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  4. Bovine Serum amyloid A,SAA ELISA Kit

Bovine Serum amyloid A,SAA ELISA Kit

  • 中文名称：
    牛血清淀粉样蛋白A(SAA)酶联免疫试剂盒
  • 货号：
    CSB-E08592b
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine SAA1 ELISA Kit was designed for the quantitative measurement of Bovine SAA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.1 μg/mL-20 μg/mL and the sensitivity is 0.05 μg/mL.

  • 别名：
    SAA1 ELISA Kit; SAA ELISA Kit; Serum amyloid A protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
  • 缩写：
    SAA1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.1 μg/mL-20 μg/mL
  • 灵敏度：
    0.05 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 93  
    Range % 89-98  
    1:4 Average % 95  
    Range % 91-98  
    1:8 Average % 92  
    Range % 90-95  
  • 回收率：
    The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-99  
    EDTA plasma (n=4) 96 89-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    0 1.963 1.977 1.970    
    0.1 1.644 1.654 1.649    
    0.4 1.239 1.245 1.242    
    1.6 0.817 0.798 0.808    
    5 0.372 0.368 0.370    
    20 0.121 0.123 0.122    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 基因功能参考文献：
    1. polymorphisms in the SAA2 gene are associated with milk production traits in Chinese Holstein cows PMID: 26373797
  • 相关疾病：
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    SAA family
  • 组织特异性：
    Expressed by the liver; secreted in plasma.
  • 数据库链接：