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  4. Recombinant Human Type-1 angiotensin II receptor (AGTR1), partial

Recombinant Human Type-1 angiotensin II receptor (AGTR1), partial

  • 货号：
    CSB-YP001465HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    AG2S; Agtr 1; Agtr1; AGTR1_HUMAN; Agtr1a; AGTR1B; Ang II; Angiotensin II receptor type 1; Angiotensin II type-1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT 1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; Type 1B angiotensin II receptor; Type-1 angiotensin II receptor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    9.2kDa
  • 表达区域：
    297-359aa
  • 氨基酸序列
    LNPLFYGFLGKKFKRYFLQLLKYIPPKAKSHSNLSTKMSTLSYRPSDNVSSSTKKPAPCFEVE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The preparation of Recombinant Human AGTR1 protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This AGTR1 was expressed in Yeast at and fused with N-terminal 6xHis tag. According to SDS-PAGE, the purity turns out to be 90%+.

    AGTR1 encodes the angiotensin II (Ang II) type I receptor that belongs to the family of G-protein coupled receptors. AGTR1 is an important part of the Renin-angiotensin system (RAS). AGTR1 has a strong influence on tumor growth, angiogenesis, inflammation, and immunity. Hypomethylation in the AGTR1 promoter had been validated to be inversely correlated with uric acid levels, which can be a significant risk predictor of essential hypertension (EH). Besides, AGTR1 methylation had been extensively studied in human cancers, such as oral squamous cell carcinoma. For example, a study showed that AGTR1 mediates breast cancer metastasis by regulating CXCR4/SDF-1α. Another study showed that AGTR1 promotes the invasion of breast cancer. Based on the pan-cancer analysis, researchers found that the expression of AGTR1 in most tumor tissues, including lung cancer, was significantly lower than the corresponding normal tissues and was related to prognosis.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.; (Microbial infection) During SARS coronavirus-2/SARS-CoV-2 infection, it is able to recognize and internalize the complex formed by secreted ACE2 and SARS-CoV-2 spike protein through DNM2/dynamin 2-dependent endocytosis.
  • 基因功能参考文献：
    1. Intracrine action of angiotensin II in mesangial cells: subcellular distribution of angiotensin II receptor subtypes AT1 and AT2. PMID: 29455433
    2. Meta-analysis suggests that AGTR1 A1166C polymorphism may increase the susceptibility to diabetic nephropathy. PMID: 30313019
    3. Results may indicate that the choice of diet can undermine the potential genetic risk of the AT1R 1166A>C polymorphism for MetS, and persons who are variant carriers may spontaneously choose the Mediterranean diet. PMID: 30205174
    4. The research reveals that among patients with essential hypertension treated with hypotensive drugs there are certain relationships between the rs5182 and rs5186 polymorphisms of the AGTR1 gene, as well as between the rs1799998 polymorphism of the CYP11B2 gene and the volume of the carotid bodies. PMID: 29627490
    5. The protective effects of short-term hypoxia (STH) are associated with the upregulation of MasR to inhibit AT1 R signaling. STH could be a potential therapeutic strategy for cardiac diseases in hypertensive patients. PMID: 29052864
    6. A high level of ART1 antibodies was a risk factor for allograft function in kidney transplant recipients. PMID: 29507275
    7. we found an interaction between ACE2 and AGTR1 in structuralatrial fibrillation patients in a Chinese Han population PMID: 29441892
    8. AGTR1 DNA hypomethylation is a risk factor for the development of hypertension. Furthermore, there is a correlation between smoking, diet, and AGTR1 DNA methylation levels. PMID: 28376480
    9. Chinese Han persons with C allele of rs9817428 in PPARG gene were related to the increased risk of hypertension. PMID: 29266977
    10. Association of AGTR1 single nucleotide polymorphism rs275645 and pre-eclampsia in Chinese population. PMID: 27910864
    11. although the present study did not find any association between AGTR1 A1166C polymorphism and the risk of systemic lupus erythematosus, the presence of this polymorphism was associated with higher levels of malondialdehyde and higher concentration of neopterin PMID: 28530466
    12. We conclude that the crosstalk between angiotensin AT1 receptor and insulin receptor signaling shows a high degree of specificity, and involves Galphaq protein, and activation of distinct kinases. Thus, the BRET(2) technique can be used as a platform for studying molecular mechanisms of crosstalk between insulin receptor and 7TM receptors. PMID: 28854843
    13. The AGTR1-mediated cell apoptosis was suppressed by overexpressing miR-1248 and inhibited protein translation of AGTR1 by miR-1248, through binding to the 3'-UTR of the AGTR1 mRNA. PMID: 29482191
    14. The effects ofgenetic polymorphisms and diet on the risk of metabolic disorders predisposing to cardiovascular diseases among Malay, Chinese, and South Asian persons are reported. PMID: 28792482
    15. AGTR1 gene, related to renin-angiotensin system, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis. PMID: 29642183
    16. The genotypes of REN, AT1R and AT2R were not associated with the development of preeclampsia in South African Black women. PMID: 28641212
    17. AC-CC genotype of AT1R and noise were the risky factors of EH. These factors also interacted with each other. PMID: 28157766
    18. the A1166C polymorphism in the AGTR1 gene may not be a risk factor for breast cancer (Meta-Analysis) PMID: 26634463
    19. High levels of AT1R is associated with angiogenesis in bladder cancer. PMID: 28599664
    20. High angiotensin II type 1 receptor antibodies are associated with graft rejection in renal transplantation. PMID: 26546592
    21. No statistically significant data on the correlation between the AGTR1 A/C polymorphism and the development of Atrial Fibrillation were obtained in any patient subgroup of a Siberian population. PMID: 29039830
    22. Letter/Case Report: refractory vascular rejection in a hand transplant recipient in the presence of AGTR1 antibodies. PMID: 28767535
    23. These findings suggest that AT1R overexpression is an independent adverse prognosticator for patients with esophageal squamous cell carcinoma PMID: 27564102
    24. As previously found for rs1799752 in ACE, rs5186 in AGTR1 was associated with dementia at baseline. These associations became substantially weaker, or disappeared, when dementia onset to 2012 was included. PMID: 27639288
    25. Study identified the first significant associations between SNPs rs275651, rs275652 and rs4524238 in AGTR1 gene and high-altitude pulmonary edema susceptibility. PMID: 27732943
    26. There was no association for rs5186 with LVH under any of models. PMID: 28513230
    27. Homocysteine displaces angiotensin II and regulates the conformation of the AT1 receptor. PMID: 29296021
    28. no statistical association with kidney graft failure was found for anti-AT1R antibodies, indicating that these tests may not be the best predictors of graft outcome in living donor renal transplantation. PMID: 28372986
    29. Inhibition of CaV1.2 upregulates AT1R signaling in response to angiotensin II. PMID: 28514967
    30. Single nucleotide polymorphisms of AGTR1 gene are associated with Hypertension. PMID: 27045027
    31. The presence of AGTR1 autoantibodies is an independent predictor for aortic stiffness progression in normotensive subjects. PMID: 29097625
    32. The DEGs, such as AGTR1, CYP3A4 and CYP4A11 may play critical roles in the development of HTN likely via the regulation by hsa-miR-26b-5p and taking part in some pathways. PMID: 27756246
    33. Low shear stress induces the generation of endothelial reactive oxygen species via AT1R/eNOS/NO signaling pathway. PMID: 28518223
    34. Low AGTR1 expression is associated with chemoresistance of osteosarcoma. PMID: 28073349
    35. No relationship was found between the studied polymorphisms (14094 ACE gene, rs1800469 gene TGFbeta1, GNB3 gene rs5443, rs5186 AGTR1 gene) and the occurrence of primary vesicoureteral reflux. PMID: 27988909
    36. Angiotensin II type 1 receptors promote ADAM17-mediated ACE2 shedding in the brain of hypertensive patients. PMID: 28512108
    37. Data suggest that allosteric communication between heterodimeric AT1R and PTGFR is mediated through GNAQ and may also involve proximal phospholipase C but not distal protein kinase C signaling partners; PTGFR activation has negligible effects on AT1R-based conformational biosensors. (AT1R = angiotensin II receptor, type 1; PTGFR = prostaglandin F2alpha receptor; GNAQ = GTP-binding protein G[q] subunit alpha) PMID: 28584054
    38. our results show the pivotal mechanisms of AT1R-induced harmful phenotype of Plasmodium-specific CD8(+) T cells during blood-stage malaria. PMID: 28261571
    39. Ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension. PMID: 27496272
    40. Mutations within transmembrane domains IV, V, VI, and VII had no effect on angiotensin-mediated beta-arrestin1 recruitment; however, they exhibited differential effects on the assembly of AT1R into oligomeric complexes. PMID: 28096461
    41. Our findings provide evidence that induction of APP shedding via Ang II/AT1 receptor stimulation is effected by G protein activation with Gbg subunits playing important roles. PMID: 28102934
    42. highly and selectively expressed in colonic L cells PMID: 27447725
    43. AT1R-Ab and anti-endothelial cell antibodies may identify patients at higher risk for antibody mediated injury, particularly in the presence of HLA-donor specific antibodies. PMID: 27222934
    44. Structure and Function of Cross-class Complexes of G Protein-coupled Secretin and Angiotensin 1a Receptors. PMID: 27330080
    45. studied the clinical significance of the AT1RA1166C receptor polymorphism and its relationship with serum concentration of lipids, oxidant/antioxidant systems and vascular adhesion protein-1(VAP-1) in 100 psoriatic patients and 100 gender- and age-matched unrelated healthy controls from west population of Iran PMID: 26395033
    46. angiotensin II Type 1 receptor genotype may influence the clinical expression of vasculopathy in systemic sclerosis patients. PMID: 27488275
    47. Single nucleotide polymorphisms (SNP) in angiotensin II receptor, type 1 (AGTR1), transcription factor AP-2 beta (TFAP2B), and tumor necrosis factor receptor-associated factor 1 (TRAF1) have been reported to be associated with the incidence of PDA in preterm infants. PMID: 26615960
    48. AGTR1 rs3772622 gene polymorphism was not associated with the risk of developing non-alcoholic fatty liver disease (NAFLD), but could increase the risk of NAFLD patients suffering from coronary artery disease in the Chinese Han population. PMID: 27342049
    49. Results demonstrated that rs1492099, a SNP of AGTR1 gene, could be a potential genetic risk factor of arterio-venous fistula malfunction in male hemodialysis patients. PMID: 27240348
    50. Median total Angiotensin II type 1 receptor IgG concentration was significantly higher in the stable pediatric kidney transplant group as compared to the adult transplant group. For both groups, the transplant group showed higher levels than the control group. PMID: 27251358

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  • 相关疾病：
    Renal tubular dysgenesis (RTD)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Liver, lung, adrenal and adrenocortical adenomas.
  • 数据库链接：

    HGNC: 336

    OMIM: 106165

    KEGG: hsa:185

    STRING: 9606.ENSP00000273430

    UniGene: Hs.477887