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  4. Recombinant Rat RAC-beta serine/threonine-protein kinase (Akt2)

Recombinant Rat RAC-beta serine/threonine-protein kinase (Akt2)

  • 货号：
    CSB-YP001555RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001555RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001555RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001555RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001555RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Akt2RAC-beta serine/threonine-protein kinase; EC 2.7.11.1; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-481
  • 氨基酸序列
    MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP PLNNFSVAEC QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWI RAIQMVANSL KQRGPGEDAM DYKCGSPSDS STSEMMEVAV SKARAKVTMN DFDYLKLLGK GTFGKVILVR EKATGRYYAM KILRKEVIIA KDEVAHTVTE SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGDLFFH LSRERVFTED RARFYGAEIV SALEYLHSTD VVYRDIKLEN LMLDKDGHIK ITDFGLSKEG ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHERLFE LILMEEIRFP RTLGPEAKSL LAGLLKKDPK QRLGGGPSDA KEVMEHRFFL SINWQDVVQK KLLPPFKPQV TSEVDTRYFD DEFTAQSITI TPPDRYDSLG SLELDQRTHF PQFSYSASIR E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the ...显示更多
  • 基因功能参考文献：
    1. Suggest that when cardiomyocytes incur oxidative stress under diabetic conditions, it subsequently activates the PI3Kgamma/Akt cell-signaling pathway and further increases HMGB1 expression. PMID: 27821807
    2. The results of this study suggest that phosphorylation of Akt2 mediates the beneficial effects of galanin on insulin-induced glucose uptake in the adipocytes of diabetic rats. PMID: 28365702
    3. Data suggest that caloric restriction in aging rats up-regulates insulin-stimulated glucose uptake in the predominantly slow-twitch soleus muscle; this up-regulation appears to rely on enhanced Akt2 phosphorylation. PMID: 27786542
    4. Evidence links greater Akt2 activation to the caloric restriction-induced elevation of insulin-stimulated glucose uptake by muscle from old animals. PMID: 26739650
    5. Nuclear translocation of Akt2 and synthesis of GLUT2 is increased in the hepatocytes of chard or/and insulin-treated hyperglycemic rats. PMID: 23746671
    6. PP2A inhibitors increased hepatic Akt phosphorylation and inhibited FoxO1in vitro and in vivo, and suppressed gluconeogenesis in hepatocytes PMID: 24150286
    7. In adult recuperated rats, Akt phosphorylation (P<0.01) and protein levels of Akt-2 (P<0.01) were also reduced. Messenger RNA expression levels of these proteins were not different, indicating a post-transcriptional effect. PMID: 23229735
    8. Hydrogen sulfide effects on cardiac relaxation involve KATP channels, the Akt/NOS-cGMP/PKG pathway, and S-sulfhydration of cardiac proteins. PMID: 23785074
    9. Akt2 mediates insulin-dependent Rac1 activation in muscle cells. PMID: 23499910
    10. In corpus striatum slices, basal activity of Akt2 regulates DAT cell surface expression. PMID: 22778840
    11. BAFF/BAFF-R involved in autoantibody production in rats with collagen-induced arthritis via PI3K-Akt-mTOR signaling and the regulation of paeoniflorin. PMID: 22391142
    12. analysis of resistance of Akt kinase and PP2A to dephosphorylation through ATP-dependent conformational plasticity PMID: 22031698
    13. Findings link prenatal hypoxia to down-regulation of components of hepatic and muscle Akt expression in adult offspring. PMID: 20923964
    14. Reduced Akt2 leads to up-regulation of Rab4a expression in cardiomyocytes in a cell-autonomous fashion that may involve activation of PPARalpha. PMID: 20728450
    15. The results suggest the possibility that an age-related decline in pAkt2Thr308, acting by a mechanism other than reduced pAS160Thr642, may play a role in the insulin resistance in the soleus of old rats. PMID: 20339009
    16. The downstream mediator of PI3-K, Akt/protein kinase B, also exhibited differentiation-dependent activation, which regulates the development of the differentiated trophoblast giant cell phenotype. PMID: 12089343
    17. Phosphorylation of cardiac protein kinase B is regulated by palmitate. PMID: 12181135
    18. These results suggest that, with brief calorie restriction, enhanced Akt2 phosphorylation may play a role in increasing insulin sensitivity in rat skeletal muscles. PMID: 12799317
    19. Akt inhibits BID-mediated apoptosis downstream of BID cleavage via promotion of mitochondrial hexokinase association and antagonism of tBID-mediated BAX and BAK activation at the mitochondria. PMID: 14701745
    20. reductions in expression of IRS-1 and insulin-stimulated phosphorylation of IRS-1 and Akt2 are associated with insulin resistance in mtDNA-depleted L6 GLUT4myc myocytes PMID: 15764607
    21. in skeletal muscle, insulin stimulates translocation of aPKC zeta and lambda, but not Akt2, to the plasma membrane PMID: 15802290
    22. The data reveal a novel type of cross-talk between P2Y12 and IGF-I receptors that proceeds through Gbetagamma-, Ca2+-and PLD2-dependent activation of the Pyk2/Src pathway resulting in GTP-loading of Rap1 required for an increased PKB phosphorylation. PMID: 16236484
    23. p122RhoGAP Ser-322 acts as an integrator of signal transduction in a manner dependent on the cellular context and is a substrate for protein kinase B and ribosomal S6 kinase PMID: 16338927
    24. kinetic analysis of Akt1, Akt2 and Akt3 shows that ATP is an initiating factor for the catalysis of AKT enzymes and may play a role in the regulation AKT enzyme activity in cells PMID: 16540465
    25. Akt-1 and Akt-2 have distinct roles in muscle glucose metabolism; moderate reductions in IRS-1 expression do not result in the development of insulin resistance in skeletal muscle in vivo PMID: 17021050
    26. EGF but not insulin specifically upregulated Akt 2 and 3. PMID: 18535289
    27. Endogenous protein kinase B-beta/Akt2 phosphorylates and inhibits the cardiac Na+/H+ exchanger NHE1. PMID: 18757828
    28. the anti-apoptotic properties of insulin are mediated partly by increasing HO-1 expression at transcriptional level via IRS1/PI3K/Akt2 activation PMID: 18854316
    29. Akt2, but not other isoforms, specifically regulates B23 sumoylation and protein stability PMID: 18931307
    30. results suggest a crosstalk between Akt2 and TGFbeta signaling that involves a mechanism requiring EGFR down-regulation during the entire tumor promotion process starting from the early stage PMID: 19309364
    31. PKB/Akt directly phosphorylates NR2C on serine 1096 PMID: 19477150

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Early endosome.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
  • 数据库链接：

    KEGG: rno:25233

    STRING: 10116.ENSRNOP00000025303

    UniGene: Rn.87066