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Bovine Plasminogen(PLG) ELISA kit

  • 中文名称：
    牛纤维蛋白溶解酶原(PLG)酶联免疫试剂盒
  • 货号：
    CSB-EL018188BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        PLG (plasminogen)纤溶酶原（plasminogen，PLG）是一种血浆中的前酶，通过t-PA的作用转化为纤溶酶（plasmin），参与血液的纤维蛋白溶解过程。纤溶酶原在体内广泛存在，主要由肝脏合成，但也可以由其它细胞如内皮细胞、巨噬细胞等合成。在纤溶酶抑制剂（PAI）的存在下，纤溶酶原的活性可以被抑制，从而控制纤溶过程的平衡。纤溶酶原的水平可以反映纤溶系统的活性，其升高常见于许多疾病的发生发展中，如心脑血管疾病、癌症等。
        j9九游会登录入口首页生物所提供的Bovine Plasminogen(PLG) ELISA kit属于ELISA检测试剂盒，采用竞争法定量检测牛血清、血浆、组织匀浆样本中的PLG，其灵敏度为15.6 ng/mL，检测范围为62.5 ng/mL-4000 ng/mL。
     
  • 别名：
    PLG ELISA Kit; Plasminogen ELISA Kit; EC 3.4.21.7) [Cleaved into: Plasmin heavy chain A; Activation peptide; Plasmin heavy chain A ELISA Kit; short form; Plasmin light chain B] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 ng/mL-4000 ng/mL
  • 灵敏度：
    15.6 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 97    
    Range % 93-102    
    1:200 Average % 85    
    Range % 81-90    
    1:400 Average % 102    
    Range % 97-106    
    1:800 Average % 91    
    Range % 86-95    
  • 回收率：
    The recovery of bovine PLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 93 87-96    
    EDTA plasma (n=4) 105 98-109    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    5000 0.102 0.099 0.101      
    2500 0.167 0.160 0.164      
    1250 0.254 0.241 0.248      
    625 0.372 0.365 0.369      
    312.5 0.558 0.544 0.551      
    156.2 0.766 0.713 0.740      
    78 1.024 0.984 1.004      
    0 1.834 1.765 1.800      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    PLG是一种广泛存在于人体内的血浆蛋白，最近的研究表明PLG在多种疾病的发生和发展中可能发挥着重要的作用。
  • 功能：
    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
  • 基因功能参考文献：
    1. SUPA formed a higher avidity complex (118-fold) with bovine Pg than with human Pg and non-proteolytically activated bovine but not human Pg. PMID: 22518846
    2. The plasminogen/plasminogen activator/plasmin system is activated during gamete interaction and regulates the sperm entry into the oocyte. PMID: 22177313
    3. The current study suggests the existence of an active TNF-alpha-plasminogen-plasmin autocrine/paracrine loop on the massively infiltrated polymorphonuclear neutrophils inside udders of drying-off cows. PMID: 19638263
    4. examination of interaction with streptococcal plasminogen activator PauA PMID: 15351638
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasminogen subfamily
  • 数据库链接：

    KEGG: bta:280897

    STRING: 9913.ENSBTAP00000001674

    UniGene: Bt.306