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  4. Bovine Interferon α,IFN-α ELISA Kit

Bovine Interferon α,IFN-α ELISA Kit

  • 中文名称：
    牛α干扰素(IFN-α)酶联免疫试剂盒
  • 货号：
    CSB-E13726B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine IFN-α ELISA Kit was designed for the quantitative measurement of Bovine IFN-α protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.

  • 别名：
    Interferon alpha-1 ELISA Kit
  • 缩写：
    IFN-α
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    25 pg/mL-1000 pg/mL
  • 灵敏度：
    25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-101  
    1:2 Average % 91  
    Range % 85-98  
    1:4 Average % 95  
    Range % 90-103  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of bovine IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 97 91-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.156 0.149 0.153    
    500 0.362 0.336 0.349    
    230 0.561 0.541 0.551    
    75 0.865 0.856 0.861    
    25 1.542 1.508 1.525    
    0 2.390 2.356 2.373    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：