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  4. Recombinant Rat Mitogen-activated protein kinase 3 (Mapk3)

Recombinant Rat Mitogen-activated protein kinase 3 (Mapk3)

  • 货号：
    CSB-YP013456RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013456RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013456RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013456RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013456RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; EC 2.7.11.24; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-380
  • 氨基酸序列
    AAAAAAPGG GGGEPRGTAG VVPVVPGEVE VVKGQPFDVG PRYTQLQYIG EGAYGMVSSA YDHVRKTRVA IKKISPFEHQ TYCQRTLREI QILLRFRHEN VIGIRDILRA PTLEAMRDVY IVQDLMETDL YKLLKSQQLS NDHICYFLYQ ILRGLKYIHS ANVLHRDLKP SNLLINTTCD LKICDFGLAR IADPEHDHTG FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML SNRPIFPGKH YLDQLNHILG ILGSPSQEDL NCIINMKARN YLQSLPSKTK VAWAKLFPKS DSKALDLLDR MLTFNPNKRI TVEEALAHPY LEQYYDPTDE PVAEEPFTFD MELDDLPKER LKELIFQETA RFQPGAPEAP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
  • 基因功能参考文献：
    1. Retinoic acid was also found to rapidly downregulate extracellular signal-regulated kinase (ERK) 1/2 phosphorylation. PMID: 29520716
    2. Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID: 29742816
    3. Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction. PMID: 29901080
    4. Signal communication between neurons and satellite glial cells expands the duration of neuronal ERK1/2 phosphorylation and pain hypersensitivity at 1 h after tissue injury. PMID: 29592783
    5. The experimental results demonstrated that there was a coexistence of GRs and pERK 1/2 in the spinal cord dorsal horn by double immunofluorescence staining. The GR antagonist RU38486 attenuated the morphine analgesia tolerance by inhibiting the expression of GR and increasing the expression of pERK. PMID: 29845273
    6. miRNA-15b deteriorates cardiomyocyte apoptosis by post-transcriptionally downregulating the expression of Bcl-2 and MAPK3. PMID: 28814571
    7. ERK1/2 and Akt phosphorylation were essential for mechano-growth factor E peptide regulating cell morphology and mobility but not proangiogenic capacity of bone marrow-derived mesenchymal stem cells under severe hypoxia PMID: 29441602
    8. Study shows that the mitogen-activated protein kinase pathway is involved in promoting the sodium appetite after low-sodium diet consumption. PMID: 28836382
    9. Homocysteine up-regulates ETA receptor expression via the Sirt1/ERK1/2 signaling pathway in vascular smooth muscle cells. PMID: 28579512
    10. Study demonstrated that blockade of audiogenic seizure (AGS) by inactivation of ERK1/2 inhibits Synapsin I activity and glutamate release in the striatum, decreases the activity of dopaminergic neurons, and leads to upregulation of GABA synthesis in the substantia nigra; suggests that nigrastriatal system may be involved in or affected by the AGS expression in Krushinsky-Molodkina rats. PMID: 28738742
    11. Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway. PMID: 28758954
    12. Phosphorylated extracellular signal-regulated kinase 1/2 blockade in the Locus Coeruleus relieved anxiety and counteracted altered Locus Coeruleus function; s propose that phosphorylated extracellular signal-regulated kinase 1/2 activation in the Locus Coeruleus plays a crucial role in pain-related anxiety. PMID: 28158734
    13. Apocynin may act as a novel molecular candidate to protect against vascular smooth muscle cell osteogenic switching/vascular calcification through suppressing ERK1/2 pathway. PMID: 27835878
    14. miR-15b-5p might inhibit cell proliferation and induce apoptosis in PC12 cells by targeting ERK1 expression. PMID: 28609839
    15. Late pregnancy is a critical period for changes in phosphorylated mitogen-activated protein kinase/extracellular signal-regulated kinase 1/2 in oxytocin neurones. Results suggest an important role for the MAPK/ERK pathway during reproductive changes in the supraoptic and paraventricular nucleus. PMID: 27203238
    16. EMF-induced cyclic adenosine monophosphate (cAMP) level increase causes protein kinase A (PKA) and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. PMID: 24634418
    17. ERK1/2 kinases in the inferior colliculus of rats genetically prone to audiogenic seizures during postnatal development PMID: 29101749
    18. DOX induced mitochondrial ROS release to activate ERK1/2-mediated HSF2 nuclear translocation and AT1 R upregulation causing DOX-damaged heart failure in vitro and in vivo. PMID: 28295305
    19. CTRP9 regulates hypoxia-mediated human pulmonary artery smooth muscle cell proliferation, apoptosis and migration via TGF-beta1/ERK1/ERK2 signaling pathway. PMID: 28688765
    20. Inhibiting miR-21 attenuates hepatic fibrosis by suppressing both the ERK1 in hepatic stellate cells and epithelial-mesenchymal transition of hepatocyte. PMID: 27226339
    21. Astrocytes can cause Ca(2+) signaling activation, ERK1/2 phosphorylation, and Id4 expression stimulation in oligodendrocyte progenitor cells inducing proliferation of these cells, mainly through Cx47. PMID: 28278052
    22. Neuroprotective mechanism of sevoflurane pre-conditioning might be associated with increased Erk1/2 phosphorylation in mitochondria via inhibition of mPTP opening in the early reperfusion period PMID: 28017206
    23. Rap guanine nucleotide exchange factors Epac1 and Epac2 induces the phosphorylation of extracellular signal-regulated kinases ERK1/2 in pituitary tumor GH3 cells. PMID: 28216156
    24. Alisol A 24-acetate effectively reverses the phenotypic transformation and inhibits the migration of vascular smooth muscle cells, which may be associated with the suppression of the ERK1/2 signaling pathway. PMID: 27924795
    25. Results demonstrated that uric acid can stimulate p38 MAPK, p44/42 MAPK and PDGFRbeta phosphorylation in a time- and concentration-dependent manner. Furthermore, treatment of VSMCs with the angiotensin II type I receptor (AT1R) inhibitor losartan suppressed p38 MAPK and p44/42 MAPK induction by uric acid. PMID: 27400779
    26. Enriched environment could promote rehabilitation after cerebral ischemia via regulation of p-ERK1/2 expression. PMID: 27068861
    27. Paridis rhizoma sapoinins attenuate CCl4-induced liver fibrosis through the regulation of RASAL1/ERK1/2 signal pathway. PMID: 27396351
    28. Data indicate that mitogen activated protein kinases c-Jun N-terminal kinase, p38, and ERK1/2 were activated but with different distributing patterns in the placenta. PMID: 26491907
    29. Data show that N-acetylcysteine modulates mitogen activated protein kinase s ERK1/2 and NF-kappa B signaling pathways in soleus muscle of heart failure (HF). PMID: 27351177
    30. Bisphenol A may promote proliferation of and collagen production by cardiac fibroblasts via activation ERK1/2 signaling pathway. PMID: 27025157
    31. MEK-ERK1-2 pathway is involved in regulating the anti-oxidant strategies to compensate the oxidative status induced by diesel exhaust particle exposure. PMID: 27091075
    32. Erk1 can be considered a proto-oncogene and that Erk molecules possess unusual autoregulatory properties, some of them independent of TEY phosphorylation. PMID: 26658610
    33. The ERK1 in the insular cortex as a part of pathophysiological process that determines predisposition of Krushinsky-Molodkina rats to audiogenic seizures. PMID: 26923581
    34. Beta1 integrin inhibits apoptosis induced by cyclic stretch in annulus fibrosus cells via ERK1/2 MAPK pathway. PMID: 26467923
    35. Surviving CA1 pyramidal cells were identified by hematoxylin and eosin staining and the levels of phosphorylated ERK1/2, P38MAPK and JNK were detected by western blotting. PMID: 26549199
    36. CaSR might play significant roles in the induction of hippocampus apoptosis in rat during hypoxia/reoxygenation through phosphorylation of ERK1/2. PMID: 26617793
    37. TGF-beta1 antagonizes TNF-alpha induced up-regulation of matrix metalloproteinase 3 in nucleus pulposus cells: role of the ERK1/2 pathway. PMID: 26075533
    38. The effects of combinative early and delayed ischemic postconditioning on producing brain BDNF in ischemia may be mediated by the activation of ERK1/2 and CREB. PMID: 26398857
    39. it was shown that HCQ is protective via enhanced phosphorylation of the pro-survival kinase ERK1/2. PMID: 26636577
    40. This study showed that sharp increases in phosphorylated or activated ERK1 was observed at postnatal day (PND) 7 in the SN and VTA. In contrast, in the STR, the levels of phosphorylated ERK1 was significantly higher at postnatal 0 day. PMID: 26363522
    41. inhibition of ERK1/2 activation has no therapeutic effect on kidney fibrosis in SNx possibly due to increased compensatory activation of the p38 and JNK signalling pathways with subsequent upregulation of PAI-1. PMID: 26415098
    42. overexpression of ERp29 in cortical neurons after axotomy increased expression of Erk-1 and PI3K while decreasing the expression of caspase-3 expression. PMID: 25204493
    43. in the native GH3 cell system, ERK and RSK are involved in the pathway linking TRH receptor to r-ERG channel inhibition, but additional components must participate to cause such inhibition. PMID: 26022182
    44. The expression of Cx43 contributes to aldosterone-induced mesangial cell proliferation via the ERK1/2 and protein kinase C pathways. PMID: 26138882
    45. Data show that the simultaneous presence of G-protein-coupled receptor 30 (GPR30) and estrogen receptor alpha (ERalpha) is required for the action of peptide ERalpha17p activation of ERK1/2 (extracellular-signal-regulated kinase 1/2). PMID: 26371374
    46. Enhanced NOX4 expression results in cardiac fibrosis through reactive oxygen species-ERK1/2-MAP kinase-dependent mechanisms in diabetic cardiomyopathy. PMID: 26088607
    47. ERK1/ERK2 activity mainly in the glutamategic neurons of the hippocampus and the temporal cortex is a positive regulator of glutamate release that results in the initiation of auditory seizures. PMID: 26827493
    48. The expression of DUSP6 and DUSP7 is mediated by ERK1/ERK2 activity in microglia. PMID: 26435497
    49. both AKT and ERK1/2 pathways are crucial for the carotid body intracellular signaling process in response to hypoxia. PMID: 26303491
    50. The results are indicative of a novel role of ERK1/2 in glucose-regulated POMC expression and offer new mechanistic insights into hypothalamic glucose sensing. PMID: 25624461

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane, caveola. Cell junction, focal adhesion.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 组织特异性：
    Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.
  • 数据库链接：

    KEGG: rno:50689

    STRING: 10116.ENSRNOP00000026627

    UniGene: Rn.2592