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  4. Human Lipopolysaccharides,LPS ELISA Kit

Human Lipopolysaccharides,LPS ELISA Kit

  • 中文名称：
    人脂多糖/内毒素(LPS)酶联免疫试剂盒
  • 货号：
    CSB-E09945h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        LPS (Lipopolysaccharide) 是一种内毒素，存在于许多革兰阴性菌的细胞外膜中，它的存在对于细菌的生长和生存是必要的。LPS的主要作用是激活免疫系统，引起免疫细胞的炎症反应，同时也可以引发严重的炎症反应和休克。在体内，LPS可以通过血液-脑屏障和血液-脊髓液屏障进入中枢神经系统，引起神经炎症反应和神经系统损伤。
        j9九游会登录入口首页生物所提供的Human Lipopolysaccharides,LPS ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的LPS，其灵敏度为1.56 pg/mL，检测范围为6.25 pg/mL-400 pg/mL。
     
  • 缩写：
    LPS
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-94  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 92  
    Range % 87-96  
    1:8 Average % 94  
    Range % 89-99  
  • 回收率：
    The recovery of human LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-97  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    400 2.809 2.960 2.885 2.720  
    200 2.227 2.366 2.297 2.132  
    100 1.515 1.556 1.536 1.371  
    50 0.789 0.755 0.772 0.607  
    25 0.432 0.470 0.451 0.286  
    12.5 0.303 0.325 0.314 0.149  
    6.25 0.235 0.232 0.234 0.069  
    0 0.164 0.165 0.165 　  
  • 本试剂盒所含材料：
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled LPS antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. HRP catalyzes TMB, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

为什么不同的物种有不同的ELISA试剂盒。是因为LPS的变化吗?我可以用人LPS ELISA检测小鼠血浆中的LPS吗?

A:
不建议跨物种检测。因为不同物种基质是不同的。

靶点详情

  • 最新研究进展：
    LPS是一种脂多糖，存在于细菌细胞壁中，可引发严重的炎症反应。最近的研究表明，LPS可以激活多种免疫细胞，如单核细胞和树突状细胞，并诱导它们释放多种炎症介质。此外，LPS也被认为是引发多种疾病的重要因素之一，例如感染、肠道炎症和自身免疫性疾病等。研究人员正在努力开发抑制LPS介导的免疫反应的新型治疗药物。