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  4. Bovine Selenoprotein P(SEPP1) ELISA kit

Bovine Selenoprotein P(SEPP1) ELISA kit

  • 中文名称：
    牛硒蛋白P(SEPP1)酶联免疫试剂盒
  • 货号：
    CSB-EL021018BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine SEPP1 ELISA Kit was designed for the quantitative measurement of Bovine SEPP1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 ng/mL-160 ng/mL and the sensitivity is 5 ng/mL.

  • 别名：
    SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
  • 缩写：
    SEPP1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    10 ng/mL-160 ng/mL
  • 灵敏度：
    5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 89
    Range % 86-102
    1:200 Average % 97
    Range % 91-105
    1:400 Average % 93
    Range % 88-100
    1:800 Average % 91
    Range % 87-96
  • 回收率：
    The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 86-99
    EDTA plasma (n=4) 93 85-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    160 0.105 0.102 0.104 　
    80 0.196 0.189 0.193 　
    40 0.321 0.332 0.327 　
    20 0.622 0.608 0.615 　
    10 1.098 1.108 1.103 　
    0 2.193 2.216 2.205 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Selenoprotein P family
  • 组织特异性：
    Brain and kidney. Most prominently expressed in the cerebellar cortex, hippocampus and olfactory bulb.
  • 数据库链接：

    KEGG: bta:282066

    UniGene: Bt.64610