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  4. HEK293T/Human SARM1 Stable Cell Line

HEK293T/Human SARM1 Stable Cell Line

  • 货号：
    CSB-SC750971HU
  • 规格：
    1 vial contains approximately 5x106 cells in 1 ml
  • 价格：
    ￥30000
  • 活性：
    • Untransfected HEK293T cells (green line) and transfected Human SARM1 HEK293T Stable cells (red line) were stained with anti-Flag antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
    • Western Blot
      Positive WB detected in: Lane1：20 µg HEK293T cells, Lane2：40 µg Human SARM1 HEK293T Stable cells, Lane3：20 µg Human SARM1 HEK293T Stable cells
      All lanes: Anti-Flag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 77.5 KDa
      Observed band size: 77 KDa
  • 其他：

产品详情

  • Uniprot No.：
    Q6SZW1
  • 生长类型：
    Adherent
  • 抗性：
    Puromycin
  • 培养基：
    DMEM + 10% FBS + 2μg/mL Puromycin
  • 别名：
    SARM1
  • 蛋白标签：
    C-terminal Flag-tagged
  • 保存缓冲液：
    35% FBS + 55% DMEM + 10% DMSO
  • 应用范围：
    Binding assay by FACS and Western blot
  • 储存条件：
    Frozen in liquid nitrogen or stored at -80°C
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

靶点详情

  • 功能：
    NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
  • 基因功能参考文献：
    1. we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644
    2. Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829
    3. Data show that sterile alpha- and armadillo-motif-containing protein (SARM) modulates MyD88 protein-mediated Toll-like receptors (TLRs) activation through BB-loop dependent interleukin-1 receptor (TIR) TIR-TIR interactions. PMID: 26592460
    4. These results indicate that association of PINK1 with SARM1 and TRAF6 is an important step for mitophagy. PMID: 23885119
    5. The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling. PMID: 23923041
    6. Rapid Wallerian degeneration requires the pro-degenerative molecules SARM1. PMID: 24840802
    7. Data found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis. PMID: 24021647
    8. SARM overexpression caused mitochondrial clustering which has also been observed in several cell death phenomenon. PMID: 23175186
    9. The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856
    10. SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472
    11. Candidate gene in the onset of hereditary infectious/inflammatory diseases. PMID: 15893701
    12. TIR adaptor SARM is a negative regulator of Toll-like receptor signaling. PMID: 16964262
    13. confirmed the co-localization of retinoschisin with Na/K ATPase and SARM1 in photoreceptors and bipolar cells of retina tissue PMID: 17804407
    14. SARM1 deficiencies may uncover unexpected similarities between the ways in which neurons and immune cells sense and respond to danger. PMID: 18089857

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  • 亚细胞定位：
    Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
  • 组织特异性：
    Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta.
  • 数据库链接：

    HGNC: 17074

    OMIM: 607732

    KEGG: hsa:23098

    STRING: 9606.ENSP00000406738

    UniGene: Hs.743510