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  4. Recombinant Rat Rho GDP-dissociation inhibitor 1 (Arhgdia)

Recombinant Rat Rho GDP-dissociation inhibitor 1 (Arhgdia)

  • 货号：
    CSB-YP736450RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP736450RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP736450RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP736450RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP736450RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ArhgdiaRho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-204
  • 氨基酸序列
    AEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK YKEALLGRVA VSADPNVPNV IVTRLTLVCS TAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PMEEAPKGML ARGSYNIKSR FTDDDKTDHL SWEWNLTIKK EWKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
  • 基因功能参考文献：
    1. Results show that RhoGDIalpha is transcriptionally regulated by ATF4 in neurons and that RhoGDIalpha knockown reduces Cdc42 expression supporting the view that RhoGDIalpha mediates the effects of ATF4 on Cdc42 and therefore on mushroom spines and excitatory synapses. PMID: 27841340
    2. The siRNA-mediated suppression of expression of alphaGDI especially affected vesicle dynamics. PMID: 26971292
    3. The regulatory functions of RhoGDIalpha were investigated by siRNA knockdown of RhoGDIalpha expression. After silencing RhoGDIalpha, the neuroprotective effects of senegenin on H/R-induced PC12 cell apoptosis were absent. PMID: 25367882
    4. RhoGDalpha controls ENaC expression and activity via Rac1. PMID: 25164814
    5. Mycophenolic acid induced apoptosis of transplanted islet cells via RhoGDI-alpha/Rac1/JNK pathway. PMID: 22564631
    6. A possible preemptive cross-talk between arginine methylation and serine and tyrosine phosphorylation was observed for Rho guanidine nucleotide dissociation inhibitor 1 (RhoGDI-1)in fibroblasts stimulated with epidermal growth factor. PMID: 17506542
    7. phosphorylated RhoA or phosphomimetic mutants trapped guanine dissociation inhibitor alpha (GDIalpha), leading to the release of Rac1 and its translocation to the membrane, where it was then activated by the basal Vav3 nucleotide exchange activity. PMID: 20696841
    8. results demonstrate that vascular remodeling results in significant alterations in the protein expression profile of the aorta during hypertension and suggest that the upregulation of RhoGDIalpha in hypertension is induced by Ang II via AT(1) receptor PMID: 17963997
    9. Low shear stress-induced vascular smooth muscle cell migration and apoptosis are mediated by a downregulation of Rho-GDIalpha. PMID: 18540023
    10. Data suggest that intermittent parathyroid hormone treatment induces changes in expression of many proteins in osteoblasts and results in RhoGDIalpha up-regulation both in vitro and in vivo when estrogen is present. PMID: 19575003

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Rho GDI family
  • 数据库链接：

    KEGG: rno:360678

    STRING: 10116.ENSRNOP00000051840

    UniGene: Rn.1801