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  4. Recombinant Severe acute respiratory syndrome coronavirus Envelope small membrane protein (E)

Recombinant Severe acute respiratory syndrome coronavirus Envelope small membrane protein (E)

  • 货号：
    CSB-CF348676HQE
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    E
  • Uniprot No.：
  • 别名：
    E; sM; 4; Envelope small membrane protein; E protein; sM protein
  • 种属：
    Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-76
  • 氨基酸序列
    MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPTVYVYS RVKNLNSSEGVPDLLV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.
  • 基因功能参考文献：
    1. A structural model of a SARS-CoV E construct in LMPG micelles shows unequivocal intermolecular nuclear Overhauser effects. The C-terminal domain is mostly alpha-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation. PMID: 29474890
    2. distinct oligomeric forms of IBV E, one essential for assembly and the other with a role in disruption of the secretory pathway. PMID: 26136577
    3. Data show that the E protein has only one transmembrane (TM) domain in micelles, whereas the predicted beta-coil-beta motif forms a short membrane-bound alpha-helix connected by a disordered loop to the TM domain. PMID: 24668816
    4. These results suggested that the lipids are functionally involved in E protein ion channel activity, forming a protein-lipid pore. PMID: 22832120
    5. We showed that the SARS-CoV E protein is targeted to the Golgi region in HeLa cells and colocalizes most closely with GM130, which is a cis-Golgi protein. PMID: 21450821
    6. Data suggest that hijacking of PALS1 by SARS-CoV E plays a determinant role in the disruption of the lung epithelium in SARS patients. PMID: 20861307
    7. results show that the envelope (E) protein of severe acute respiratory syndrome-associated coronavirus can induce membrane permeability changes when expressed in Escherichia coli PMID: 15522242
    8. The E protein is not glycosylated. PMID: 15831954
    9. A novel molecular mechanism of T-cell apoptosis that contributes to the SARS-CoV-induced lymphopenia observed in most SARS patients is reported. PMID: 16048439
    10. expression of the SARS-CoV E protein in mammalian cells alters the membrane permeability of these cells; Systematic mutagenesis studies confirm that the membrane permeabilizing activity of the E protein is associated with its transmembrane domain PMID: 16507314
    11. The N- and C-termini of the SARS-CoV E protein are exposed to the cytoplasmic side of the membranes (N(cyto)C(cyto)). PMID: 16684538
    12. the expression of the structural protein, envelope (E), was down-regulated by 8b but not 8a or 8ab. PMID: 16876844
    13. analysis of the viroporin activity of SARS-CoV E protein PMID: 17037530
    14. conformational flexibility required during ion channel function PMID: 18658207
    15. both E and N proteins must be coexpressed with M protein for the efficient production and release of virus-like particles by transfected Vero E6 cells. PMID: 18753196
    16. Both the transmembrane domains of E protein are required to interact with M protein, while either of the hydrophilic regions (a.a. 1-10 or 60-76) is dispensable as shown by co-immunoprecipitation assay. PMID: 19322648

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  • 亚细胞定位：
    Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus membrane; Single-pass type III membrane protein.
  • 蛋白家族：
    Betacoronaviruses E protein family
  • 数据库链接：

    KEGG: vg:1489671