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  4. Recombinant Rat RAC-alpha serine/threonine-protein kinase (Akt1)

Recombinant Rat RAC-alpha serine/threonine-protein kinase (Akt1)

  • 货号：
    CSB-YP001553RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001553RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001553RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001553RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Akt1RAC-alpha serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-480
  • 氨基酸序列
    MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVEQRES PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KRQEEETMDF RSGSPSDNSG AEEMEVALAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPTQ RLGGGSEDAK EIMQHRFFAN IVWQDVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53. Phosphorylates palladin (PALLD), modulating cytoskeletal organization and cell motility. Phosphorylates prohibitin (PHB), playing an important role in cell metabolism and proliferation. Phosphorylates CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation. Phosphorylates PCK1 at 'Ser-90', reducing the binding affinity of PCK1 to oxaloacetate and changing PCK1 into an atypical protein kinase activity using GTP as donor. Also acts as an activator of TMEM175 potassium channel activity in response to growth factors: forms the lysoK(GF) complex together with TMEM175 and acts by promoting TMEM175 channel activation, independently of its protein kinase activity.
  • 基因功能参考文献：
    1. Estradiol up-regulates Cav1.2alpha1C and ICa,L via plasma membrane ER and by activating Pi3K, Akt, and CREB signaling. The promoter regions of the CACNA1C gene (human-rabbit-rat) contain adjacent/overlapping binding sites for p-CREB and ERalpha, which suggests a synergistic regulation by these pathways. PMID: 29330129
    2. AS-IV evoked Akt phosphorylation, and subsequent induced phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) at Ser9 (that is, inactivation), which stimulated Nrf2-mediated antioxidant response element (ARE)-containing activation. PMID: 28315454
    3. these findings reveal that SC79 could accelerate the recovery of reversible muscular atrophy induced by BTX and subsequently promote fracture healing through activation of the Akt signaling pathway, which suggests its therapeutic potential in orthopedics. PMID: 28778592
    4. EPO potentiates the ability of miR-130b to activate Akt and augment apoptosis resistance. Our findings identify the apoptosis-resistant function of miR-130b and suggest that histone H3 deacetylation plays a pivotal role in regulating miR-130b transcription in response to 6-OHDA. PMID: 28412322
    5. Low AKT1 expression is associated with intestinal ischemia/reperfusion injury. PMID: 30195035
    6. Dysregulation of PI3K-Akt-mTOR pathway in the brain of streptozotocin-induced type 2 diabetes mellitus rats. PMID: 30041644
    7. Cardiac progenitor cellderived exosomes promoted H9C2 cell growth via the activation of Akt/mTOR. PMID: 29786755
    8. The PI3K/mTOR/AKT pathway is known to regulate cell growth, survival, and proliferation and has more recently been shown to be a central mediator of HCC cell survival to heat stress. PMID: 29737948
    9. these results suggest that tacrolimus may protect rats from MIRI through activation of the PPARgamma/PI3K/Akt pathway. PMID: 29488613
    10. results suggested that PTE may promote BMSC proliferation and osteogenic differentiation via a mechanism associated with the regulation of Let-7f-5p and the TNFR2/PI3K/AKT signaling pathway. PMID: 29975930
    11. the lower expression levels of CXCR4 and p-AKT may be responsible for the impaired osteogenic ability and lower chemotactic activity towards SDF-1alpha of OVX-bone marrow mesenchymal stem cells . PMID: 29207050
    12. we demonstrated that ARQ 092 blocks AKT phosphorylation in vitro and in vivo In the Hepatocellular carcinoma (HCC)-rat model, ARQ 092 was well tolerated, showed antifibrotic effect, and had stronger antitumor effect than sorafenib. Our results confirm the importance of targeting AKT in HCC. PMID: 28566435
    13. CPT exhibited a protective effect against cerebral stroke through inhibition of the PI3K/AKTeNOS signaling pathway. These results suggested the potential of CPT as a promising agent in the treatment of cerebral stroke. PMID: 29152647
    14. The PI3K/AKT pathway in the nucleus accumbens (NAc) of rodents plays a role in the molecular and behavioral adaptations that are induced by alcohol, and that in turn drives alcohol-drinking behaviors. PMID: 27766766
    15. ERK1/2 and Akt phosphorylation were essential for mechano-growth factor E peptide regulating cell morphology and mobility but not proangiogenic capacity of bone marrow-derived mesenchymal stem cells under severe hypoxia PMID: 29441602
    16. Target of Akt1 is necessary but not sufficient in treatment of urinary bladder hypertrophy following partial bladder outlet obstruction PMID: 29572181
    17. characterized the phosphoproteome that is regulated by insulin as well as its key downstream kinases including Akt, mTORC1, and S6K. PMID: 28689409
    18. Deferoxamine increased P-AKT, HIF-1alpha and CXCR4 expression in mesenchymal stem cells compared to non-treated cells. PMID: 29341316
    19. Suggest that when cardiomyocytes incur oxidative stress under diabetic conditions, it subsequently activates the PI3Kgamma/Akt cell-signaling pathway and further increases HMGB1 expression. PMID: 27821807
    20. protein expression of Cyclin D1, p-Akt and Akt in glioma C6 cells decreased after transfection with miR-17 mimics for 72 h, and increased after transfection with miR-17 inhibitor for 72 h PMID: 29351283
    21. Taken together, our results demonstrate that HPC appears to confer neuroprotection against retinal IRI via the PI3K/Akt pathway. PMID: 29288664
    22. Taken together, our results demonstrated that reduced serum leptin, at least in part, contributes to exercise-mediated improvement of insulin sensitivity, indicating JAK2 as a potent therapeutical target of insulin resistance. PMID: 29294325
    23. Results from the present study indicated that pioglitazone may improve insulin sensitivity and ameliorate Abeta42 accumulation in rats with dietinduced IR by regulating AKT/GSK3beta activation, suggesting that pioglitazone may be a promising drug for AD treatment. PMID: 28447730
    24. AKT regulates post-synaptic expression of AMPA receptors affecting solely expression of early phase long-term potentiation (eLTP), with no effect on its induction and maintenance. Both maintenance of basal synaptic activity and expression of eLTP require plasma membrane tethering by activated AKT and that basal synaptic activity may be regulated via the direct effects of AKT1 on expression level of post-synaptic AMPARs. PMID: 27068236
    25. Taken together, we provide evidence that UC-MSCs therapy can successfully alleviate IC in a preclinical animal Model and cell model by alleviating inflammation, promoting proliferation and inhibiting apoptosis. PMID: 29137981
    26. results revealed that NaNO2 significantly increased serum tumor necrosis factor-alpha, C-reactive protein, heat shock proteins-70, vascular endothelial growth factor, and Interleukin 6. Moreover, transforming growth factor-beta, hypoxia-inducible factor, Smad-2, Protein Kinase B (AKT), and Bax were overexpressed, whereas Bcl2 protein was downregulated compared with the normoxic group. PMID: 28266762
    27. the Akt/HK2 pathway is likely to play a role in the development of a cardioprotective phenotype of CNH by preventing the detachment of HK2 from mitochondria at reperfusion period and decreases the Bax/Bcl-2 ratio during I/R insult, thereby lowering the probability of apoptosis activation in the mitochondrial compartment. PMID: 28290047
    28. These findings suggested that exercise dampened the secretion of inflammation mediators probably through partial inhibition of TLR4 and p-NF-kappaB and activation of PI3K/p-Akt expression in the spleen. PMID: 27539497
    29. hypoxia significantly decreased hemoglobin concentration, while increased expressions of HIF, Bax, Smad-2, TGF-beta, and AKT. PMID: 28000380
    30. in vitro assays confirmed upregulation of PIK3R2 upon knockdown of miR-126-3p in brain microvascular endothelial cells (BMECs), and silencing of miR-126-3p resulted in impaired BMEC barrier permeability and reversed vascular endothelial growth factor- and angiopoietin-1-induced activation of Akt and inhibition of BMEC apoptosis PMID: 29042193
    31. Data suggest that up-regulation of Akt1 phosphorylation and transport to nucleus drives osteoblast differentiation; here, oligopeptide from buffalo casein up-regulates Akt1 phosphorylation and transport to nucleus, gene expression, mineral deposition/calcification, and cell differentiation in cultured osteoblasts. These studies were conducted in India. PMID: 27736733
    32. Data suggest that insulin resistance of myotubes can be modulated by dietary factors; here, zinc, a dietary component and common dietary supplement, up-regulates glucose transport, Glut4 translocation, Akt phosphorylation, and Gsk3b phosphorylation/activation, and down-regulates mTOR and S6k1 in L6 cells. (Glut4 = glucose transporter 4; Akt = AKT serine/threonine kinase 1; Gsk3b = glycogen synthase kinase 3 beta) PMID: 27295130
    33. The Akt and NF-kappaB pathways are important for regulation of the early endothelial differentiation and its migratory ability under a hypoxic microenvironment. PMID: 28173835
    34. Here, the s discovered that, in the developing neuron from rat, Akt signaling regulates axon growth and growth cone formation through phosphorylation of serine 14 (S14) on Inhibitor of DNA binding 2 (Id2). PMID: 27938661
    35. Exendin-4 ameliorates HG-induced cardiomyocyte apoptosis, and the mechanisms may involve anti-oxidative stress via the HO-1/Nrf-2 system, as well as intervention of the PI3K/AKT signaling pathway. PMID: 28464602
    36. central injection of M617 mitigated insulin resistance of skeletal muscle by enhancing GLUT4 translocation from intracellular pools to plasma membranes via the activation of the Akt/AS160/GLUT4 signaling pathway. PMID: 27041232
    37. Results suggest that cilostazol could protect hippocampal neurons and ameliorate the impairment of learning/memory abilities and locomotor/exploratory activities in ischemic stroke via a PI3K-Akt1/JNK3/caspase-3 dependent mechanism PMID: 27769787
    38. Data suggest that ETA receptor antagonism is a mediator to attenuate sympathetic hyperinnervation probably through restoration of PI3K/Akt/GSK-3beta/ROS signaling pathway, a potential pharmacological target for arrhythmias after infarction. PMID: 27991911
    39. This study demonstrates that one of the critical mechanisms underlying curcumin inhibiting heat-induced apoptosis is through suppressing NADPH Oxidase 2 and activating the Akt/mTOR signaling pathway in bronchial epithelial cells. PMID: 28478460
    40. Findings indicate the importance of the long non-coding RNAs (lncRNAs)-XIST/miR-494/PTEN/AKT signaling axis in the pathogenesis of spinal cord injury (SCI) and suggest that XIST may be a promising molecular target for SCI therapy. PMID: 28368292
    41. In summary, our results suggest that HO-1 can decrease H/R-induced myocardiac cell apoptosis; the mechanism may be related to the activation of the Akt signaling pathway and, furthermore, to the inhibition of the JNK/c-Jun/caspase-3 signaling pathway. PMID: 27220977
    42. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
    43. Finally, we detected the variance during regeneration progress through the rat walk footprint test. In summary, all these evidences demonstrated that Sam68 might participate in Schwann cell proliferation partially via PI3K/Akt pathway and also regulate regeneration after sciatic nerve crush. PMID: 27059137
    44. novel evidence that exogenous H2S protects H9c2 cells against high glucose-induced injury by activating the HSP90/Akt pathway. PMID: 28204829
    45. study shows that SIRT2 regulates nuclear Nrf2 levels by modulating Akt phosphorylation, thus modulating the levels of GCL and total glutathione. PMID: 27264719
    46. the present study identified 14 differentially expressed kinase genes in human bone marrow endothelial cells in liver fibrosis (LF) from the microarray data, including p38a, AKT1 and PDK1.the results from the present study indicate that the p38a kinase inhibitor, SB203580, may exhibit a protective effect on bone marrow tissues in rats with LF PMID: 27748901
    47. trimetazidine exerts protective effects against cardiac I/R injury through cardiac miRNA21 expression, Akt, and the Bcl2/Bax pathway. Therefore, the present study provided evidence regarding the protective effects of miRNA21 on cardiac I/R injury following treatment with trimetazidine in vivo PMID: 27666568
    48. These results indicate that the anti-inflammatory effect of 6,6'-bieckol on LPS-stimulated microglial cells is mainly regulated by the inhibition of IkappaB-alpha/NF-kappaB and JNK/p38 MAPK/Akt pathways PMID: 27121731
    49. The effects of RNA interference-mediated GLT1 gene silencing on cell viability, JNK activation, NMDAR (NR1 and NR2B) activation, cell apoptosis and Akt activation in the hippocampal neuronal cells were slightly reversed by propofol treatment. PMID: 27430327
    50. results indicate that mechanical stress-regulated osteogenesis and adipogenesis of rat BMSCs are mediated, at least in part, by the PI3K/Akt/GSK-3beta/beta-catenin signaling pathway. PMID: 28286769

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
  • 组织特异性：
    Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
  • 数据库链接：

    KEGG: rno:24185

    STRING: 10116.ENSRNOP00000038369

    UniGene: Rn.11422