Americans PMID: 23066323
    48. Data show altered adipose tissue expression of the NURR1 stress-responsive nuclear receptor in obesity, suggesting it may modulate pathogenic potential in humans. PMID: 22143616
    49. Pin1 enhances the transcriptional activity of all three NR4A nuclear receptors and increases protein stability of Nur77 through inhibition of its ubiquitination. PMID: 22789442
    50. analysis of Nurr1 gene expression in electrically-stimulated human MSCs and the induction of neurogenesis PMID: 22800541

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Mostly nuclear; oxidative stress promotes cytoplasmic localization.
  • 蛋白家族：
    Nuclear hormone receptor family, NR4 subfamily
  • 组织特异性：
    Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
  • 数据库链接：

    HGNC: 7981

    OMIM: 601828

    KEGG: hsa:4929

    STRING: 9606.ENSP00000344479

    UniGene: Hs.563344