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  4. Recombinant Influenza A virus Nucleoprotein (NP)

Recombinant Influenza A virus Nucleoprotein (NP)

In Stock
  • 货号：
    CSB-EP302450IGF
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302450IGF could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Shanghai/16/1989 H3N2) NP.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP302450IGF could indicate that this peptide derived from E.coli-expressed Influenza A virus (strain A/Shanghai/16/1989 H3N2) NP.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NP
  • Uniprot No.：
  • 别名：
    NP; Nucleoprotein; Nucleocapsid protein; Protein N
  • 种属：
    Influenza A virus
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    60.2kDa
  • 表达区域：
    1-498aa
  • 氨基酸序列
    MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTVERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYKRVGGRWMRELVLYDKEEIRRIWRQANNGDDATRGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFSARSALILRGSVAHKSCLPACVYGPAVSSGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRLLSFIRGTKVSPRGKLSTRGVQIASNENMDNMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFEKSTVMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEVSFRGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEYDN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    A DNA sequence encoding the 1-498 aa of Influenza A virus was expressed with an N-terminal 6xHis-tag in E.coli. The resulting protein is a recombinant full-length Influenza A virus Nucleoprotein (NP). Its identity was confirmed by the LC-MS/MS Analysis. SDS-PAGE assay determined its purity greater than 90%. This NP protein may be used for specific antibody synthesis or in the studies of the microbiology research area.

    Influenza A virus NP is a structural protein with the most abundant expression and no intrinsic enzymatic activity in infected cells. It mainly encapsidates the segmented RNA and then combines with the three polymerase subunits (PA, PB1, and PB2) to form the viral ribonucleoprotein particles (vRNPs) for RNA transcription ＆ replication, and packaging. Various nuclear localization signals (NLS) in the amino acid sequence of NP are responsible for the active RNP import into the nucleus. Besides, NP is one of the main determinants of influenza species specificity. The self-oligomerization of NP is essential for its function.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Influenza viruses nucleoprotein family