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Bovine TNF-αELISA kit

  • 中文名称：
    牛肿瘤坏死因子α（TNF-α）酶联免疫试剂盒
  • 货号：
    CSB-E12020B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        TNF（肿瘤坏死因子，tumor necrosis factor）是一种细胞因子，主要由巨噬细胞、T淋巴细胞、自然杀伤细胞等产生。它具有多种生物学功能，包括细胞凋亡、炎症、免疫调节和细胞增殖等。TNF通过激活多种信号转导通路，介导宿主对细菌、病毒和其他致病微生物的免疫应答，同时也对肿瘤的发生、发展有重要作用。
        j9九游会登录入口首页生物所提供的Bovine TNF-αELISA kit属于ELISA检测试剂盒，采用竞争法定量检测牛血清、血浆、组织匀浆样本中的TNF，其灵敏度为0.05 ng/mL，检测范围为0.1 ng/mL-20 ng/mL。
     
  • 别名：
    TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.1 ng/mL-20 ng/mL
  • 灵敏度：
    0.05 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 88-101  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 96  
    Range % 90-100  
  • 回收率：
    The recovery of bovine TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-101  
    EDTA plasma (n=4) 95 89-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.173 0.176 0.175    
    5 0.312 0.325 0.319    
    1.6 0.617 0.634 0.626    
    0.4 1.035 1.118 1.077    
    0.1 1.396 1.405 1.401    
    0 2.513 2.546 2.530    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Serum
    Sample species: Bovine
    Review: The average TNF-alpha serum concentration observed was 0.43 ng/ml. Inter-plate CV of in house pool was 6.5%. All intra-plate CV\'s for individual samples were below 10%.

靶点详情

  • 最新研究进展：
    TNF是一种介导炎症反应和细胞凋亡的细胞因子。最新研究表明，TNF在多种疾病的发生和发展中扮演着重要角色，包括炎症性疾病、自身免疫疾病、感染和肿瘤等。近年来，一些新型TNF抑制剂已经应用于临床治疗，如重症肺结核和风湿性关节炎等，表现出了良好的疗效和安全性。此外，一些新型TNF受体结构域融合蛋白的研究也为开发更有效的TNF抑制剂提供了新思路。
  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能参考文献：
    1. Data suggest that luteolytic factors (such as TNFa, interferon gamma, and PGF2a) control expression of MMP1, other matrix metalloproteinases, and tissue inhibitors of metalloproteinase in cultured luteal cells. PMID: 25924700
    2. These results suggest that polymorphism of the TNFalpha-824 A>G gene and mTNFalpha protein expression play an important role in the pathogenesis of enzootic bovine leukosis. PMID: 26618585
    3. These results suggest that the endometrium might lower the TNF concentration in the blastocyst by (1) regulating TNF secretion into the uterine fluid and (2) inducing decreased TNF and TNFR2 mRNA transcription in the embryo. PMID: 25589228
    4. There was a significant association between the proviral load and a low frequency of the G/G genotype of TNF-alpha at position -824. PMID: 26051703
    5. SNP in the TNF-alpha gene affects immune function and reproductive performance in dairy cows. PMID: 24562592
    6. Studied genotypic and expression profiling of partial TNF-alpha gene and its association with mastitis susceptibility in 129 crossbred cattle. PMID: 25380461
    7. Messenger RNA and protein levels of prostaglandin (PG) E synthase (PGES), PGF2alpha receptor (PGFR), tumor necrosis factor-alpha (TNF) and Fas were found to be higher in the corpus luteum of pregnancy than in corpus luteum of the cycle. PMID: 24856470
    8. TNF-alpha up-regulates NaV1.7 mRNA in both adrenal chromaffin cells and dorsal root ganglia (DRG) neurons, highlighting the peripheral nociceptive mechanism of TNF-alpha PMID: 24445633
    9. These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFalpha and iNOS pathways in the regulation of this pathological condition. PMID: 24597304
    10. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
    11. the effects of lysophopatidic acid on TNFalpha and IFNgamma - induced decrease of progesterone synthesis and on the cytokine - induced apoptosis of the cultured luteal cells. PMID: 23856004
    12. potential DNA markers in the improvement of immunity to mastitis PMID: 23758855
    13. A role for TNFalpha in intervertebral disc degeneration: a non-recoverable catabolic shift. PMID: 23438440
    14. Results indicate that TNF-alpha does not affect autonomous, pulsatile progesterone (P(4)) release, increases P(4) secretion by bovine corpus luteum (CL) with increasing dose & reduces in a dose-dependent manner responsiveness of CL to luteotropic factors. PMID: 21256693
    15. The differences in genetic polymorphism of TNFalpha between dairy dairy cattle herds infected and not infected with the bovine leukemia virus are reported. PMID: 22439343
    16. These results suggest that TNF-alpha sources include immune cells, as well as large and small luteal cells, and that TNF-RI and TNF-RII are present in the luteal cells of the bovine corpus luteum. PMID: 21551345
    17. Role of TGF-beta1 and TNF-alpha in IL-1beta mediated activation of proMMP-9 in pulmonary artery smooth muscle cells: involvement of an aprotinin sensitive protease. PMID: 21722622
    18. Plasma tumor necrosis factor-alpha response to either of two lipopolysaccharide challenges was lower in progesterone-treated than in 17beta-estradiol-treated steers. Xanthine oxidase response to either challenge was greater for estradiol-treated steers. PMID: 21356584
    19. The expression and cellular localization of tumor necrosis factor-alpha (TNF) and its receptors (TNFRI and TNFRII) mRNAs and proteins, were determined. PMID: 20705117
    20. Gene expression changes were observed, but there were no changes in TNFalpha concentrations, which may indicate its local involvement in catabolic adaptation of adipose tissue. PMID: 20579185
    21. ALOX5AP, CPNE3, IL1R2, IL6, TLR2, TLR4, and THY1 were upregulated in blood polymorphonuclear cells in negative energy balance versus positive energy balance cows. PMID: 20072847
    22. The upregulation of TNFRI mRNA expression by IFNG suggests that TNF and IFNG synergistically affect the death of luteal endothelial cells resulting in acute luteolysis PMID: 20562522
    23. aggrecanase activity (a) is responsible for early TNFalpha-dependent aggrecan cleavage and GAG release in the meniscus and (b) might be involved in meniscal degeneration. PMID: 19778432
    24. Tumour necrosis factor alpha up-regulates protein kinase R (PKR)-activating protein (PACT) and increases phosphorylation of PKR and eukaryotic initiation factor 2-alpha in articular chondrocytes PMID: 12440939
    25. results support the possibility that tumor necrosis factor-alpha is responsible for the changes in hormone secretion, milk production and composition, and inflammatory parameters observed during coliform mastitis PMID: 12703618
    26. TNF-alpha exerts transcriptional, as well as post-transcriptional, effects on eNOS gene expression PMID: 14581470
    27. there are TNFalpha receptors in bovine granulosa and theca cells in small, preovulatory and atretic follicles; TNFalpha plays a role in regulating their secretory function PMID: 14967894
    28. TNFalpha augments prostaglandin F2alpha and E2 production by bovine endometrial stromal cells partially via induction of NOS with subsequent stimulation of NO-cGMP formation PMID: 15226598
    29. TNF binding induces release of AIP1 (DAB2IP) from TNFR1, resulting in cytoplasmic translocation and concomitant formation of an intracellular signaling complex comprised of TRADD, RIP1, TRAF2, and AIPl. PMID: 15310755
    30. Levels in milk are altered in the presence of bacteria PMID: 15372997
    31. These results suggest that the pattern of plasma TNF-alpha changes that evolve from a low-level double LPS challenge. PMID: 15661839
    32. TNFalpha-induced desumoylation and cytoplasmic translocation of HIPK1 are critical in TNFalpha-induced ASK1-JNK/p38 activation PMID: 15701637
    33. PKD is a critical mediator in H2O2- but not TNF-induced ASK1-JNK signaling PMID: 15755722
    34. Dok-4 has a role as an anchoring molecule for the tyrosine kinase c-Src, and in turn as a regulator of TNF-alpha-mediated ROS production and NF-kappaB activation PMID: 15855164
    35. data suggest that an aberrant expression of TNF-alpha might contribute to the progression of bovine leukosis in animals which develop persistent lymphocytosis of B-cells or B-cell lymphosarcoma PMID: 16155729
    36. the p38-MAPK/CREB pathway plays a critical role in TNFalpha-induced VCAM-1 expression PMID: 16715652
    37. frequency of the TNF-824G allele was higher in cows with bovine leukemia virus -induced lymphoma than asymptomatic carriers; data suggest the polymorphism in the promoter region could in part contribute to progression of lymphoma in BLV-infection PMID: 16839795
    38. In endothelial cells TNF-alpha coordinately downregulates nitric oxide synthase (eNOS) and argininosuccinate synthase expression, resulting in a severely impaired citrulline-NO cycle. PMID: 17496212
    39. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected. PMID: 17530193
    40. Lipopolysaccharide stimulation up-regulates the secretion of cytokines by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
    41. the pathogen causing subclinical mastitis impairs NF-kappaB activation in MEC thereby severely weakening the immune response,induction of IL-8 and TNFalpha, in the udder PMID: 17936907
    42. Tumor necrosis factor alpha inhibits oxidative phosphorylation through tyrosine phosphorylation at subunit I of cytochrome c oxidase PMID: 18534980
    43. These results indicate that activation of the intrinsic antistaphylococcal response in bovine endothelial cells (BEC), enhanced by TNF-alpha and IL-1beta, is effective to eliminate S. aureus and S. epidermidis. PMID: 18625014
    44. These results suggest that activation of the signaling pathway initiated by TNF-alpha could play an important role in the phagocytosis of Staphylococcus epidermidis. PMID: 19191876
    45. TNFalpha seems to play some role as a modulator of PGF(2alpha) and PGE(2) production and for transferring the embryo from the oviduct to the uterus by stimulating NO production in the bovine oviduct. PMID: 19596830
    46. The current study suggests the existence of an active TNF-alpha-plasminogen-plasmin autocrine/paracrine loop on the massively infiltrated polymorphonuclear neutrophils inside udders of drying-off cows. PMID: 19638263
    47. Mechanical injury potentiates the catabolic effects of TNFalpha and IL-6/sIL-6R in causing proteoglycan degradation in human and bovine cartilage. PMID: 19790045

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 数据库链接：