Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Bovine β-OHB ELISA Kit was designed for the quantitative measurement of Bovine β-OHB protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 50 nmol/mL-800 nmol/mL and the sensitivity is 9.5 nmol/mL.
别名：

beta-Hydroxybutyric acid, beta-OHB
缩写：

β-OHB
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

50 nmol/mL-800 nmol/mL
灵敏度：

9.5 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	98
	Range %	92-104
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-103
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.