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中文名称:牛胱天蛋白酶7(Casp-7)酶联免疫试剂盒
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货号:CSB-E17315B
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Bovine Casp7 ELISA Kit was designed for the quantitative measurement of Bovine Casp7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
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缩写:
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Uniprot No.:
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种属:Bos taurus (Bovine)
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.625 ng/mL-40 ng/mL
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灵敏度:0.156 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cell Biology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 85-101 1:2 Average % 95 Range % 88-102 1:4 Average % 89 Range % 83-100 1:8 Average % 93 Range % 89-106 -
回收率:
The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 90-112 EDTA plasma (n=4) 93 84-99 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.546 2.457 2.502 2.369 20 1.883 1.905 1.894 1.761 10 1.136 1.124 1.130 0.997 5 0.726 0.711 0.719 0.586 2.5 0.407 0.397 0.402 0.269 1.25 0.305 0.313 0.309 0.176 0.625 0.196 0.204 0.200 0.067 0 0.130 0.136 0.133 -
数据处理:
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货期:3-5 working days
引用文献
- Seasonal variation in HSP70 expression and oxidative stress in skin of zebu (Tharparkar) and crossbred (Karan Fries) cattle under tropical climate Uttarani Maibam.et al,BIOL RHYTHM RES,2017
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