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  4. Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Bovine lipopolysaccharide binding protein (LBP) ELISA kit

  • 中文名称：
    牛脂多糖结合蛋白(LBP)酶联免疫试剂盒
  • 货号：
    CSB-E16370B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine LBP ELISA Kit was designed for the quantitative measurement of Bovine LBP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-102  
    1:2 Average % 86  
    Range % 82-95  
    1:4 Average % 98  
    Range % 90-107  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-96  
    EDTA plasma (n=4) 95 90-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.126 2.150 2.138 2.002  
    500 1.611 1.623 1.617 1.481  
    250 1.142 1.158 1.150 1.014  
    125 0.797 0.780 0.789 0.653  
    62.5 0.463 0.451 0.457 0.321  
    31.25 0.340 0.334 0.337 0.201  
    15.6 0.271 0.275 0.273 0.137  
    0 0.135 0.136 0.136 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am interested in the product CSB-E16370B and would like to confirm:
Has this kit actually been validated with bovine samples, or is it based off a sequence homology?

A:
Thanks for your inquiry.
We tested bovine serum and plasma samples before and the test value is 15.6-62.5ng/ml.
We suggest you use the kit for the corresponding species to test your samples.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
  • 基因功能参考文献：
    1. Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID: 26813383
    2. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
    3. One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID: 25700343
    4. Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID: 22733492
  • 亚细胞定位：
    Secreted. Cytoplasmic granule membrane.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 数据库链接：