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  4. Recombinant Rat Calcium/calmodulin-dependent protein kinase type II subunit beta (Camk2b)

Recombinant Rat Calcium/calmodulin-dependent protein kinase type II subunit beta (Camk2b)

  • 货号：
    CSB-YP004466RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004466RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004466RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004466RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004466RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Camk2b; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; EC 2.7.11.17
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-542
  • 氨基酸序列
    MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKL SARDHQKLER EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI LEAVLHCHQM GVVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKEA YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLIN QMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKL KGAILTTMLA TRNFSVGRQT TAPATMSTAA SGTTMGLVEQ AKSLLNKKAD GVKPQTNSTK NSSAITSPKG SLPPAALEPQ TTVIHNPVDG IKESSDSTNT TIEDEDAKAR KQEIIKTTEQ LIEAVNNGDF EAYAKICDPG LTSFEPEALG NLVEGMDFHR FYFENLLAKN SKPIHTTILN PHVHVIGEDA ACIAYIRLTQ YIDGQGRPRT SQSEETRVWH RPDGKWQNVH FHCSGAPVAP LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
  • 基因功能参考文献：
    1. VLDLR requires RasGRF1/CaMKII to alter dendritic spine formation. PMID: 25644714
    2. Results suggest that a sodium-dependent glutamate transporter-CaMKIIbeta-actin cytoskeleton axis plays an important role in the regulation of oligodendrocyte maturation and central nervous system myelination PMID: 24866099
    3. BDNF-induced CaMKIIbeta phosphorylation and premature synapse formation were suppressed in GIT1 (S419A) transfected cortical neurons PMID: 23352984
    4. These results identify betaCaMKII as a powerful regulator of lateral habenula neuron function and a key molecular determinant of depression. PMID: 23990563
    5. Study shows targeting of Arc to inactive synapses via a high-affinity interaction with CaMKIIbeta that is not bound to calmodulin. PMID: 22579289
    6. Activation of intracellular CaMKII signaling in the pedunculopontine tegmental nucleus promotes wakefulness and suppresses sleep. PMID: 22114270
    7. The beta isoenzyme of Ca(2+)/calmodulin-dependent kinase type II as possible mediator of somatostatin functions in pituitary tumour cells PMID: 21952434
    8. play distinct role during the development of the retina. PMID: 12234658
    9. Selective regulation of neurite extension and synapse formation by the beta but not the alpha isoform of CaMKII. PMID: 12873385
    10. Activities of both enzymes increased upon their association, and iPLA(2)beta reaction products reduced CaMKIIbeta activity PMID: 15576376
    11. CaMKIIbeta binding to NR2B C-tail was examined. Translocation was observed for CAMKIIbeta. Ionomycin dispersed CaMKIIbeta into a punctate pattern. PMID: 16436603
    12. F-actin-targeted CaMKIIbeta, but not alpha, was able to phosphorylate actin in vitro even by nonstimulated basal activity. PMID: 16928958
    13. Study suggest that Camk2a is not involved in regulating the acute ethanol sensitivity of NMDA receptors. PMID: 18562151
    14. endogenous CaMKIIbeta, but not CaMKIIalpha, localized to prominent F-actin-rich structures at the soma in embryonic cortical neurons. PMID: 18840684
    15. CaMKII locally encodes L-type channel activity to signal to nuclear CREB in excitation-transcription coupling. PMID: 19047462

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  • 亚细胞定位：
    Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with the cortical actin cytoskeleton by binding to F-actin in hippocampal cells.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
  • 数据库链接：

    KEGG: rno:24245

    UniGene: Rn.9743