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  4. Recombinant Saccharomyces cerevisiae Autophagy-related protein 14 (ATG14)

Recombinant Saccharomyces cerevisiae Autophagy-related protein 14 (ATG14)

  • 货号：
    CSB-YP339941SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP339941SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP339941SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP339941SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP339941SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ATG14; APG14; CVT12; YBR128C; YBR1003Autophagy-related protein 14; Cytoplasm to vacuole targeting protein 12
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-344
  • 氨基酸序列
    MHCPICHHRA HVVYCAHCIN TSPSLLLKLK LDLILLKDEN KELNGKVEQI LNEAMNYDQL DIKRMEKKKD PLMNSLMKLD VLRMKKNNNL IRHRIEQLNE RIYSKRNHIS ELKVEIDNYK CYKVGTGTDK LREQVEISDA KNKLAQVSKI CESARDYKLN LLNNWFVIQK LQDNFQIPFA IAFQPLISLK NFRILPLAIT NDSINIMWKY ISFFSDILMI KLPYTNKICE QPMFEFSDSI QTVVQRLIKL IINILQICRH LKLVPSTPMD IPWLLDQYDV DGLFYNMVKR NKMKCRSVSL YWTFGMLYSM VLDNMNNPQR GHPARRTAPP PTVTGPHDRW YVVG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase complex I to the preautophagosomal structure (PAS).
  • 基因功能参考文献：
    1. Deletion analysis of Atg14p revealed that N-terminal region containing the coiled-coil structures was essential and sufficient for autophagy. PMID: 16421251
  • 亚细胞定位：
    Preautophagosomal structure membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Note=TRS85 is required for the recruitment of ATG14 to the PAS.
  • 蛋白家族：
    ATG14 family
  • 数据库链接：

    KEGG: sce:YBR128C

    STRING: 4932.YBR128C