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Bovine Vitamin A (VA) ELISA Kit

  • 中文名称:
    牛维生素A(VA)酶联免疫试剂盒
  • 货号:
    CSB-E17987B
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
        VA(Vitronectin)是一种在体内广泛存在的糖蛋白,与许多生物学过程有关,如凝血、细胞外基质的重构以及炎症反应等。VA可以结合到细胞表面上的特定受体中,参与了许多信号通路的调控。在一些疾病中,VA的含量和活性也发生了改变,如某些癌症和心血管疾病
        j9九游会登录入口首页生物所提供的Bovine Vitamin A (VA) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测牛血清、血浆、组织匀浆样本中的VA,其灵敏度为4.672 ng/mL,检测范围为6.25 ng/mL-100 ng/mL。
     
  • 缩写:
    VA
  • 种属:
    Bos taurus (Bovine)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    6.25 ng/mL-100 ng/mL
  • 灵敏度:
    4.672 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 95  
    Range % 91-99  
    1:20 Average % 103  
    Range % 99-107  
    1:40 Average % 98  
    Range % 93-103  
    1:80 Average % 92  
    Range % 89-95  
  • 回收率:
    The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 93-104  
    EDTA plasma (n=4) 89 83-95  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.098 0.095 0.097    
    50 0.150 0.163 0.157    
    25 0.263 0.268 0.266    
    12.5 0.551 0.565 0.558    
    6.25 1.037 1.065 1.051    
    0 2.266 2.287 2.277    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    最新研究表明,VA蛋白在细胞分化、免疫应答和炎症反应等生物学过程中起着重要的作用。