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  4. Bovine Fibronectin,FN ELISA Kit

Bovine Fibronectin,FN ELISA Kit

  • 中文名称：
    牛纤连蛋白(FN)酶联免疫试剂盒
  • 货号：
    CSB-E13781B
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Bovine FN1 ELISA Kit was designed for the quantitative measurement of Bovine FN1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.137 μg/mL-100 μg/mL and the sensitivity is 0.137 μg/mL.

  • 别名：
    FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    0.137 μg/mL-100 μg/mL
  • 灵敏度：
    0.137 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 92    
    Range % 85-102    
    1:10 Average % 97    
    Range % 91-110    
    1:20 Average % 89    
    Range % 83-96    
    1:40 Average % 95    
    Range % 84-100    
  • 回收率：
    The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 88-105    
    EDTA plasma (n=4) 97 92-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    µg/ml OD1 OD2 Average Corrected    
    100 1.839 1.803 1.821 1.677    
    33 1.657 1.649 1.653 1.509    
    11 1.451 1.435 1.443 1.299    
    3.7 0.958 0.992 0.975 0.831    
    1.2 0.643 0.667 0.655 0.511    
    0.4 0.372 0.354 0.363 0.219    
    0.13 0.251 0.243 0.247 0.103    
    0 0.145 0.142 0.144 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
  • 数据库链接：