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  4. Bovine Interferon β,IFN-β/IFNB ELISA Kit

Bovine Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名称：
    牛β干扰素(IFN-β/IFNB)酶联免疫试剂盒
  • 货号：
    CSB-E13699B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 别名：
    IFNB1Interferon beta-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
  • 检测范围：
  • 灵敏度：
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFNB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 83-107  
    1:2 Average % 89  
    Range % 86-98  
    1:4 Average % 94  
    Range % 83-102  
    1:8 Average % 93  
    Range % 82-105  
  • 回收率：
    The recovery of bovine IFNB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 95 86-104  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.134 0.138 0.136    
    80 0.241 0.225 0.233    
    48 0.324 0.305 0.315    
    20 0.586 0.618 0.602    
    8 1.253 1.234 1.244    
    0 2.598 2.671 2.635    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Has antiviral, antibacterial and anticancer activities.
  • 基因功能参考文献：
    1. BVDV2-E significantly increased IFN-beta activity compared to BVDV2-wt. PMID: 28432927
    2. The data confirm the involvement of EHMT2 in the epigenetic regulation of IFN-b and demonstrate the activation of a general antiviral state after EHMT2 inhibition. PMID: 26418342
    3. The s provide evidence that ICP27 protein encoded by bovine herpesvirus type 1, a viral early protein that shuttles between the nucleus and cytoplasm inhibits transcriptional activity of two bovine IFN-beta gene promoters (IFN-beta1 and IFN-beta3). PMID: 22902481
    4. The s demonstrate that bovine herpesvirus 1 bICP0 effectively inhibits bovine IFN-beta promoter activity and induces IRF3 degradation. PMID: 21689696
    5. These studies provide evidence that virus infection differentially stimulates expression of the three bovine IFN-beta genes. PMID: 21316405
    6. recombinant bovine respiratory syncytial virus lacking the NS proteins, and those lacking NS2 in particular, are strong inducers of IFN-alpha/beta in bovine nasal fibroblasts and bronchoalveolar macrophages. PMID: 12857912

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：