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  4. Bovine Immunoglobulin A,IgA ELISA Kit

Bovine Immunoglobulin A,IgA ELISA Kit

  • 中文名称：
    牛免疫球蛋白A(IgA)酶联免疫试剂盒
  • 货号：
    CSB-E12018B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine IgA ELISA Kit was designed for the quantitative measurement of Bovine IgA protein in serum, plasma, cell culture supernates, tissue homogenates_x005f_x000D_ _x005f_x000D_ . It is a Competitive ELISA kit, its detection range is 0.39 μg/mL-25 μg/mL and the sensitivity is 0.39 μg/mL.

  • 缩写：
    IgA
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.39 μg/mL-25 μg/mL
  • 灵敏度：
    0.39 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-99  
    1:200 Average % 96  
    Range % 92-101  
    1:400 Average % 85  
    Range % 81-87  
    1:800 Average % 89  
    Range % 83-94  
  • 回收率：
    The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-95  
    EDTA plasma (n=4) 97 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    25 0.199 0.187 0.193    
    12.5 0.325 0.329 0.327    
    6.25 0.466 0.489 0.478    
    3.12 0.731 0.760 0.746    
    1.56 1.017 1.105 1.061    
    0.78 1.310 1.410 1.360    
    0.39 1.526 1.570 1.548    
    0 2.125 2.162 2.144    
  • 数据处理：
  • 货期：
    3-5 working days

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