Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Bovine Haptoglobin,Hpt/HP ELISA KIT

Bovine Haptoglobin,Hpt/HP ELISA KIT

  • 中文名称：
    牛结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒
  • 货号：
    CSB-E08585b
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    The bovine haptoglobin (HP) ELISA Kit is engineered for accurate measurement of bovine haptoglobin levels from samples including serum or plasma. It uses the cmpetitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the bovine haptoglobin content in the sample. The color intensity is negatively correlated with haptoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

    Haptoglobin is an acute-phase protein that is synthesized mainly in the liver and lungs and is crucial for the elimination of free hemoglobin and the neutralization of oxidative damage. It has powerful regulatory activity on lymphocytes. Additionally, haptoglobin exhibits immunoregulatory properties, participates in angiogenesis and inhibition of nitric oxide, stimulates tissue repair, etc.

  • 别名：
    HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    7.81 ng/mL-2000 ng/mL
  • 灵敏度：
    7.81 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 84  
    Range % 80-88  
    1:2000 Average % 90  
    Range % 85-94  
    1:4000 Average % 103  
    Range % 99-112  
    1:8000 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.243 0.252 0.248    
    500 0.412 0.436 0.424    
    120 0.793 0.754 0.774    
    31.25 1.214 1.239 1.227    
    7.81 1.795 1.724 1.760    
    0 2.405 2.359 2.382    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
The antibody used in the kit is rabbit polyclonal antibody.
The standard is purified crude extract of native samples.

靶点详情

  • 功能：
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
  • 基因功能参考文献：
    1. These results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and Hp in the serum and uterine washings. PMID: 25704413
    2. These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins. PMID: 25358728
    3. An increase in serum amyloid A or haptoglobin in dairy cows with abomasal displacementis not specific for inflammation or bacterial infection; increased levels of either may indicate the presence of hepatic lipidosis. PMID: 20391639
    4. Haptoglobin concentration may be a useful indicator for cows with postpartum reproductive disorders. PMID: 14960809
    5. Presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A to see the relationships in cow composite and bulk tank milk samples. PMID: 17227596
    6. Bovine granulocytes, isolated from peripheral blood of healthy cattle, contain abundant amounts of haptoglobin within the granules. PMID: 17681384
    7. This study suggests that neutrophils and epithelial cells may play an essential role in elevating milk haptoglobin (Hp) in addition to previous suggestions that Hp may be derived from mammary tissues and circulation. PMID: 19094922

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：