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  4. Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-2 (Atf2)

Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-2 (Atf2)

  • 货号：
    CSB-YP002270MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002270MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002270MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002270MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002270MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Atf2; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; EC 2.3.1.48; Activating transcription factor 2; MXBP protein; cAMP response element-binding protein CRE-BP1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-487
  • 氨基酸序列
    MSDDKPFLCT APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RNDSVIVADQ TPTPTRFLKN CEEVGLFNEL ASPFENEFKK ASEDDIKKMP LDLSPLATPI IRSKIEEPSV VETTHQDSPL PHPESTTSDE KEVPLAQTAQ PTSAIVRPAS LQVPNVLLTS SDSSVIIQQA VPSPTSSTVI TQAPSSNRPI VPVPGPFPLL LHLPNGQTMP VAIPASITSS NVHVPAAVPL VRPVTMVPSV PGIPGPSSPQ PVQSEAKMRL KAALTQQHPP VTNGDTVKGH GSGLVRTQSE ESRPQSLQQP ATSTTETPAS PAHTTPQTQN TSGRRRRAAN EDPDEKRRKF LERNRAAASR CRQKRKVWVQ SLEKKAEDLS SLNGQLQSEV TLLRNEVAQL KQLLLAHKDC PVTAMQKKSG YHTADKDDSS EDLSVPSSPH TEAIQHSSVS TSNGVSSTSK AEAVATSVLT QMADQSTEPA LSQIVMAPPS QAQPSGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
  • 基因功能参考文献：
    1. These findings point to an oncogenic function for ATF2 in melanoma development that appears to be independent of its transcriptional activity. PMID: 27210757
    2. adiponectin inhibited endoplasmic reticulum stress and apoptosis of adipocyte in vivo and in vitro by activating the AMPK/PPARalpha/ATF2 pathway. PMID: 27882945
    3. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were used to detect the mRNA and protein expressions of p-p38MAPK, AAT, signal transducer and activator of transcription 1 (STAT1) and activating transcription factor2 (ATF2) PMID: 28358847
    4. increased expression of the gene encoding PKCepsilon and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression PMID: 26645581
    5. These data identified a role for ATF2 in regulating melanocyte responses. PMID: 25963442
    6. suppression of tumorigenesis by JNK requires ATF2. PMID: 25456131
    7. Taken together, our observations indicated that SOCS2 could suppress myotube formation, act as an anti-regulator of mitochondria biogenesis via inhibiting p38 MAPK signal pathway. PMID: 24352701
    8. ATF2 is an important transcriptional factor relating to inflammation through the suppression of ATF3 in M1 macrophages of White adipose tissue. PMID: 23712976
    9. ATF-2 plays critical roles for proper expression of tyrosine hydroxylase gene and for neurite extension of catecholaminergic neurons, possibly through repressor-like action. PMID: 17896792
    10. these studies show that the IL-1beta-induced increase in intestinal tight junction permeability was regulated by p38 kinase activation of ATF-2 and by ATF-2 regulation of MLCK gene activity PMID: 23656735
    11. The ATF2 represses renin expression by drifting the transcriptional control of renin gene away from CRE-binding protein (CREB). PMID: 22628303
    12. ATF2 has a critical role in limiting the activities of stress kinases JNK and p38 which are potent inducers of cell death in the CNS PMID: 21533046
    13. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    14. MITF is downregulated by ATF2 in the skin of Atf2-/- mice, in primary human melanocytes, and in melanoma cell lines. PMID: 21203491
    15. Growth factor-independent signaling pathways converge in the formation of an active c-Jun.AFT2 dimer, which induces the expression of the anti-apoptotic factor Bcl-X(L) that mediates a pro-survival response. PMID: 20507983
    16. Data show that ATF-2 was rapidly phosphorylated and recruited to the Tnfa promoter following ligation of TLR2. PMID: 20018859
    17. ATF-2 plays an important regulatory role in MAG expression at a specific stage of shi/shi oligodendrocyte differentiation. PMID: 12125072
    18. P38 and this protein are involved in osteoblast osmotic response to elevated extracellular glucose PMID: 12149242
    19. Role of ATF2 peptide in apoptosis of melanoma cells PMID: 12208865
    20. a role for ATF2 in the Transcriptional activation of the C/EBPbeta gene PMID: 12215258
    21. ATF4 and ATF2 have roles in regulating CHOP expression PMID: 14630918
    22. JNK was the only MAP kinase that phosphorylated Ser90 by means of ATF-2 in the MAP kinase signaling cascade. PMID: 15304344
    23. syndecan-4 has a role in modulating nuclear ATF-2 transcriptional activity in a Rac1-dependent manner PMID: 15371457
    24. Findings underscore a key regulatory role of ATF-2 in tumor growth and progression of mouse skin tumors. PMID: 15574764
    25. Musk expression was inhibited by CREB interacting with Atf2 and MyoD. PMID: 15964791
    26. activity of the AP-1 components c-Jun, ATF2, and c-Fos is altered in renal cystic tissue of hypomorphic Pkd1 mice with polycystic kidney disease PMID: 16049073
    27. ATF-2 acts as a tumor susceptibility gene of mammary tumors, at least partly by activating a group of target genes, including Maspin and Gadd45alpha. PMID: 17189429
    28. Results show that the absence of ATF-2 in growth plate chondrocytes corresponds to a decline in Bcl-2 promoter activity, as well as a reduction in Bcl-2 protein levels. PMID: 17219413
    29. TNFalpha selectively stimulates the expression of a key component of its own signaling pathway, Map4k4, through a TNFR1-dependent mechanism that targets the transcription factors c-Jun and ATF2 PMID: 17500068
    30. significant increase in active p38 due to loss of a negative feedback loop involving the ATF2-dependent transcriptional activation of MAPK phosphatases in embryonic liver cells PMID: 17699753
    31. Controls the transcription of GADD45alpha and Maspin (SERPIN-B5) independently of tumor suppressor protein p53. PMID: 17700520
    32. These data reveal that loss of ATF2 transcriptional activity serves to promote skin tumor formation, thereby indicating a suppressor activity of ATF2 in skin tumor formation. PMID: 18227516
    33. Results indicated that piperonyl butoxide activated c-Jun and ATF-2 in mouse hepatocytes during the early stage of hepatocarcinogenesis. PMID: 18228000
    34. TGFbeta activates ATF2 phosphorylation and Csrp2 gene expression via a CRE promoter element. PMID: 18387947
    35. ATF-2 regulates expression of pRb within the developing growth plate, contributing to the skeletal phenotype of ATF-2 mutant mice through the regulation of chondrocyte proliferation and differentiation. PMID: 18638549
    36. Data indicate that the ATF3 gene is a bona fide target gene of ATF2 in gonadotrophs. PMID: 18719024
    37. ATF-2 and Sma- and Mad-related protein (SMAD)-3 are, in addition to NF-kappa B, essential for interleukin-23 subunit p19 expression during the response to murine Theiler virus or toll-like receptor (TLR)-3 pathway activation. PMID: 18802055
    38. the phosphorylation of ATF-2 at Ser-121 plays a key role in the c-Jun-mediated activation of transcription that occurs in response to TPA. PMID: 19176525
    39. ATF-2 plays an important role in TLR-mediated transcriptional control in macrophage cells. PMID: 19422799
    40. The sequential nuclear appearance of ERK1/2 and p38 determines the induction of ATF2-Thr71 and ATF2-Thr69+71-phosphorylation in response to serum. PMID: 19647037
    41. Data reveal a new signaling pathway activated by amino acid starvation leading to ATF2 phosphorylation and subsequently positively affecting the transcription of amino acid-regulated genes. PMID: 19822663

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion outer membrane.
  • 蛋白家族：
    BZIP family, ATF subfamily
  • 数据库链接：

    KEGG: mmu:11909

    STRING: 10090.ENSMUSP00000058521

    UniGene: Mm.209903