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  4. Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

  • 中文名称：
    牛α2-HS糖蛋白(AHSG)酶联免疫试剂盒
  • 货号：
    CSB-EL001485BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine AHSG ELISA Kit was designed for the quantitative measurement of Bovine AHSG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 ng/mL-8000 ng/mL and the sensitivity is 31.25 ng/mL.

  • 别名：
    AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
  • 缩写：
    AHSG
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 ng/mL-8000 ng/mL
  • 灵敏度：
    31.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 92-98  
    1:200 Average % 97  
    Range % 93-102  
    1:400 Average % 102  
    Range % 98-106  
    1:800 Average % 88  
    Range % 82-96  
  • 回收率：
    The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 104 99-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.073 0.072 0.073    
    4000 0.138 0.136 0.137    
    2000 0.255 0.242 0.249    
    1000 0.435 0.447 0.441    
    500 0.675 0.686 0.681    
    250 1.038 1.049 1.044    
    125 1.347 1.248 1.298    
    0 1.593 1.695 1.644    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
  • 基因功能参考文献：
    1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
    2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
    3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Fetuin family
  • 组织特异性：
    Liver and bone.
  • 数据库链接：