Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Pituitary homeobox 2 (Pitx2)

Recombinant Mouse Pituitary homeobox 2 (Pitx2)

  • 货号：
    CSB-YP018043MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018043MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018043MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018043MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018043MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pitx2; Arp1; Brx1; Otlx2; Ptx2; Rgs; Pituitary homeobox 2; ALL1-responsive protein ARP1; BRX1 homeoprotein; Homeobox protein PITX2; Orthodenticle-like homeobox 2; Paired-like homeodomain transcription factor 2; Paired-like homeodomain transcription factor Munc 30; Solurshin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-317
  • 氨基酸序列
    METNCRKLVS ACVQLGVQPA AVECLFSKDS EIKKVEFTDS PKSRKESASS KLFPRQHPGA NEKDKGQQGK NEDVGAEDPS KKKRQRRQRT HFTSQQLQEL EATFQRNRYP DMSTREEIAV WTNLTEARVR VWFKNRRAKW RKRERNQQAE LCKNGFGPQF NGLMQPYDDM YPGYSYNNWA AKGLTSASLS TKSFPFFNSM NVNPLSSQSM FSPPNSISSM SMSSSMVPSA VTGVPGSSLN SLNNLNNLSS PSLNSAVPTP ACPYAPPTPP YVYRDTCNSS LASLRLKAKQ HSSFGYASVQ NPASNLSACQ YAVDRPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform Ptx2c is involved in left-right asymmetry the developing embryo.
  • 基因功能参考文献：
    1. data demonstrate that HTD, but not HTN, can impair Pitx2>>Wnt pathway providing thus a molecular link to AF PMID: 29194452
    2. Defined a transcriptional architecture for atrial rhythm control organized as an incoherent feed-forward loop, driven by TBX5 and modulated by PITX2. TBX5/PITX2 interplay provides tight control of atrial rhythm effector gene expression, and perturbation of the co-regulated network caused atrial fibrillation susceptibility. PMID: 27582060
    3. Pitx2 is essential to maintain iHepSCs stem cell characteristics PMID: 27697592
    4. Study reports that p27 normally exerts a negative feedback on p21 expression: p27 directly represses the expression of the transcription factor Pitx2 which in turn maintains decreased p21 levels. Consequently, in cells lacking p27, de-repression of Pitx2 causes the up-regulation of p21 showing a new mechanism by which p27 regulates cell cycle progression by transcriptionally regulating the expression of Pitx2 and p21. PMID: 27270438
    5. demonstrate a dose-dependent relation between Pitx2 expression and the expression of atrial fibrillation susceptibility genes PMID: 26243430
    6. Data suggest a putative role of the homeobox protein PITX2 (PITX2B) isoform during ventricular septation as well as in the maturation of the right portion of the atrioventricular canal. PMID: 25896281
    7. Pitx2 represses left-side expression of a conserved lncRNA Playrr. Pitx2 auto-regulation directs chromatin topology to coordinate left-right transcription and organogenesis. PMID: 26411685
    8. The AP-2beta transcription factor is an important effector of PITX2 function during corneal development, required for differentiation of corneal endothelium and establishment of angiogenic privilege. PMID: 26968737
    9. Pitx2-deficient neonatal mouse hearts failed to repair after apex resection, whereas adult mouse cardiomyocytes with Pitx2 gain-of-function efficiently regenerated after myocardial infarction PMID: 27251288
    10. This study describes a Pitx2-miRNA pathway controlling cell proliferation in myogenic cells, providing new targets to enhance the regenerative capacity of limb skeletal-muscle myogenic precursor cells for the treatment of skeletal-muscle diseases. PMID: 26055324
    11. de-regulation of both PITX2 and ENPEP could contribute to an increased risk of atrial fibrillation in carriers of disease-associated variants. PMID: 25888893
    12. Pitx2 pathway drives organ-specific arterial and lymphatic development in the intestine PMID: 25482882
    13. Pitx2c may regulate functional Left-Right asymmetry of the developing limb. PMID: 25224222
    14. The combination of Pitx2, a regulator of dental stem cells and miR-200a converts mesenchymal cells to a fully differentiated dental epithelial cell type. PMID: 25122764
    15. Pitx2 has genetically separable postnatal and developmental functions, unveil direct Pitx2 target genes that include channel and calcium handling genes, as well as genes that stabilize the intercalated disc in postnatal atrium. PMID: 24395921
    16. in normal tooth development Pitx2 activates amelogenin expression, whose product is required for enamel formation; this regulation is perturbed by missense PITX2 mutations in Axenfeld-Rieger Syndrome (ARS); findings define transcriptional mechanisms involved in normal tooth development and shed light on molecular underpinnings of enamel defect in ARS PMID: 23975681
    17. Pitx2 regulates developmental gut rotation via Daam2 activation and asymmetric Wnt signaling. PMID: 24091014
    18. Pitx2 influences the expression of transcription factors and signaling molecules involved in the differentiation of the cushion mesenchyme during heart development. PMID: 23361844
    19. Pitx2 is highly expressed in the incisor cervical loop stem cell niche. PMID: 23863486
    20. the differential expression of Pitx2 between EOM and limb skeletal muscle suggest a role for Pitx2 in the maintenance of constitutive differences between EOM and limb skeletal muscle that may contribute to the sparing of EOM in muscular dystrophies. PMID: 23505501
    21. These results provide insights into Pitx2 dosage and isoform-specific requirements underlying midbrain and hypothalamic development. PMID: 23147109
    22. Transient transfections demonstrate the inhibitory effect of Dact2 on critical dental epithelial differentiation factors during tooth development. Dact2 significantly inhibits PITX2 activation of the Dlx2 and amelogenin promoters. PMID: 23349981
    23. Piasy represses the synergistic activation of PITX2 with interacting co-factors and Piasy represses Pias1 activation of PITX2 transcriptional activity. PMID: 23515314
    24. the PITX2 and Wnt pathway exerts a positive feedback regulation, whereas frizzled receptors generate a negative feedback in this pathway. PMID: 23250740
    25. Using tissue specific Fgf9 versus Pitx2 loss of function approaches in the gut epithelium and/or mesenchyme, we determined that FGF9 signals to the mesenchyme via Pitx2 to induce mesenchymal Fgf10 expression, which leads to epithelial cecal bud formation. PMID: 22819677
    26. Pitx2 appears to regulate muscle anlagen development by appropriately balancing expression of cytoskeletal and adhesion molecules. PMID: 22558231
    27. Pitx2 primarily regulates a relatively select number of genes associated with muscle contraction. PMID: 22408009
    28. Pitx2 plays a crucial functional role by negatively modulating a nodal-type programme in the left sinus venosus myocardium. PMID: 22116619
    29. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. PMID: 21727215
    30. PITX2 marks a distinct population of GABAergic neurons in mouse embryonic ventral hindbrain rhombomere 1. This population appears to retain its GABAergic identity even in the absence of PITX2. PMID: 21925604
    31. TGFbeta-FGF9-PITX2 signaling cascade regulates cranial neural crest cell proliferation during palate formation. PMID: 22123828
    32. Overexpression of Pitx2c in embryonic stem cell-derived cardiomyocytes is able to dynamically upregulate several cardiac-enriched transcription factors such as Isl1, Mef2c and Gata4. PMID: 21389672
    33. Pitx2 overexpression may play an antiapoptotic role during Nonfunctioning pituitary adenomas tumorigenesis. PMID: 21810944
    34. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis PMID: 21726547
    35. Pitx2c modulates Pax3 by repressing miR27 expression and that Pax3-miR-27 modulation mediated by Pitx2c is independent of Pitx2c effects on cell proliferation PMID: 21749861
    36. suggest that Pitx2 is required for initiation of mesodermal budding and likely resides upstream of Fgf10-Fgfr2IIIb signaling in the normal development of this structure PMID: 21550054
    37. Results identify Pitx2 as an upstream transcriptional regulator of atrial electric function, the insufficiency of which results in cellular and molecular changes leading to atrial electric and structural remodeling linked to arrhythmogenesis. PMID: 21511879
    38. Characterization of Pitx2c expression in the mouse heart using a reporter transgene. PMID: 21089073
    39. Data suggest that PITX2 is present in regionally restricted subpopulations of midbrain neurons and may have unique functions that promote GABAergic and glutamatergic differentiation. PMID: 21246650
    40. Findings demonstrate that the overactivated PITX2-Cyclin D2 pathway promotes thyroid tumorigenesis. PMID: 20372070
    41. Study propose a model wherein a sequential code of retinoic acid followed by canonical Wnt signaling are required for activation and maintenance of Pitx2 expression, respectively. PMID: 20960542
    42. Pitx2 is an upstream activator of extraocular myogenesis and survival. PMID: 21035439
    43. Data from these studies suggest that Pitx2 acts as an inhibitor of protein transport and cell apoptosis contributing to the open body wall phenotype. PMID: 20797427
    44. Tbx1(-/+)/Pitx2(-/+) double heterozygous mice present with an extra premolar-like tooth revealing a genetic interaction between these factors. The ability of Tbx1 to repress PITX2 activation of p21 may promote cell proliferation. PMID: 20816801
    45. we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells in the spinal cord PMID: 20437528
    46. findings implicate Pitx2 and Pitx2-mediated left-right asymmetry-signaling pathways in prevention of atrial arrhythmias PMID: 20457925
    47. Pitx2-dependent occupancy by histone deacetylases is associated with T-box gene regulation in mammalian abdominal tissue PMID: 20129917
    48. the presumptive secondary heart field, derived from branchial arch and splanchnic mesoderm, patterns the forming outflow tract and reveal a role for Pitx2c in aortic arch remodeling PMID: 12397115
    49. PITX2 expression in postmitotic neurons may contribute to development of GABAergic and other differentiated neuronal phenotypes PMID: 12453462
    50. Pitx2 is rapidly induced by the Wnt/Dvl/beta-catenin pathway and is required for effective cell-type specific proliferation by directly activating specific growth-regulating genes. PitX2 is converted from a repressor to an activator. PMID: 12464179

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family, Bicoid subfamily
  • 组织特异性：
    In day-11 embryos, expressed in the periocular mesenchyme, maxillary and mandibular epithelia, umbilicus, Rathke pouch, vitelline vessels and limb mesenchyme. In adult tissues, expressed in pituitary gland, brain, kidney, eye, lung, testis and tongue.
  • 数据库链接：

    KEGG: mmu:18741

    STRING: 10090.ENSMUSP00000047359

    UniGene: Mm.246804