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  4. Recombinant Rat Brain-derived neurotrophic factor (Bdnf)

Recombinant Rat Brain-derived neurotrophic factor (Bdnf)

  • 货号：
    CSB-YP002655RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002655RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002655RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002655RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002655RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BdnfBrain-derived neurotrophic factor; BDNF) [Cleaved into: BDNF precursor form; ProBDNF)]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    131-249
  • 氨基酸序列
    HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
  • 基因功能参考文献：
    1. data highlight the role of miR-206 as a post-transcriptional inhibitor of BDNF at E13 in pregnant hypothyroid rats. PMID: 30119135
    2. BDNF inhibits the apoptosis of cardiomyocytes induced by Ang II, and it plays the role by inhibiting CaMK2 and CaN signaling pathways. PMID: 29773102
    3. The above results suggest that rutin preconditioning ameliorates cerebral I/R injury in OVX rats through ER-mediated BDNF-TrkB and NGF-TrkA signaling. PMID: 29420916
    4. BDNF levels were much higher in both the right and left motor cortices of the mild traumatic brain injury group compared to medial prefrontal cortex, which is relatively remote from the impact site PMID: 29648487
    5. TRPA1-mediated spontaneous [Ca(2+)]i increase modulates the spontaneous release of peptide hormones from astrocytes. PMID: 29777700
    6. Results indicate the BDNF system (precursor proBDNF, BDNF pro-peptide, BDNF mRNA levels, as well as TrkB and p75(NTR) receptors) in the brain of rats selectively bred for more than 85 generations for either the high level or the lack of fear-induced aggressive is extremely different at all levels, from transcription to reception, suggesting role of BDNF system in the development of highly aggressive phenotype. PMID: 29425916
    7. Exercise training with concomitant nitric oxide synthase inhibition improved anxiogenic behavior and spatial cognition by enhancement of BDNF and P70S6K activity in the hippocampus and cortex. PMID: 28854337
    8. The environmental enrichment protected against the effects of maternal separation as indicated by the lower anxiety levels and the reversal of spatial memory deficits compared to animals housed in standard conditions. These changes were associated with increased BDNF expression in the hippocampus PMID: 29545098
    9. that BDNF activates mTOR to up-regulate NR2B expression, which is required for inflammatory pain-related aversion in the rostral anterior cingulate cortex of rats PMID: 29353374
    10. Data indicate that brain-derived neurotrophic factor confers neuroprotective effects against irradiation by modulating the NFATc4/3 pathway. PMID: 29288061
    11. McGill APP transgenic rats exhibit differential dysregulation in NGF and BDNF neurotrophins. At early stages, intraneuronal Abeta leads to significant reductions in BDNF mRNA expression, which correlate with learning and memory deficits. At later stages, deficits in the NGF metabolic pathway could explain the paradoxical upregulation of proNGF in the absence of changes in NGF mRNA. PMID: 28865749
    12. findings suggested that BDNF contributed to neuronal growth and proliferation and differentiation of Neural Sstem Cellss in vitro by stimulating PI3K/AKT/GSK3beta/beta-catenin pathways. PMID: 28524001
    13. Results suggest that tongue motor loss impairs hippocampus-dependent cognitive function, and decreased BDNF expression in the hippocampus may be implicated in its underlying molecular mechanism. PMID: 28767193
    14. Cerebral hemorrhage significantly inhibited the spatial learning and memory ability of rats; the mechanism may be related to decreased cerebral expression of BDNF and NGB PMID: 28829495
    15. Reduced Bdnf/trkB signaling in the hippocampus of vulnerability (Roman low-avoidance) and resistance (Roman high-avoidance) rats may contribute to their more pronounced vulnerability to Stress-induced Depression. PMID: 29075579
    16. BDNF-induced phrenic motor facilitation requires downstream signaling via PKCtheta; but not MEK/ERK or PI3K/Akt signaling. These data are essential to understand the sequence of the cellular cascade leading to BDNF-dependent phrenic motor plasticity. PMID: 28855298
    17. Results indicate that the hippocampal brain-derived neurotrophic factor/TrkB signaling pathway is activated by multiple electroconvulsive seizure treatments despite the ligand-induced down-regulation of the full-length TrkB receptor. PMID: 28890399
    18. These findings will contribute to the development of a novel therapeutic strategy for peripheral nerve injury, which overcomes the limitations of direct administration of exogenous BDNF by using miR-1 to regulate endogenous BDNF expression. PMID: 27381812
    19. Data suggest that DNA methylation of BDNF exon IX is significantly increased in hippocampal neurons in zinc deficient animals as compared to control animals; expression of BDNF mRNA is decreased. PMID: 27329329
    20. This study demonstrated that a long-term impact of prenatal stress on brain BDNF DNA methylation and telomere biology with relevance for behavioral and health outcomes. PMID: 28330827
    21. Results suggest that the DNA methyltransferase-mediated DNA methylation at the BDNF promoters IVp and IXp might be involved in the regulation of BDNF gene expression in the amygdala. Further, it could also be related to minimal traumatic brain injury-induced anxiety-like behaviors via the regulation of synaptic plasticity. PMID: 28663119
    22. This study demonstrated that Aberrant gene expression of brain derived neurotrophic factor (Bdnf) in the hippocampus (HPC)-prefrontal cortex (PFC) network was observed in binge eating prone rats. PMID: 28821448
    23. these data suggest at CpG sites of the bdnf promoter, SNL-enhanced Tet1 expression promotes DNA demethylation both by converting 5 mC to 5 hmC and inhibiting DNMT binding to regulate spinal BDNF expression, hence contributing to behavioral allodynia development. PMID: 27857218
    24. Treadmill running protected against IFN-alpha induced depression decreases in expression of BDNF in the hippocampus and prefrontal cortex. Aerobic exercise protects against IFN-alpha induced affective and cognitive dysfunction, which is associated with increased BDNF. PMID: 28711394
    25. BDNF regulates the unfolded protein response via TrkB-linked JNK inactivation in the dorsal striatum after repeated cocaine administration, thus contributing to the restoration of the endoplasmic reticulum functions. PMID: 28238708
    26. BDNF protects the heart against septic cardiac dysfunction by reducing oxidative stress and apoptosis via Trk-B, and it does so through activation of eNOS/NO pathway. These findings provide a new treatment strategy for sepsis-induced myocardial dysfunction. PMID: 28770018
    27. Study found that the BDNF propeptide promoted C6 glioma cell apoptosis and decreased in-vitro cell growth. Study also found using western blot that cleaved caspase3 and B cell lymphoma 2 (Bcl2)-associated X protein abundances increased, whereas Bcl2 abundance decreased. The data suggest that the BDNF propeptide may have an inhibitory effect on glioma through activation of the caspase3 pathway. PMID: 28640008
    28. BDNF participates in the regulation of the dopamine system by acting on DRD3 and DAT. PMID: 28598964
    29. The results showed that LIPUS enhanced the Expression of Brain-Derived Neurotrophic Factor in Astrocyte Through Activation of TrkB-Akt and Calcium-CaMK signaling pathway. PMID: 27252349
    30. This study demonstrated that endurance training increased the BDNF in brain. PMID: 28445707
    31. These studies demonstrate the long-lasting negative impact of developmental alcohol exposure on hippocampal dendritic morphology and Bdnf gene expression support the implementation of exercise and complex environments PMID: 27597545
    32. Studies findings reveal that brain-derived neurotrophic factor has direct GABAA receptor independent effects on hypothalamic paraventricular nucleus neurones, which are modulated by local glucose concentrations. PMID: 28258626
    33. the decrease in BDNF secretion and the BDNF expression might depend on the disruption of microtubule structure caused by tau hyperphosphorylation PMID: 27914953
    34. These data suggest that methylation change in BDNF exon is involved in the regulation of BDNF expression by Abeta or SAM, and further support the view of specific epigenetic modifications of a certain BDNF gene transcript. PMID: 29305263
    35. Enhanced neuroprotective efficacy of bone marrow mesenchymal stem cells co-overexpressing BDNF and VEGF in a rat model of cardiac arrest-induced global cerebral ischemia. PMID: 28492549
    36. Regarding BDNF, the isolation-reared rats demonstrated increased BDNF mRNA levels, H3 acetylation at the BDNF gene and BDNF protein expression in the medial prefrontal cortex PMID: 27435421
    37. in exposure to a stressor, sex differences in BDNF methylation may be root cause of decreased BDNF levels in females and may underlie susceptibility to pathology development. PMID: 26189832
    38. Study found that spinal microglia were activated and that P2X4R was primarily expressed in spinal activated microglia during nicotine withdrawal (NTW). In addition, the P2X4R and BDNF expression levels in the spinal cord were upregulated during NTW. Furthermore, inhibition of microglial activation prevented thermal hyperalgesia and increased P2X4R and BDNF expression induced by NTW. PMID: 28306606
    39. Brain-derived neurotrophic factor (BDNF) immunocontent was increased in the hippocampus of male rats receiving tactile stimulation (TS) from postnatal day (PND) 8-14, while glucocorticoid receptors immunocontent was decreased in both TS1-7 and TS15-21 , but not TS8-14; TS is more efficient if applied over a focused period of neonatal development (PND8-14) and can reduce emotionality and increase stress coping in adulthood PMID: 27874237
    40. Following IL-1beta (50mug/kg ip.) administration, a higher serum IL-1beta level was detected in females than in males. In male rats, IL-1beta decreased BDNF mRNA in all the brain structures, except for the pituitary, and VEGF mRNA in the amygdala. PMID: 27744091
    41. BDNF heterozygosity results in marked deficits in object and fear memory, as well as male-specific alterations in cortical and hippocampal EEG activity. These changes in EEG spectral power may indicate a differential mechanism of learning and memory deficits between male and female BDNF knock-out rats. PMID: 28576309
    42. exposure to an acute stress upregulates Bdnf expression in the prefrontal cortex which is selectively impaired in prenatal stress female rats, an effect that was associated with changes in the modulation of the DNA demethylase Gadd45beta. PMID: 26676568
    43. Study microinjected BDNF followed by AMPA (or antagonists) into the commissural nucleus tractus solitarius (cNTS), before application of NaCN into the carotid body receptors (CBR), and measured changes in glucose concentrations in arterial and venous blood. Results show that BDNF prevented the inhibitory effects that AMPA injections have in cNTS on the glycemic response induced by the local application of NaCN in CBR. PMID: 28254195
    44. these observations demonstrated the detailed functional interaction between BDNF and mGluR II: Activation of mGluR II positively regulates self-induced BDNF expression, and, in turn, BDNF negatively regulates the gene expression of mGluR II in a neuronal activity-independent manner, in cortical neurons, but not in astrocytes. PMID: 28119091
    45. We find that BDNF mediates NMDAR potentiation through activation of TrkB and phosphorylation of the GluN2B subunit by the Src-family kinase Fyn PMID: 27926876
    46. Ouabain decreased sensitivity to negative feedback during the initial phase of reversal learning. Expression of BDNF mRNA and protein levels was downregulated in the frontal cortex which also negatively correlated with regressive errors. Findings suggest that the ouabain model of mania may be useful in understanding the neuropathophysiology that contributes to cognitive flexibility deficits and test potential treatments. PMID: 27267245
    47. Neurogenesis and BDNF levels were reduced in isolated and morphine-treated isolated rats as compared to group-housed rats and morphine-treated group-housed rats, respectively. PMID: 27550421
    48. PSD-95 mediates the dendrite arborization-promoting effect of BDNF. PMID: 28698933
    49. BDNF pro-peptide regulates dendritic spines via CASP3. PMID: 27310873
    50. results indicate that adolescent intermittent ethanol exposure increases hippocampal histone deacetylase and decreases cyclic adenosine monophosphate response element binding protein levels that may be associated with a decrease in histone H3 acetylation in the hippocampus. These epigenetic changes potentially decrease brain-derived neurotrophic factor expression and inhibit neurogenesis in the hippocampus. PMID: 26941165

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  • 亚细胞定位：
    Secreted.; [BDNF precursor form]: Secreted.
  • 蛋白家族：
    NGF-beta family
  • 数据库链接：

    KEGG: rno:24225

    STRING: 10116.ENSRNOP00000064356

    UniGene: Rn.11266