Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse DNA polymerase lambda (Poll)

Recombinant Mouse DNA polymerase lambda (Poll)

  • 货号：
    CSB-YP874105MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP874105MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP874105MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP874105MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP874105MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Poll; PolkDNA polymerase lambda; Pol Lambda; EC 2.7.7.7; EC 4.2.99.-; DNA polymerase kappa
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-573
  • 氨基酸序列
    MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA GIGRARAELF EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP QLPPGAQLVK STWLSLCLQE GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA EKPSRTQAQL SSEDETSDGE GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA QPSSQKATNY NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW YHQGFRNLED LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS AQAFNPGLLC VACGSYRRGK MTCGDVDVLI THPDGRSHRG IFSCLLDSLR QQGFLTDDLV SQEENGQQQK YLGVCRLPGP GKRHRRLDII VVPYCEFACA LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGPGQVLP TPTEKDVFKH LGLPYREPAE RDW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • 基因功能参考文献：
    1. Results show that deficiency of either DNA polymerases beta or lambda or both results in a modest but significant decrease in V region somatic hypermutation (SHM) with no effect on mutation specificity, suggesting no direct role in SHM. PMID: 24084171
    2. both pol lambda and pol beta interact with the upstream DNA glycosylases for repair of alkylated and oxidized DNA bases PMID: 20805875
    3. analysis of the interaction between DNA Polymerase lambda and anticancer nucleoside analogs PMID: 20348107
    4. Hydrocephalus, situs inversus, chronic sinusitis, and male infertility in DNA polymerase lambda-deficient mice: possible implication for the pathogenesis of immotile cilia syndrome. PMID: 11909969
    5. Pol lambda is not required for normal Ig gene hypermutation. PMID: 11937519
    6. Pol lambda contributes to base excision repair in mouse fibroblast cell extract. PMID: 15749700
    7. Pol lambda protects cells against oxidative stress, and participates in oxidative DNA damage base excision repair. PMID: 16002405

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-X family
  • 数据库链接：

    KEGG: mmu:56626

    STRING: 10090.ENSMUSP00000026239

    UniGene: Mm.46509