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Bovine Myoglobin(MB) ELISA kit

  • 中文名称：
    牛肌红蛋白(MB)酶联免疫试剂盒
  • 货号：
    CSB-EL013529BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Bovine Myoglobin(MB) protein in serum, plasma, tissue Homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    MB ELISA Kit; Myoglobin ELISA Kit
  • 缩写：
    MB
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue Homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 83-99
    1:2 Average % 91
    Range % 89-99
    1:4 Average % 95
    Range % 90-100
    1:8 Average % 95
    Range % 86-98
  • 回收率：
    The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-99
    EDTA plasma (n=4) 95 90-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    1000 2.184 2.201 2.193 2.099
    500 1.628 1.643 1.636 1.542
    250 1.023 1.015 1.019 0.925
    125 0.565 0.575 0.570 0.476
    62.5 0.318 0.305 0.312 0.218
    31.2 0.179 0.192 0.186 0.092
    15.6 0.129 0.133 0.131 0.037
    0 0.095 0.092 0.094 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Do you have both Beef and buffalo Myoglobin (MB) detection kit in ELISA format.Could you please discuss with your technical team for the following : 1. Cross-reactivity of Bovine MB kit for Buffalo MB. I am awaiting your response.

A:
Hi
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We only test ordinary bovine.But there should be no big problems on Beef and buffalo.We suggest you do pre-testing first and see
Q:

Could you please revisit/discuss once more with your technical team for the following : 1. Coating Antibody is polyclonal - Yes or No. 2. Biotin Labeled antibody is polyclonal - Yes or No.
I am awaiting your response.

A:
Bovine Myoglobin(MB) ELISA kit,#CSB-EL013529BO This kit is specific to general bovine.We did not valiadate this kit on Buffalo.But there should be no big problems.We suggest you do pre-testing first and see

靶点详情

  • 功能：
    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
  • 基因功能参考文献：
    1. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions. PMID: 26946475
    2. Glycine at E14 in myoglobin enhances autoxidation and hemin loss rates. PMID: 22681513
  • 蛋白家族：
    Globin family
  • 数据库链接：