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  4. Recombinant Human Pituitary homeobox 2 (PITX2)

Recombinant Human Pituitary homeobox 2 (PITX2)

  • 货号：
    CSB-YP860349HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP860349HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP860349HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP860349HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP860349HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    All1 responsive gene 1; ALL1 responsive protein ARP1; ALL1-responsive protein ARP1; ARP 1; ARP1; Brx 1; Brx1; Homeobox protein PITX2; IDG 2; IDG2; IGDS 2; IGDS; IGDS2; IHG 2; IHG2; IRID 2; IRID2; MGC111022; MGC20144; Otlx 2; Otlx2; Paired like homeodomain transcription factor 2; Paired-like homeodomain transcription factor 2; Pituitary homeo box 2; Pituitary homeobox 2; PITX 2; pitx2; PITX2_HUMAN; PTX 2; PTX2; RGS; RIEG 1; RIEG; Rieg bicoid related homeobox transcription factor 1; RIEG bicoid related homeobox transcription factor; RIEG bicoid-related homeobox transcription factor; RIEG1; RS; Solurshin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-317
  • 氨基酸序列
    METNCRKLVS ACVQLGVQPA AVECLFSKDS EIKKVEFTDS PESRKEAASS KFFPRQHPGA NEKDKSQQGK NEDVGAEDPS KKKRQRRQRT HFTSQQLQEL EATFQRNRYP DMSTREEIAV WTNLTEARVR VWFKNRRAKW RKRERNQQAE LCKNGFGPQF NGLMQPYDDM YPGYSYNNWA AKGLTSASLS TKSFPFFNSM NVNPLSSQSM FSPPNSISSM SMSSSMVPSA VTGVPGSSLN SLNNLNNLSS PSLNSAVPTP ACPYAPPTPP YVYRDTCNSS LASLRLKAKQ HSSFGYASVQ NPASNLSACQ YAVDRPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
  • 基因功能参考文献：
    1. Minor allele of SNP rs2200733 is associated with the risk of preeclampsia. SNP rs220073 may represent a common risk factor that predispose women to develop both preeclampsia during pregnancy and cardiovascular disease later on. PMID: 30177054
    2. This study for the first time demonstrates that the PITX2 mutation could lead to non-syndromic orodental anomalies in humans. We propose that the specific location in the C-terminal domain of PITX2 is exclusively necessary for tooth development. PMID: 29121437
    3. Rs17042171, near PITX2 on chromosome 4q25, is associated with atrial fibrillation susceptibility in the Chinese Han population from the central plains, suggesting that this SNP can provide a new strategy for clinical diagnosis in atrial fibrillation patients. PMID: 30110000
    4. NMR methodology was employed for determining the dynamics of lysine side-chain amino groups via (15)N relaxation measurements in the Lys50-class homeodomains from the Drosophila protein Bicoid and the human protein Pitx2 PMID: 29664630
    5. Results demonstrate that BBP decreases endometrial mesenchymal stem/stromal cell (EN-MSC) myogenic differentiation through up-regulation of miR-137 and decreased transcription of PITX2. Also, BBP affects PITX2 expression through miR-137 targeting the 3' untranslated region of PITX2 mRNA. PMID: 28298639
    6. For non-metastatic triple-negative breast cancer patients, selective determination of the PITX2 DNA-methylation status may serve as a cancer biomarker for predicting response to anthracycline-based adjuvant chemotherapy. PMID: 29328369
    7. MiR-21 was down-regulated while PITX2 was up-regulated in pituitary adenoma tissues; MiR-21 can inhibit pituitary adenoma cell HP75 proliferation and facilitate apoptosis via inhibiting PITX2 expression PMID: 28742208
    8. PITX2 and PANCR methylation status were shown to be independent predictors for overall survival in HNSCC patients. Tissue-based methylation testing could therefore potentially be employed to identify patients with a high risk for death who might benefit from a more radical or alternative treatment. PMID: 27716615
    9. Data suggest that mutations affecting conserved non-coding elements of PITX2 may constitute an important class of mutations in patients with ASD for whom the molecular cause of their disease have not yet been identified. PMID: 28911203
    10. we propose that the Smad4-Pitx2-PPP2R2A axis, a new signaling pathway, suppresses the pancreatic carcinogenesis PMID: 26848620
    11. Pitx2 is essential to maintain iHepSCs stem cell characteristics PMID: 27697592
    12. Glaucoma prevalence and phenotype are characterized in a cohort of glaucoma patients and their family members with PITX2 variants. PMID: 28513611
    13. Using an independent analytical platform, PITX2 methylation was validated as a prognostic biomarker in HNSCC patients, identifying patients that potentially benefit from intensified surveillance and/or administration of adjuvant/neodjuvant treatment, i.e. immunotherapy. PMID: 28617833
    14. Pitx2 promoter region is methylated in atrial fibrillation patients. PMID: 28427903
    15. Findings suggest ESR1 and PITX2 promoter methylation may be correlated with a worse survival of patients with breast cancer. The clinical utility of aberrantly methylated ESR1 and PITX2 could be a promising factor for the prognosis of breast cancer. PMID: 28700487
    16. PITX2 methylation was significantly increased in tumor-positive biopsies and strongly correlated with International Society of Urological Pathology (ISUP) grade groups. PMID: 27939865
    17. the atrial fibrillation (AF)-associated SNP rs2595104 altered PITX2c expression via interaction with TFAP2a; such a pathway could ultimately contribute to AF susceptibility at the PITX2 locus associated with AF PMID: 27866707
    18. Study showed that PITX3 and PITX2 were hypermethylated in prostate carcinomas (PCa) and significantly associated with established clinicopathologic parameters characteristic of PCa. PMID: 27708722
    19. The PITX2 gene expression level in prostate cancer tissues was lower than that in benign tissues. A higher degree of PITX2 DNA methylation was associated with higher tumor stage and lower survival rates. PITX2 DNA methylation presents a good predictive value for prostate cancer survival. PMID: 27173224
    20. This is the first study reporting on bi-allelic changes of PITX2 potentially contributing to a more severe Axenfeld-Rieger syndrome (ARS) phenotype. PMID: 27009473
    21. The role of PITX2 in glaucoma may be mediated partly by regulating the expression of CXCL6 and BBS5 and thus affecting immune functions and intraocular pressure. PMID: 27520585
    22. Chronic Atrial fibrillation increases Pitx2c expression in isolated human atrial myocytes PMID: 26714926
    23. ARS is an autosomal dominant disorder with high penetration. It is primarily caused by a mutation of the pituitary homeobox 2 (PITX2) or forkhead box C1 (FOXC1) gene. Mutations in PITX2 on chromosome 4q25, or in FOXC1 at 6p25, have been identified in approximately 40% to 70% of patients with ARS PMID: 26240509
    24. Expression of PITX2 in BM of early-stage breast cancer patients is associated with risk for early disease recurrence. PMID: 26400846
    25. A novel loss-of-function PITX2 mutation (Q102L) co-segregated with tetralogy of Fallot with complete penetrance. PMID: 26657035
    26. Our study uncovers the PITX2-induced expression of TGFB1/2/3 as well as INHBA genes (p < 0.01) followed by SMAD2/3-dependent TGF-b signalling pathway in ovarian cancer cells PMID: 26298390
    27. Both ZFHX3 and PITX2c regulate expression of NPPA, TBX5 and NKX2.5. PMID: 26267381
    28. PANCR knockdown decreased PITX2c expression in differentiated cardiomyocytes, altering the transcriptome in a manner similar to PITX2c knockdown. PMID: 26783232
    29. PITX2 loss-of-function mutation has a role in increased susceptibility to Congenital Endocardial Cushion Defect and Axenfeld-Rieger Syndrome PMID: 25893250
    30. The results suggest an association between PITX2-related SNPs and dementia. PMID: 25494715
    31. The Axial level-specific regulation of neuronal development of brain requirements for Pitx2 during neuronal migration and differentiation. PMID: 25124216
    32. A mutation in the 5' untranslated region of the PITX2 gene significantly downregulates PITX2 expression in atrial myocytes in patients with extreme atrial fibrillation. PMID: 25391453
    33. Single nucleotide polymorphism (rs2200733) located in proximity of the gene PITX2 (paired-like homeodomain transcription factor 2) was highly associated with atrial fibrillation. PMID: 25443231
    34. study strengthens prior findings that PITX2 methylation is useful as a biomarker of poor outcome of PCa and in addition we also suggest that it may be particularly useful in men with low Gleason score. PMID: 25402584
    35. High PITX2 expression is associated with invasive ductal carcinoma of the breast. PMID: 25339043
    36. The homeodomain transcription factor PITX2 is required for specifying correct cell fates and establishing angiogenic privilege in the developing cornea. PMID: 25044936
    37. Pitx2-mediated repression of Depdc1b expression contributes to the regulation of multiple molecular pathways, such as Rho GTPase signaling. PMID: 25704760
    38. Genome-wide association analysis linked the FOXC1-interacting transcription factor PITX2 to cerebral small-vessel disease. PMID: 25250569
    39. This is the first study to link PITX2c mutations to familial atrial fibrillation. PMID: 23611745
    40. The combination of Pitx2, a regulator of dental stem cells and miR-200a converts mesenchymal cells to a fully differentiated dental epithelial cell type. PMID: 25122764
    41. The findings of the study associate PITX2c loss-of-function mutations with atrial fibrillation. PMID: 24473555
    42. study demonstrates the association between PITX2c loss-of-function mutations and the transposition of the great arteries and ventricular septal defect in humans, providing further insight into the molecular mechanisms responsible for CHD. PMID: 24604414
    43. describe familial cases of TH in two generations (proband and his father), in addition to other two sporadic cases. We have found polymorphisms in the HOXB3, HOXD3, and a new synonymous variant, and PITX2 genes PMID: 24127533
    44. PITX2c expression in human adult left atrial appendages is not associated with the chromosome 4q25 AF risk SNPs; thus, the mechanism by which these SNPs are associated with AF remains enigmatic. PMID: 24465984
    45. overexpression of PITX2, a paired-like homeodomain transcription factor and a downstream effector of Wnt/beta-catenin signaling, resulted in upregulation of cyclin A1 in HEK293 cells and TPC-1 thyroid cancer cells. PMID: 24002705
    46. We detected a novel frameshift mutation p.M66Ifs*133 in PITX2 in a Chinese family with ARS. PMID: 24390743
    47. Association of a novel PITX2c loss-of-function mutation with familial atrial fibrillation. PMID: 24333117
    48. Pitx2 positively regulates miR-17-92 and miR-106b-25. PMID: 24927531
    49. PITX2 gene was heterozygous for a 2-bp deletion and an insertion of T, a frameshift mutation predicted, to result in premature termination at the 54th amino acid of the PITX2 protein. PMID: 24003428
    50. PITX2 forms complex with histone H3 lysine 4 (H3K4) methyltransferase. PITX2 complex methylates H3K4. PMID: 24486544

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  • 相关疾病：
    Axenfeld-Rieger syndrome 1 (RIEG1); Anterior segment dysgenesis 4 (ASGD4); Ring dermoid of cornea (RDC)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family, Bicoid subfamily
  • 数据库链接：

    HGNC: 9005

    OMIM: 137600

    KEGG: hsa:5308

    STRING: 9606.ENSP00000304169

    UniGene: Hs.643588