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Recombinant Mouse Sialidase-4 (Neu4)

  • 货号：
    CSB-CF803970MO
  • 规格：
    ￥5268
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Neu4
  • Uniprot No.：
  • 别名：
    Neu4; Sialidase-4; N-acetyl-alpha-neuraminidase 4; Neuraminidase 4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 表达区域：
    1-478aa
  • 氨基酸序列
    MGPTRVPRRTVLFQRERTGLTYRVPALLCVPPRPTLLAFAEQRLSPDDSHAHRLVLRRGTLTRGSVRWGTLSVLETAVLEEHRSMNPCPVLDEHSGTIFLFFIAVLGHTPEAVQIATGKNAARLCCVTSCDAGLTWGSVRDLTEEAIGAALQDWATFAVGPGHGVQLRSGRLLVPAYTYHVDRRECFGKICWTSPHSLAFYSDDHGISWHCGGLVPNLRSGECQLAAVDGDFLYCNARSPLGNRVQALSADEGTSFLPGELVPTLAETARGCQGSIVGFLAPPSIEPQDDRWTGSPRNTPHSPCFNLRVQESSGEGARGLLERWMPRLPLCYPQSRSPENHGLEPGSDGDKTSWTPECPMSSDSMLQSPTWLLYSHPAGRRARLHMGIYLSRSPLDPHSWTEPWVIYEGPSGYSDLAFLGPMPGASLVFACLFESGTRTSYEDISFCLFSLADVLENVPTGLEMLSLRDKAQGHCWPS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    制备重组小鼠唾液酸酶-4 (Neu4)的一般步骤：
    获取并克隆Neu4基因：从小鼠组织中提取总RNA，利用逆转录酶和PCR扩增技术获得Neu4基因。将扩增后的Neu4基因插入到表达载体中，如pET系列载体中。
    转染宿主细胞：将含有Neu4基因的表达载体转染到宿主细胞中。可以选择常见的哺乳动物细胞系，如CHO细胞、HEK293细胞等。
    表达Neu4蛋白：根据表达载体中的启动子和调节元件，诱导Neu4蛋白在转染的宿主细胞中表达。在培养期间，需要添加抗生素等来保持表达载体的稳定。
    收集细胞裂解液：利用细胞破碎和离心等方法，收集含有Neu4蛋白的细胞裂解液。
    纯化Neu4蛋白：采用亲和层析、离子交换层析、凝胶过滤层析等方法对Neu4蛋白进行纯化。
    鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的Neu4蛋白进行鉴定和定量。此外，可以使用唾液酸酶活性分析等方法来检测其生物学活性和功能。
    需要注意的是，唾液酸酶是一种糖酶，需要在适当的pH和温度下进行表达和纯化。此外，唾液酸酶在储存和使用时容易受到蛋白质降解酶的影响，因此需要注意保存条件。
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons. May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation. Has sialidase activity toward mucin, fetuin and sialyllactose.
  • 基因功能参考文献：
    1. These results provide the first in vivo evidence that neuraminidases 3 and 4 have important roles in CNS function by catabolizing gangliosides and preventing their storage in lipofuscin bodies. PMID: 28442549
    2. NEU4 is involved in regulation of neuronal function by polySia degradation in mammals. PMID: 22393058
    3. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice. PMID: 20862357
    4. Neu4 is a functional component of the ganglioside-metabolizing system, contributing to the postnatal development of the brain and other vital organs. PMID: 18270209
    5. mouse Neu4 plays an important regulatory role in neurite formation, possibly through desialylation of glycoproteins PMID: 19506080

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Cell projection, neuron projection. Lysosome lumen.
  • 蛋白家族：
    Glycosyl hydrolase 33 family
  • 组织特异性：
    Highly expressed in brain, particularly in hippocampus, and at lower levels in liver and spleen. Expressed in hippocampal neurons (at protein level).
  • 数据库链接：

    KEGG: mmu:241159

    STRING: 10090.ENSMUSP00000051151

    UniGene: Mm.214565