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Bovine Vitamin D-binding protein(GC) ELISA kit

  • 中文名称：
    牛维生素D结合蛋白(GC)酶联免疫试剂盒
  • 货号：
    CSB-EL009306BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine Vitamin D-binding protein (GC) ELISA Kit was designed for the quantitative measurement of Bovine Vitamin D-binding protein (GC) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

  • 别名：
    GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
  • 缩写：
    GC
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 μg/mL-10 μg/mL
  • 灵敏度：
    0.039 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 86  
    Range % 82-92  
    1:200 Average % 93  
    Range % 89-97  
    1:400 Average % 92  
    Range % 84-96  
    1:800 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 103 100-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.103 0.104 0.104    
    5 0.151 0.155 0.153    
    2.5 0.275 0.292 0.284    
    1.25 0.537 0.540 0.539    
    0.625 0.981 0.923 0.952    
    0.312 1.246 1.328 1.287    
    0.156 1.588 1.724 1.656    
    0 2.424 2.613 2.519    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
  • 基因功能参考文献：
    1. Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production. PMID: 27760518
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 数据库链接：