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  4. HA-Tag Monoclonal Antibody

HA-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000141M0m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: 3 different overexpression lysates with HA tagged
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35, 35, 48 kDa
      Observed band size: 35, 35, 48 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein
      All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
      All lanes: HA-Tag antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution

    • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

    • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

    • Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 其他：

产品详情

  • 产品描述：

    The product CSB-MA000141M0m is an unconjugated monoclonal antibody against HA-Tag. It is derived from the mouse myeloma cell-splenocyte hybridoma. The splenocyte is screened from the mouse immunized with the HA-Tag synthetic peptide conjugated to KLH and can secret HA-Tag antibody. This HA-Tag antibody can react with HA-Tag protein from all species. It is matched with the mouse IgG2b isotype. Protein A-mediated purification of this HA-Tag antibody makes its purity up to more than 95%. This antibody has been validated for use in ELISA, WB, IP, IF, and FC applications.

    The HA tag is a 9-amino acid peptide derived from the human influenza virus hemagglutinin (HA). It is widely used as a common epitope tag in expression vectors. Because the HA tag does not interfere with the bioactivity or biodistribution of the recombinant protein, it is often fused to the N-terminal or C-terminal of recombinant protein thus facilitating the detection, isolation, and purification of proteins of interest.

  • 别名：
    HA-Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    YPYDVPDYA synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    18B11H6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:5000-1:160000
    IF 1:50-1:200
    IP 1µg-5µg
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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