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  4. Recombinant Mouse Osteocalcin (Bglap)

Recombinant Mouse Osteocalcin (Bglap)

In Stock
  • 货号：
    CSB-EP002682MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Bglap
  • Uniprot No.：
  • 别名：
    Bglap; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    21.1kDa
  • 表达区域：
    50-95aa
  • 氨基酸序列
    YLGASVPSPDPLEPTREQCELNPACDELSDQYGLKTAYKRIYGITI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    以下是生产重组小鼠骨钙素 (Bglap) 的一般步骤：
    Bglap基因的获得与克隆：从小鼠骨组织中提取总RNA，采用逆转录和PCR扩增技术获得Bglap基因。将放大的 Bglap 基因插入表达载体, 如 pET 系列载体。
    转染宿主细胞：将表达 Bglap 的载体转染到宿主细胞中。可以使用常见的哺乳动物细胞系，如CHO细胞、HEK293细胞等。
    表达Bglap蛋白：根据表达载体中的启动子和调控元件，诱导转染宿主细胞表达Bglap蛋白。培养期间可加入抗生素等药物以维持表达载体的稳定性。
    收集细胞裂解物：通过细胞破碎和离心收集含有 Bglap 蛋白的细胞裂解物。
    纯化Bglap蛋白：采用亲和层析、离子交换层析、凝胶过滤层析等方法纯化Bglap蛋白。
    识别和测定活性：使用 SDS-PAGE、Western blot 等技术来识别和量化纯化的 Bglap 蛋白。此外，可以使用骨钙素活性分析等测定法评估生物活性和功能。
    需要注意的是，骨钙素是一种钙结合蛋白，在表达和纯化过程中对 pH 和温度敏感。因此，在生产过程中必须使用适当的条件。此外，骨钙素在储存和使用过程中可能容易发生蛋白水解降解，因此必须采用适当的储存条件。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 基因功能参考文献：
    1. expression in offspring bone not changed by maternal high fat diet PMID: 25279792
    2. the uncarboxylated form of osteocalcin does not trigger Akt phosphorylation and glucose uptake by itself but promotes insulin-induced glucose uptake in myotubes, probably by up-regulating Akt signaling through ERK activation PMID: 25735975
    3. The Twist2-Cre, Osterix-Cre and osteocalcin-Cre lines to generate conditional beta1 integrin deletions, were used to investigate the role of beta1 integrins on skeletal phenotype. PMID: 25183373
    4. OCN is gamma-carboxylated by the gamma-carboxylase (GGCX) on three glutamic acid residues, a cellular process requiring reduction of vitamin K by a second enzyme, VKORC1. PMID: 25753038
    5. results showed an increase in the expression of Cbfa1 and OCN in fibroblasts and osteoblasts exposed to fluoride. PMID: 24680482
    6. Data indicate that Ca2+ binding triggered a similar conformational transition in both gamma-carboxyglutamic acid-containing protein osteocalcin (Gla-OCN) and Glu-OCN from a disordered structure to a more compact/stable form. PMID: 24896339
    7. In primary adipocytes, both carboxylated and uncarboxylated osteocalcin increased basal and insulin-stimulated glucose transport. PMID: 24554534
    8. Taken together, these findings demonstrate that SMILE negatively regulates osteocalcin via a direct interaction with RUNX2 PMID: 24389415
    9. Osteoblast-specific expression of Fra-2/AP-1 controls adiponectin and osteocalcin expression and affects metabolism. PMID: 24046454
    10. contribution of OC-OPN to fracture toughness is related to their presence at the extrafibrillar organic-mineral interfaces PMID: 24128197
    11. Insulin resistance in osteoblasts led to a decrease in circulating levels of the active form of osteocalcin, thereby decreasing insulin sensitivity in skeletal muscle. PMID: 24642469
    12. loss of osteocalcin/Gprc6a signaling has a profound effect on beta-cell mass accrual during late pancreas morphogenesis PMID: 24009262
    13. This study reveals that the skeleton via osteocalcin influences cognition and contributes to the maternal influence on fetal brain development. PMID: 24074871
    14. T3 stimulates the activation of Rho-kinase in osteoblasts, which functions as a negative regulator of T3-stimulated osteocalcin synthesis PMID: 23123502
    15. Uncarboxylated osteocalcin acts via Gprc6a to induce GLP-1 release from the gut; the stimulatory effect of uncarboxylated osteocalcin on insulin secretion is largely mediated by GLP-1. PMID: 23437377
    16. These results indicate that PP2A Calpha and its activity play a negative role in osteoblast differentiation and Osterix is a key factor responsible for regulating the expressions of Bsp and OCN during PP2A Calpha-mediated osteoblast differentiation. PMID: 23042641
    17. alphaNAC interacts with histone deacetylase corepressors to control Myogenin and Osteocalcin gene expression. PMID: 23092676
    18. Data from TR4 (steroid/thyroid hormone receptor 4) knockout mice suggest that TR4 binds to promoter region of osteocalcin gene and induces osteocalcin expression; this interaction maintains osteoblast activity during bone development/remodeling. PMID: 22676849
    19. Alterations of responses in knockout mice that suggest participation of osteocalcin in transmission of information about those sensory stimuli. PMID: 22350212
    20. BMP2 induces osteoblast differentiation through Runx2-dependent ATF6 expression, which directly regulates Oc transcription. PMID: 22102412
    21. Data show that Mkp1 KO mice displayed delayed differentiation and reduced expressions of osteocalcin (OCN) and Runx2 genes associated with osteoblast maturation and function. PMID: 21852324
    22. Both BGP overexpression and treatment with purified Bone Gla Protein (BGP) resulted in stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) in chondrocytes and vascular smooth muscle cells PMID: 21757657
    23. Foxo1 is a novel negative regulator of osteoblast-specific transcription factor Runx2 and modulates IGF1/insulin-dependent regulation of osteocalcin expression in osteoblasts. PMID: 21471200
    24. Data show that osteocalcin synthesis was significantly reduced in the stable HSP27-transfected MC3T3-E1 cells and normal human osteoblasts. PMID: 21427224
    25. Findings expand the biological importance of osteocalcin, begin to unravel its molecular mode of action, and provide the first evidence that the skeleton is an endocrine regulator of fertility. PMID: 21333348
    26. the activation of PPARgamma inhibits osteocalcin expression both by suppressing the expression of Runx2 and by interfering with the transactivation ability of Runx2 PMID: 12704187
    27. 1,25-(OH)(2)D(3)and T3 upregulated osteocalcin, depending on the stage of cell differentiation. PMID: 12798773
    28. osteocalcin fibroblast growth factor response element activation is enhanced by MINT, the Msx2 interacting nuclear matrix target PMID: 15131132
    29. Nell-1 reduced osterix-producing cells and increased bone sialoprotein, osteocalcin, and BMP-7 expression. PMID: 16936265
    30. Expression of the osteoblast-specific gene OC was examined in marrow stromal cell cultures at various time points. PMID: 17705049
    31. during osteoblast differentiation, early on, when Runx2 protein and DNA-binding are maximal, Runx2 is nearly absent from the OC promoter. Later, Runx2 is recruited to the OC promoter as Runx2 mRNA, protein, and in vitro DNA binding progressively decrease. PMID: 18821584
    32. Results show that induction of alkaline phosphatase, type I collagen, osteocalcin, CD44, CD47 and CD51 by mevinolin is responsible for the osteoblastic differentiation of D1 cells. PMID: 19162043

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 组织特异性：
    Bone.
  • 数据库链接：

    KEGG: mmu:12096

    STRING: 10090.ENSMUSP00000075425

    UniGene: Mm.389459