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  4. Recombinant Penicillium chrysogenum Ubiquitin-like protein ATG12 (atg12)

Recombinant Penicillium chrysogenum Ubiquitin-like protein ATG12 (atg12)

  • 货号：
    CSB-YP002283ETH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002283ETH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002283ETH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002283ETH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002283ETH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    atg12; Pc12g08030Ubiquitin-like protein ATG12; Autophagy-related protein 12
  • 种属：
    Penicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-172
  • 氨基酸序列
    MNTSSPTRSP QSSPSPNPQS TLSHRPSPRQ PQSSDTPPNS SANAPIPDDE HGADLPMNMT ASVMLTNLPR DAHQALADVE SIDSGKVTVR FQPLPSAPIL KNRVFKVSAS QKFETVVKFL RKKLDCKDTD SVFCYVNSVF APGLDEGMGG LWRCFKTDDQ LIVAYSMTPA FG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with atg5 through a ubiquitin-like conjugating system involving also atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-atg5 conjugate functions as an E3-like enzyme which is required for lipidation of atg8 and atg8 association to the vesicle membranes.
  • 亚细胞定位：
    Preautophagosomal structure membrane; Peripheral membrane protein.
  • 蛋白家族：
    ATG12 family
  • 数据库链接：

    KEGG: pcs:Pc12g08030

    STRING: 500485.XP_002557636.1