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  4. Bovine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

Bovine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

  • 中文名称：
    牛成纤维细胞生长因子23(FGF23)酶联免疫试剂盒
  • 货号：
    CSB-E16193B
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Bovine FGF23 ELISA Kit was designed for the quantitative measurement of Bovine FGF23 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 86-99    
    1:2 Average % 103    
    Range % 97-107    
    1:4 Average % 91    
    Range % 81-94    
    1:8 Average % 100    
    Range % 90-105    
  • 回收率：
    The recovery of bovine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 94-108    
    EDTA plasma (n=4) 89 84-94    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 1.928 1.952 1.940 1.809    
    500 1.402 1.324 1.363 1.232    
    250 0.878 0.862 0.870 0.739    
    125 0.553 0.540 0.547 0.416    
    62.5 0.329 0.341 0.335 0.204    
    31.2 0.241 0.256 0.249 0.118    
    15.6 0.178 0.174 0.176 0.045    
    0 0.129 0.133 0.131 　    
  • 数据处理：
  • 货期：
    3-5 working days

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